Antioxidant, anti-inflammatory and wound healing properties of ethanolic extracts of Thevetia peruviana (Pers.) K. Schum

Thevetia peruviana (Pers.) K. Schum, well known as Karvira in the Ayurvedic text. The plant has been traditionally used for the treatment of wounds, inflammation and diverse types of skin disorders. The present study was designed to analyze the antioxidant and anti-inflammatory mediated wound healing potential of T. peruviana leaf (TPLE) and fruit rind (TPFE) ethanolic extracts. To establish the efficacy, the study was designed using in vitro antioxidant activities, carrageenan-induced inflammation model and incision, excision, dead space wound models associated with antioxidant enzymes, tissue biochemical and inflammatory marker analysis allied with acute toxicity studies in rats. The serum and tissue biochemical, histological and organ index studies of acute toxicity evaluation confirms the non-toxic nature of the extracts. The TPLE and TPFE treated groups represents complete restoration of normal skin structures after biopsy at day 14 comparable to the control group healed at day 24, the significant amount of hydroxyproline, hexosamine, hexuronic acid observed in dead space wound study, maximum wound breaking strength is intern related with the more collagen and fibroblast deposition can be correlated with the excellent wound healing efficiency of extracts. Consequently, the profound in vitro antioxidant efficacy, lowing of carrageenan induced inflammation, ESR, nitric oxide radicles and LPO (lipid peroxidation) content allied with an elevation of the GSH (reduced glutathione), SOD (superoxide dismutase), CAT (catalase), and GPx (glutathione peroxidase) in granuloma is a clear indication of good antioxidant and anti-inflammatory activity of TPLE and TPFE extracts. Our study provides evidence for the association of the excellent antioxidant, anti-inflammatory and wound healing capacity of T. peruviana present in a prudently balanced means which is required in a novel wound healing drug.

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Leach MJ. Making sense of the venous leg ulcer debate: A literature review. J Wound Care. 2004; 13(2): 52-56. [CrossRef]
Gupta N, Jain UK. Prominent wound healing properties of indigenous medicines. J Nat Pharm. 2010; 1 (1).
Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, Leaper D, Georgopoulos NT. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS‐modulating technologies for augmentation of the healing process. Int Wound J 2015. [CrossRef]
dos Santos Gramma LS, Marques FM, Vittorazzi C, de Andrade TA, Frade MA, de Andrade TU, Endringer DC, Scherer R, Fronza M. Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing. J Ethnopharmacol. 2016; 190: 319-327. [CrossRef]
Dwivedi D, Dwivedi M, Malviya S, Singh V. Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complementary Med. 2016; 1-7. [CrossRef]
Chaudhari A, Singh, B. A Critical Review of Karvira (Nerium indicum Mill). Int J Ayurveda & Med Sci. 2016; 1(2): 5155.
Rahman N, Rahman H, Haris M, Mahmood R. Wound healing potentials of Thevetia peruviana: Antioxidants and inflammatory markers criteria. J Tradit Complementary Med. 2017; 7(4): 519-525. [CrossRef]
Rahman N, Mahmood R, Rahman H, Haris M. Systematic screening for phytochemicals of various solvent extracts of Thevetia peruviana Schum. leaves and fruit rind. Int J Pharm Pharm Sci. 2014; 6(8): 173-179.
Nesy EA, Mathew L. A comparative evaluation of antimicrobial and antioxidant properties of Thevetia neriifolia, Juss fruit rind extracts. Int J Curr Pharm Res. 2014; 6: 47-50.
De M, Sharma AK. Cardenolide contents in different genotypes of Thevetia neriifolia and Nerium odorum. Nucleus. India. 1980; 23(3): 213-225.
Abe F, Iwase Y, Yamauchi T, Yahara S, Nohara T. Flavonol sinapoyl glycosides from leaves of Thevetia peruviana. Phytochemistry. 1995; 40: 577-581. [CrossRef]
Tewtrakul S, Nakamura N, Hattori M, Fujiwara T, Supavita T. Flavanone and flavonol glycosides from the leaves of Thevetia peruviana and their HIV-1 reverse transcriptase and HIV-1 integrase inhibitory activities. Chem Pharm Bull. 2002; 50(5): 630-635. [CrossRef]
Gata-Gonçalves L, Nogueira JMF, Matos O, de Sousa RB. Photoactive extracts from Thevetia peruviana with antifungal properties against Cladosporium cucumerinum. J Photochem Photobiol B. 2003; 70(1): 51-54. [CrossRef]
Miyagawa T, Ohtsuki T, Koyano T, Kowithayakorn T, Ishibashi M. Cardenolide glycosides of Thevetia peruviana and triterpenoid saponins of Sapindus emarginatus as TRAIL resistance-overcoming compounds. J Nat Prod. 2009; 72(8): 1507-1511. [CrossRef]
Mathuravalli K, and Lakshmi RE. Analysis of phytochemical components and anti-microbial activity of the toxic plant-Thevetia Peruviana. Indian J Innovations Dev. 2012; 1(2): 97-101.
Kohls S, Scholz-Böttcher BM, Teske J, Zark P, Rullkötter J. Cardiac glycosides from Yellow Oleander (Thevetia peruviana) seeds. Phytochemistry 2012; 75: 114-127. [CrossRef]
Sathish V, Umavathi S, Thangam Y, Mathivanan R. Analysis of phytochemical components and larvicidal activity of Thevetia peruviana (Pers) Merr, against the chickungunya vector Aedes aegypti (L). Int J Curr Microbiol App Sci. 2015; 4(1): 33-39.
Save SA, Lokhande RS, Chowdhary AS. Thevetia peruviana: अश्वाघना: the Good Luck tree. Innovations Pharm Pharmacother. 2015; 3: 586-606. [
Fitzmaurice SD, Sivamani RK, Isseroff RR. Antioxidant therapies for wound healing: a clinical guide to currently commercially available products. Skin Pharmacol Physiol. 2011; 24(3): 113-126. [CrossRef]
Nesy EA, Mathew L. Studies on antimicrobial and antioxidant efficacy of Thevetia neriifolia, Juss leaf extracts against human skin pathogens. Int J Pharm Sci Drug Res. 2014; 6: 164-168.
Rahman N, Mahmood R, Rahman H, Haris M. Spectrophotometric screening of potent bactericidal property of Thevetia peruviana Schum. leaf and fruit rind extracts on clinical and plant pathogens. Int J Appl Sci Biotechnol. 2014; 2: 451-459. [CrossRef]
Oluwasegun A, Nasiru A, John E. In vitro antioxidant potential of methanol extracts of Allamanda schottii Pohl and Thevetia peruviana (pers.) K. (Apocyanaceae). Asian J Plant Sci Res. 2015; 5(1): 10-13.
Tessou KZ, Lawson-Evi P, Metowogo K, Diallo A, Eklu-Gadegkeku K, Aklikokou K, Gbeassor M. Acute and subacute toxicity studies of Plumeria alba Linn.(Apocynaceae) hydroalcoholic extract in rat. Int J Biomed Sci. 2013; 9(4): 255.
Kurahashi T, Fujii J. Roles of antioxidative enzymes in wound healing. J Dev Biol. 2015; 3(2): 57-70. [CrossRef]
Posadas I, Bucci M, Roviezzo F, Rossi A, Parente L, Sautebin L, Cirino G.Carrageenan‐induced mouse paw oedema is biphasic, age‐weight dependent and displays differential nitric oxide cyclooxygenase‐2 expression. Br J Pharmacol. 2004; 142(2): 331-338. [CrossRef]
Pereira LD, Mota MR, Brizeno LA, Nogueira FC, Ferreira EG, Pereira MG, Assreuy AM. Modulator effect of a polysaccharide-rich extract from Caesalpinia ferrea stem barks in rat cutaneous wound healing : Role of TNF- α, IL-1 β. J Ethnopharmacol. 2016; 187: 213-223. [CrossRef]
Gamble JS, Fischer CEC, Flora of Presidency of Madras. 1915-1935, 3rd repr. Ed. Botanical Survey of India. Howrah, India, 1957.
Gouthamchandra K, Mahmood R, Manjunatha H. Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ. Toxicol Pharmacol. 2010; 30(1): 11-18. [CrossRef]
Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Food Chem. 2009; 113(4): 1202-1205. [CrossRef]
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: speciﬁc application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337–341. [CrossRef]
Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi. Pharm. J. 2013; 21(2): 143-152. [CrossRef]
Ruberto G, Baratta MT, Deans SG, Dorman HD. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med. 2000; 66(08): 687-693. [CrossRef]
Joseph JM, Sowndhararajan K, Manian S. Evaluation of analgesic and anti-inﬂammatory potential of Hedyotis puberula (G. Don) R. Br. ex Arn. in experimental animal models. Food Chem Toxicol. 2010; 48(7): 1876–1880. [CrossRef]
Jou JM, Lewis SM, Briggs C, LEE SH, De La Salle B, McFadden S. ICSH review of the measurement of the erythrocyte sedimentation rate. Int J Lab Hem. 2011; 33(2): 125-132. [CrossRef]