There is growing interest in the literature concerning the role of employees organizational success. Particularly in hospitality industry becasue of poor working conditions, low wages, long working hours, low career opportunities, employees are faced with job stress and dissatisfaction and consequently these result high turnover rates. Therefore, the ıssues of employee's job stress, satisfaction and turnover intent have gained important in terms of hotel managers for businesses success. The main objective of this study is to determine the relationship between employees' work-related stress, job satisfaction and turnover intent. A survey was used to collect data from 262 five-star hotels' managers and employees in Istanbul. In order to measure the impact of job stress on job satisfaction and turnover intent, correlation and regression analysis were utilized. The findings revealed a negative correlation between job stress and job satisfaction and a positive relationship between job stress and turnover intent.