Stabilization of hydrochlorothiazide nanocrystals using fibroin
Stabilization of hydrochlorothiazide nanocrystals using fibroin
Nanocrystals of the poorly water soluble drugs is a promising strategy. To stabilize the drug nanocrystals, stabilizers are usually used; however, the use of common stabilizers is limited by weak stabilization effect and toxicological concerns for long-term treatment. The present work was aimed to investigate the potential of natural silk protein as novel stabilizer for nanocrystal of hydrochlorothiazide, which was a model drug. The nanocrystals of drug with hydrophobic protein stabilizer were prepared by antisolvent precipitation method. Prepared nanocrystals were evaluated for parameters like particle size, zeta potential, DSC, XRD, %crystallinity, SEM, drug content and in vitro dissolution test. Stabilization efficiency of nanocrystals was assessed by their % crystallinity for 3 months. Optimized batch R2 was shown smaller particle size, highest drug content and drug dissolution. Nanocrystals were shown the extended release due to coat of fibroin around the hydrochlorothiazide. From the % crystallinity study it was found that there was not significant change in the nanocrystals prepared using fibroin. So, it was concluded that the fibroin is good stabilizer for drug nanocrystals.
___
- Katteboina S, Chandrasekhar P, Balaji S. Drug nanocrystals: a novel formulation approach for poorly soluble drugs. Int J Pharm Tech Res. 2009; 1(3): 682-694.
- Patravale V, Date A, Kulkarni R. Nanosuspensions a promising drug delivery strategy. J Pharm Pharmacol. 2004; 56: 827-840. [CrossRef]
- Kale M,Mishra B. Drug nanocrystals: A way toward scale-up. Saudi Pharm J. 2016; 24(4): 386-404.[CrossRef]
- Pawar V,Singh Y, Meher J, Gupta S, Chourasiya M. Engineered nanocrystal technology: in-vivo fate, targeting and applications in drug delivery. J Control Release. 2014; 183: 51-66. [CrossRef]
- Buraphacheep V, Morakul B. Nanocrystals for enhancement of oral bioavailability of poorly water-soluble drugs. Asian J Pharm Sci. 2015; 13-23. [CrossRef]
- Wei H, Ye L, Jianping Q, Lingyun C, Fuqiang H, Wei W. Food proteins as novel nanosuspension stabilizers for poorly water-soluble drugs. Int J Pharm. 2013; 441: 269-278. [CrossRef]
- Cheng Y, Koh LD, Li D, Ji B, Hang MY, Zhang YW. On the strength of β-sheet crystallites of Bombyx mori silk fibroin. J R Soc Interface. 2014; 11: 1-9. [CrossRef]
- Bajaj A, Rao M, Pardeshi A, Sali D. Nanocrystallization by Evaporative Antisolvent Technique for Solubility and Bioavailability Enhancement of Telmisartan. AAPS Pharm Sci Tech. 2012; 13(4): 1331-1340. [CrossRef]
- Nanjwade B, Derkar G, Bechra H,Nanjwade V, Mani F. Design and Characterization of Nanocrystals of Lovastatin for Solubility and Dissolution Enhancement. J Nanomed Nanotechnol. 2011; 2: 107. [CrossRef]
- Choi M, Briançon S, Andrieu J, Min S, Fessi H. Effect of freeze drying process conditions on the stability of nanoparticles. Dry Technol. 2004; 22: 335-346. [CrossRef]
- Kakran M, Shegokar R, Sahoo NG, Shaal LA, Li L, Muller RH. Fabrication of quercetin nanocrystals: comparison of different methods. Eur J Pharm Biopharm. 2010; 80: 113-121. [CrossRef]
- Lee J, Lee SJ, Choi JY, Yoo JY, Ahn CH. Amphiphilic amino acid copolymers as stabilizers for the preparation of nanocrystal dispersion. EurJ Pharm Sci 2005; 24(5): 441–449. [CrossRef]
- Patel AP, Patel JK, Patel KS, Deshmukh AB, Mishra BR. A review on drug nanocrystal a carrier free drug delivery. Int J Res Ayurveda Pharm. 2(2), 448-458 (2011).
- Peltonen, L. Hirvonen J. Pharmaceutical nanocrystals by nanomilling: critical process parameters, particle fracturing and stabilization methods. J Pharm Pharmacol. 2010; 62(11): 1569-1579. [CrossRef]