Prapairat SEEPHONKAI, Natwipha MONGKOLSIRI, Wiranya THIABPHET, Rattanaphorn TRAISATHIT, Sutthira SEDLAK, Komgrit WONGPAKAM, Taweesak DHAMMARAJ, Aphidech SANGDEE

Antioxidant, xanthine oxidase inhibitory and antibacterialactivities of selected galactogogue Thai medicinal plantwater and ethyl acetate extracts

Water and ethyl acetate extracts of sixteen galactogogue Thai medicinal plants collected in Northeastern Thailand were evaluated for the antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging property and total phenolic content (TPC), xanthine oxidase inhibitory and antibacterial activities. Among the extracts, both of the water and ethyl acetate extracts from the stem bark of Caesalpinia sappan (CS) and Ochna integerrima (OI) exhibited potent DPPH radical scavenging capacity (IC50 9.47 ± 0.59 13.25 ± 0.52 g/mL) as compared to ascorbic acid (IC50 5.39 ± 0.32 g/mL) and high TPC (632.89 ± 10.18 985.34 ± 0.76 mg gallic acid equivalent (GAE)/g of extract). The strongest xanthine oxidase inhibitory property was detected in the ethyl acetate extract from the root of Clausena harmandiana (CH) followed by CS and OI extracts showing %inhibition of 99.22 ± 0.22, 78.00 ± 0.79 and 68.67 ± 1.14, respectively, at the tested concentration of 50 µg/mL and referenced to allopurinol. The water extracts of CS and OI also possessed good antibacterial activity against eight tested pathogenic bacteria especially Gram-positive, S. aureus(MSSA) DMST 2933, S. aureus (MRSA) DMST 20651 and Bacillus cereus ATCC 11778 , with the MIC and MBC values ranging from 0.39 1.56 mg/mL. The antibacterial property of OI extract and xanthine oxidase inhibitory activity of CH and OI extracts were first reported.

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[1] Newman DJ, Cragg GM. Natural products as source of new drugs from 1981-2014. J Nat Prod. 2016; 79: 629-661. [CrossRef]
[2] Rajagopal PL, Premaletha K, Kiron SS, Sreejith KR. Phytochemical and pharmacological review on Cassia fistulaLinn. The golden shower. Int J Pharm Biol Sci. 2013; 3: 672-679.
[3] Mortel M, Mehta SD. Systematic review of the efficacy of herbal galactogogues. J Hum Lact. 2013; 29(2): 154-162. [CrossRef]
[4] Gutiérrez-Grijalva EP, Picos-Salas MA, Leyva-López N, Criollo-Mendoza MS, Vazquez-Olivo G, Heredia JB. Flavonoids and phenolic acids from oregano: Occurrence, biological activity and health benefits. Plants. 2018; 7(2): 1- 23. [CrossRef]
[5] Jungbauer A, Medjakovic S. Anti-inflammatory properties of culinary herbs and spices that ameliorate the effects of metabolic syndrome. Maturitas. 2012; 71: 227-239. [CrossRef]
[6] Goncalves S, Moreira E, Grosso C, Andrade PB, Valentao P, Romano A. Phenolic profile, antioxidant activity and enzyme inhibitory activities of extracts from aromatic plants used in mediterranean diet. J Food Sci Technol. 2017; 54: 219-227.
[7] Lin LZ, Yang QY, Zhao K, Zhao MM. Identification of the three phenolic profile of adlay bran by UPLC-QTOFMS/MS and inhibitory mechanisms of phenolic acids against xanthine oxidase. Food Chem. 2018; 253: 108-118. [CrossRef]
[8] Lin L, Liu X, Zhao M. Screening of xanthine oxidase inhibitor from selected deible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats. J Funct Foods. 2018; 50: 26-36. [CrossRef]
[9] Perez-Ruiz F, Dalbeth N, Bardin T. A review of uric acid, crystal deposition disease, and gout. Adv Ther. 2015; 32(1): 31-41.
[10] Zhang HJ, Li LN, Zhou J, Yang QQ, Liu PG, Xu P, Liang WQ, Cheng L, Zhang YQ, Pu JB, Hu, YJ. Effects of Gnaphalium affine D. Don on hyperuricemia and acute gouty arthritis. J Ethnopharm. 2017; 203: 304-311. [CrossRef]
[11] Liu LM, Cheng SF, Shieh PC, Lee JC, Chen JJ, Ho CT, Kuo SC, Huo DH, Huang LJ, Way TD. The methanol extract of Euonymus laxiflorus, Rubia lanceolate and Gardenia jasminoides inhibits xanthine oxidase and reduce serum uric acid level in rat. Food Chem Toxicol. 2014; 70: 179-184. [CrossRef]
[12] Nile SH, Nile AS, Keum YS, Sharma K. Utilization of quercetin and quercetin glycosides from onion (Allium cepa L.) solid waste as an antioxidant, urease and xanthine oxidase inhibitors. Food Chem, 2017; 235: 119-126. [CrossRef]
[13] Pacher P, Nivorozhkin A, Szabo C. Therapeutic effect of xanthine oxidase inhibitors: Renaissance half a century after the discovery of allopurinol. Pharmacol Rev. 2006; 58(1): 87-114.
[14] Bouarab-Chibane L, Degraeve P, Ferhout H, Bouajila J, Oulahal N. Plant antimicrobial polyphenols as potential natural food preservatives. J Sci Food Agric. 2019; 99(4): 1457-1474. [CrossRef]
[15] Bouarab-Chibane L, Forquet V, Lantéri P, Clément Y, Léonard-Akkari L, Oulahal, N, Degraeve P, Bordes C. Antibacterial properties of polyphenols: Characterization and QSAR (Quantitative Structure-Activity Relationship) models. Front Microbiol. 2019; 10. [CrossRef]
[16] Cushnie TPT, Hamilton VES, Chapman DG, Taylor PW, Lamb AJ. Aggregation of Staphylococcus aureus following treatment with the antibacterial flavonol galangin. J Appl Microbiol. 2007; 103(5): 1562-1567. [CrossRef]
[17] Cŏté J, Caillet S, Doyon G, Sylvain JF, Lacroix M. Bioactive compounds in cranberries and their biological properties. Crit Rev Food Sci Nutr. 2010; 50(7): 666-679. [CrossRef]
[18] Nirmal NP, Rajput MS, Prasad RGSV, Ahmad M. Brazillin from Caesalpinia sappan heartwood and its pharmacological activities: A review. APJTM. 2015; 8(6): 421-430. [CrossRef]
[19] Toegel S, Wu SQ, Otero M, Goldring MB, Leelapornpisid P, Chiari C, Kolb A, Unger FM, Windhager R, Viernstein H. Caesalpinia sappan extract inhibits IL1b-mediated overexpression of matrix metalloproteinases in human chondrocytes. Genes Nutr. 2012; 7(2): 302-318. [CrossRef]
[20] Wetwitayaklung P, Phaechamud T, Keokitichai S. Antioxidant activity of Caesalpinia sappan L. heartwood in various ages. NUJST. 2005; 13(2): 43-52.
[21] Pensec F, Szakiel A, Pączkowski C, Woźniak A, Grabarczyk M, Bertsch C, Fischer MJC, Chong J. Characterization of triterpenoid profiles and triterpene synthase expression in the leaves of eight Vitis vinifera cultivars grown in the Upper Rhine Valley. Int J Plant Res. 2016; 129(3): 499-512.
[22] Batubara I, Mitsunaga T, Ohashi H. Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities. J Wood Sci. 2009; 55(3): 230-235. [CrossRef]
[23] Sasaki Y, Hosokawa T, Nagai M,Nagumo S. In vitro study for inhibition of NO production about constituents of Sappan lignum. Biol Pharm Bull. 2007; 30(1): 193-196. [CrossRef]
[24] Promden W, Viriyabancha W, Monthakantirat O, De-Eknamkul W. Investigation of in-vitro antioxidant potential of Thai medicinal plant extracts. 6th Thailand-Japan International Academic Conference 2013. Osaka University, Osaka, Japan; 2013.
[25] Ghadage DM, Kshirsagar PR, Pai SR, Chavan JJ. Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) – An important underutilized plant. Biochem Biophys Rep. 2017; 12: 79-90. [CrossRef]
[26] Reutrakul V, Ningnuek N, Pohmakotr M, Yoosook C, Napaswad C, Kasisit J, Santisuk T, Tuchinda P. Anti HIV-1 flavonoid glycosides from Ochna integerrima. Planta Med. 2007; 73(7): 683-688.
[27] Ichino C, Kiyohara H, Soonthornchareonnon N, Chuakul W, Ishiyama A, Sekiguchi H, Namatame M, Otoguro K, Omura S, Yamada H. Antimalarial activity of biflavonoids from Ochna integerrima. Planta Med. 2006; 72(7): 611-614.
[28] Kaewamatawong R, Likhitwitayawuid K, Ruangrungsi N, Takayama H, Kitajima M, Aimi N. Novel biflavonoids from the stem bark of Ochna integerrima. J Nat Prod. 2002; 65(7): 1027-1029. [CrossRef]
[29] Thongthoom T, Songsiang U, Phaosiri C, Yenjai C. Biological activity of chemical constituents from Clausena harmandiana. Arch Pharmacal Res. 2010; 33(5): 675-680.
[30] Nguyen MTT, Awale S, Tezuka Y, Tran QL, Kadota S. Xanthine Oxidase Inhibitors from the Heartwood of Vietnamese Caesalpinia sappan. Chem Pharm Bull. 2005; 53(8): 984-988. [CrossRef]
[31] Yenjai C, Sripontan S, Sriprajun P, Kittakoop P, Jintasirikul A, Tanticharoen M, Thebtaranonth Y. Coumarins and carbazoles with antiplasmodial activity from Clausena harmandiana. Planta Med. 2000; 66(3): 277-279.
[32] Fais A, Era B, Asthana S, Sogos V, Medda R, Santana L, Uriarte E, Matos MJ, Delogu F, Kumar A. Coumarin derivatives as promising xanthine oxidase inhibitors. Int J Biol Macromol. 2018; 120(Pt A): 1286-1293. [CrossRef]
[33] Ojha R. An updated patent review: xanthine oxidase inhibitors for the treatment of hyperuricemia and gout (2011- 2015). Expert Opin Ther Pat. 2017; 27(3): 311-345. [CrossRef]
[34] Knolker HJ and Reddy KR. Isolation and synthesis of biologically active carbazole alkaloids. Chem Rev. 2002; 102(11): 4303-4428. [CrossRef]
[35] Johnston MD, Hanlon GW, Denyer SP, Lambert RJW. Membrane damage to bacteria caused by single and combined biocides. J Appl Microbiol. 2003; 94(6): 1015-1023. [CrossRef]
[36] Delaquis PJ, Stanich K, Girard B, Mazza G. Antimicrobial activity of individual and mixed fractions of drill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol. 2002; 74(1-2): 101-109. [CrossRef]
[37] Cushnie TPT, O’Driscoll NH, Lamb AJ. Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action. Cell Mol Life Sci. 2016; 73(23): 4471-4492.
[38] Nirmal NP, Pinichayupakaranant P. Anti-Propionibacterium acnes assay-guided purification of brazilin and preparation of brazilin rich extract from Caesalpinia sappan heartwood. Pharm Biol. 2014; 52(9): 1204-1207. [CrossRef]
[39] Srinivasan R, Selvam GG, Karthik S, Mathivanan K, Baskaran R, Karthikeya M, Gopi M, Govindasamy C. In vitro antimicrobial activity of Caesalpinia sappan L. Asian Pac J Trop Biomed. 2012; 2(1): S136-S139. [CrossRef]
[40] Kim KJ, Yu HH, Jeong SI, Cha JD, Kim SM, You YO. Inhibitory effects of Caesalpinia sappan on growth and invasion of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol. 2004; 91(1): 81-87. [CrossRef]
[41] Xu HX and Lee SF. The antibacterial principle of Caesalpinia sappan. Phytother Res. 2004; 18(8): 647-651. [CrossRef]
[42] Seephonkai P, Samchai S, Thongsom A, Sunaart S, Kiemsanmuang B, Chakuton K. DPPH radical scavenging activity and total phenolics of Phellinus mushroom extracts collected from northeast Thailand. Chin J Nat Med. 2011; 9(6): 441- 445. [CrossRef]
[43] Gawlik-Dziki U, Dziki D, Świeca M, Nowak R. Mechanism of action and interactions between xanthine oxidase inhibitors derived from natural sources of chlorogenic and ferulic acids. Food Chem. 2017; 225: 138-145. [CrossRef]
[44] Shintani H. Determination of xanthine oxidase. Pharm Anal Acta. 2013; S7.
[45] Sangdee K, Nakbanpote W, Sandee A. Isolation of the entomopathogenic fungal strain Cod-MK1201 from a cicada nymph and assessment of its antibacterial activities. Int J Med Mushrooms. 2015; 17(1): 51-63.
[46] Sangdee A, Sangdee K, Buranrat B, Thammawat S. Effects of mycelial extract and crude protein of the medicinal mushroom, Ophiocordyceps sobolifera, on the pathogenic fungus, Candida albicans. Trop J Pharm Res. 2018; 17(12): 2449-