Plasmid-mediated colistin resistance mcr-1 in a clinical isolate of Escherichia coli ST19 in Algeria
We describe the emergence of plasmid-mediated colistin resistance mcr-1 in a clinical isolate of Escherichia coli ST19. The strain was isolated from a urine sample of a 75-year-old male patient. The identification was carried out by an automated system. The antibiogram was performed according to CLSI recommendations. The colistin MIC was determined by broth micro-dilution according to CA-SFM/EUCAST criteria. The detection of mcr-1 was carried out by immunochromatographic test and PCR mcr-1. Genetic transfers and determination of the plasmid incompatibility group were made. The ST was carried out by MLST using the Pasteur scheme.
The strain has a high-level penicillinase resistance phenotype. It was susceptible to amikacin and resistant to gentamicin, tobramycin, fluoroquinolones, tetracycline, chloramphenicol, and cotrimoxazole. The strain was resistant to colistin (MIC ꞊ 4 μg/ml).
The genetic transfer is given a colistin-resistant transconjugant. The plasmid carrying mcr-1 gene belongs to the incompatibility group IncHI2.The strain belongs to ST19. J Microbiol Infect Dis 2019; 10(4):222-224.
Keywords:
Colistin resistance, mcr-1, Escherichia coli ST19, case report,
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- ISSN: 2146-3158
- Başlangıç: 2011
- Yayıncı: Sağlık Araştırmaları Derneği
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