Cochlear electrophysiology and histopathology of rats exposed to intratympanic silica nanoparticles
Cochlear electrophysiology and histopathology of rats exposed to intratympanic silica nanoparticles
Toxic effects of silica nanoparticle of rats exposed intratympanically for 7 days were investigated using brainstem auditory evoked potential and distortion product otoacoustic emission techniques. The histopathological changes were evaluated with light microscopy and field emission-scanning electron microscopy were demonstrated in cochleas using based energy-dispersive X-ray spectroscopy. The reduction in the amplitude and prolongation at the peak latency and interpeak latencies of brainstem auditory evoked potential were observed. Silica nanoparticles significantly reduced distortion product otoacoustic emission amplitude in the 2, 3 and 4 kHz frequencies. In the light microscopic evaluation, the samples obtained from the experimental group showed a significant loss of hair cells. Field emission scanning electron microscope analysis of experimental group showed considerable hair cell damage compared to control group. The findings of this study show that intratympanic administration of silica nanoparticles may lead to hearing impairment by causing structural changes in cochlear hair cells.
___
- Murugadoss S, Lison D, Godderis L, Van Den Brule S, Mast J, Brassinne F, Sebaihi N, Hoet PH. Toxicology of silica nanoparticles: an update. ArchToxicol. 2017; 91(9): 2967-3010. [CrossRef]
- Maser E, Schulz M, Sauer UG, Wiemann M, Ma-Hock L, Wohlleben W, Hartwig A, Landsiedel R. In vitro and in vivo genotoxicity investigations of differently sized amorphous SiO2 nanomaterials. Mutat Res Genet Toxicol Environ Mutagen. 2015; 794: 57-74. [CrossRef]
- Du Z, Zhao D, Jing L, Cui G, Jin M, Li Y, Xiaomei L Ying L, Haiying D, Caixia G, Xianging Z, Zhiewi S. Cardiovascular toxicity of different sizes amorphous silica nanoparticles in rats after intratracheal instillation. Cardiovasc Toxicol. 2013; 13(3): 194-207. [CrossRef]
- Shrivastava, R, Raza S, Yadav A, Kushwaha P, Flora SJ. Effects of sub-acute exposure to TiO2, ZnO and Al2O3 nanoparticles on oxidative stress and histological changes in mouse liver and brain. Drug and Chem Toxicol. 2014; 37(3): 336-347. [CrossRef]
- Duan, J, Yu Y, Li Y, li Y, Liu H, Jing L, Yang M, Jing L, Yang M, Wang J, Chunqi L, Zhiewi S. Low dose exposure of silica nanoparticles induces cardiac dysfunction via neutrophil mediated inflammation and cardiac contraction in zebrafish embryos. Nanotoxicology. 2016; 10(5): 575-585. [CrossRef]
- Breznan D, Das DD, O'Brien JS, MacKinnon-Roy C, Nimesh S, Vuong NQ, Bernatchez S, De Silva N, Hill M, Kumarathasan P, Vincent R. Differential cytotoxic and inflammatory potency of amorphous silicon dioxide nanoparticles of similar size in multiple cell lines. Nanotoxicology. 2017; 11(2): 223-235. [CrossRef]
- Yang, M, Jing L, Wang J, Yu Y, Cao L, Zhang L, Zhou X, Sun Z. Macrophages participate in local and systemic inflammation induced by amorphous silica nanoparticles through intratracheal instillation. Int J Nanomedicine. 2016; 11: 6217-6228. [CrossRef]
- Tarantini A, Lanceleur R, Mourot A, Lavault MT, Casterou G, Hogeveen K, Fessard V. Toxicity, genotoxicity and proinflammatory effects of amorphous nanosilica in the human intestinal Caco-2 cell line. Toxicol in Vitro. 2015b; 29(2): 398– 407. [CrossRef]
- Guo C, Yang M, Jing L, Wang J, Yu Y, Li Y, Duan J, Zhou X, Li Y, Sun Z. Amorphous silica nanoparticles trigger vascular endothelial cell injury through apoptosis and autophagy via reactive oxygen species-mediated MAPK/Bcl2 and PI3K/Akt/mTOR signalling. Int J Nanomedicine. 2016; 11: 5257-5276. [CrossRef]
- Yu Y, Li Y, Wang W, Jin M, Du Z, Li Y, Duan J. Yu Y, Sun Z. Acute toxicity of amorphous silica nanoparticles in intravenously exposed ICR mice. PloS one. 2013; 8(4): e61346. [CrossRef]
- Nemmar A, Yuvaraju P, Beegam S, Yasin J, Kazzam EE, Ali BH. Oxidative stress, inflammation, and DNA damage in multiple organs of mice acutely exposed to amorphous silica nanoparticles. Int J Nanomedicine. 2016; 11: 919-28. [CrossRef]
- Di Cristo L, Movia D, Bianchi MG, Allegri M, Mohamed BM, Bell AP, Moore C. Pinelli S, Rasmussen K, Sintes JR, Mello AP, Bussaloti O, Bergamaschi E. Proinflammatory effects of pyrogenic and precipitated amorphous silica nanoparticles in innate immunity cells. Toxicol Sci. 2015; 150(1): 40-53. [CrossRef]
- Li L, Chao T, Brant J, O'Malley B, Tsourkas Jr A, Li D. Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss. Adv Drug Deliv Rev. 2017; 108: 2-12. [CrossRef]
- Pyykkö I, Zou J, Schrott-Fischer A, Glueckert R, Kinnunen P. An overview of nanoparticle based delivery for treatment of inner ear disorders. In Sokolowski B (Ed). Auditory and Vestibular Research. Humana Press, New York, 2016, pp. 363-415.
- Praetorius M, Brunner C, Lehnert B, Klingmann C, Schmidt H, Staecker H, Schick B. Transsynaptic delivery of nanoparticles to the central auditory nervous system. Acta Otolaryngol, 2007; 127(5): 486-490. [CrossRef]
- Ivanov S, Zhuravsky S, Yukina G, Tomson V, Korolev D, Galagudza M. In vivo toxicity of intravenously administered silica and silicon nanoparticles. Materials 2012; 5(10): 1873-1889. [CrossRef]
- Kelly JB, Masterton B. Auditory sensitivity of the albino rat. J Comp Physiol Psychol. 1977; 91(4): 930-936.
- Albuquerque AAS, Rossato M, de Oliveira JAA, Hyppolito MA. Understanding the anatomy of ears from guinea pigs and rats and its use in basic otologic research. Brazi J Otorhinolaryngo. 2009; 75(1): 43-49.
- Celesia GG. Brainstem auditory evoked responses. In: Celesia GG. (Ed). Disorders of peripheral and central auditory processing. Elsevier Health Sciences Netherlands, 2013. pp. 137-154.
- Rozhkov VP, Soroko SI. Age-and sex-related differences in brainstem auditory evoked potentials in secondary school students living in Northern European Russia. Hum Physiol. 2009; 35(6): 703–713.
- Abdala C, Visser-Dumont L. Distortion product otoacoustic emissions: A tool for hearing assessment and scientific study. Volta Rev. 2001; 103(4): 281-302.
- Nishimori H, Kondoh M, Isoda K, Tsunoda S, Tsutsumi Y, Yagi K. Silica nanoparticles as hepatotoxicants. Eur J Pharm Biopharm. 2009; 72(3): 496-501. [CrossRef]
- Simonin M, Martins JM, Uzu G, Vince E, Richaume A. Combined study of titanium dioxide nanoparticle transport and toxicity on microbial nitrifying communities under single and repeated exposures in soil columns. Environ Sci Technol. 2016; 50(19): 10693-10699. [CrossRef]
- Montgomery SC, Cox BC. Whole mount dissection and immunofluorescence of the adult mouse cochlea. J Vis Exp. 2016; 107: e(53561).