The present study was carried out in order to explore the effect of different artificial mediums and in vitro storage periods on fertilization, eyeing and hatching success of scaly carp (Cyprinus carpio) eggs. The batches of about 200 pooled eggs treated with 20-ml three different extenders (Ringer, Dettlaff and Cortland) and Ovarian fluid in 15-cm petri dishes, were stored at 22.5⁰ C for 30, 60, or 90 min. The in vitro stored eggs were fertilized by adding of 50 μl sperm which showing motility higher than 80%, in each petri dishes end of the storage period. The highest fertilization rates were determined as 86% and 72% with the egg samples stored for 60 min in Cortland solution and for 90 min in Ovarian fluid respectively (p
Bu çalışma, farklı sulandırıcı ve in vitro muhafaza sürelerinin pullu sazan (Cyprinus carpio) yumurtalarının döllenme, gözlenme ve açılma başarısı üzerine olan etkilerinin araştırılması amacıyla yürütülmüştür. 20-ml hacmindeki üç farklı sulandırıcı (Ringer, Dettlaff ve Cortland) ve ovaryum sıvısı ile 15-cm çaplı petri kutularında muamele edilen yaklaşık 200 yumurta, 22.5⁰ C'de 30, 60 ve 90 dk süre ile muhafaza edildi. İn vitro muhafaza edilen yumurtalar, muhafaza süresinin sonunda %80'den fazla motil özelliğe sahip olan 50 µl sperma ile her petri kutusunda döllenmiştir. En yüksek fertilizasyon oranları, Cortland solüsyonunda 60 dk ve ovaryum sıvısında 90 dk muhafaza edilen yumurta örneklerinde sırasıyla %86 ve %72 olarak belirlenmiştir (p
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Bozkurt Y, Yavaş İ. 2012. Effect of temperatures and storage periods on fertilizing capacity of short-term preserved scaly carp (Cyprinus carpio) eggs. The Israeli Journal of Aquaculture-Bamidgeh. 64:680-685
Carolsfeld J, Godinho HP, Zaniboni Filho E, Harvey BJ. 2003. Cryopreservation of sperm in Brazilian migratory fish conservation. J Fish Biol. 63(2):472-489. doi: 10.1046/j.1095-8649.2003.00170.x
Dettlaff TA, Ginsburg AS, Schmalhausen OI. 1993. Sturgeon fishes: developmental biology and aquaculture. Berlin: Springer-Verlag 299 p. doi: 10.1007/978-3-642-77057-9.
Erdahl AW, Cloud JG, Graham EF. 1987. Fertility of rainbow trout (Salmo gairdneri) gametes: gamete viability in artificial media. Aquaculture. 60(3-4):323-332. doi:10.1016/0044-8486(87)90297-3
Formacion MJ, Hori R, Lam TJ. 1993. Overripening of ovulated eggs in goldfish I. Morphological changes. Aquaculture. 114(1-2):155-168. doi: 10.1016/0044-8486(93)90258-Z
Formacion MJ, Venkatesh B, Tan CH, Lam TJ. 1995. Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids. Fish Physiol Biochem. 14(3):237-246. doi:10.1007/BF00004314
Ginatullina E, Komrakova M, Holtz W. 2018. Chilled storage of unfertilized and fertilized rainbow trout (Oncorhynchus mykiss) eggs in sealed polyethylene bags at different temperatures. Aquaculture. 484:214-218. doi: 10.1016/j.aquaculture.2017.11.028
Goetz FW, Coffman MA. 2000. Storage of unfertilized eggs of rainbow trout (Oncorhynchus mykiss) in artificial media. Aquaculture. 184(3-4):267-276. doi: 10.1016/S0044-8486(99)00327-0
Hobby AC, Pankhurst NW. 1997. Post-ovulatory egg viability in the snapper Pagrus auratus (Sparidae). Mar Freshwater Res. 48(5):385-389. doi: 10.1071/MF96120
Lahnsteiner F, Weismann T. 1999. Changes in eggs of brown trout, rainbow trout and grayling during shortterm storage. N Am J Aquacult. 61(3):213-219. doi: 10.1577/1548-8454(1999)061<0213:CIEOBT>2. 0.CO;2
Linhart O, Barth T, Kouril J, Hamackova J. 1991. Stimulation of spermiation in tench (Tinca tinca L.) by analogues of GNRH and carp hypophysis. Paper presented at: 4 th International Symposium on Reproductive Physiology of Fish; Norwich, UK.
Linhart O, Billard R. 1995. Biology of gametes and artificial reproduction in common tench (Tinca tinca L.). A review. Polskie Arch Hydrobiol. 42(1-2):37-56.
Linhart O, Kudo S, Billard R, Slechta V, Mikodina EV. 1995. Morphology, composition and fertilization of carp eggs: a review. Aquaculture. 129(1-4):75-93. doi: 10.1016/0044-8486(94)00230-L
Niksirat H, Sarvi K, Mojazi Amiri B, Karami M, Hatef A. 2007. In vitro storage of unfertilized ova of endangered Caspian brown trout (Salmo trutta caspius) in artificial media. Anim Reprod Sci. 100(3-4):356-363. doi: 10.1016/j.anireprosci.2006.08.019
Rothbard S, Rubinsthein I, Gelman E. 1996. Storage of common carp (Cyprinuscarpio L.) eggs for short durations. Aquac Res. 27(3):175-181. doi: 10.1111/j.1365-2109.1996.tb00982.x
Samarin AM, Żarski D, Palińska-Żarska K, Krejszeff S, Blecha M, Kucharczyk D, Policar T. 2017. In vitro storage of unfertilized eggs of the Eurasian perch and its effect on egg viability rates and the occurrence of larval malformations. Animal. 11(1):78-83. doi: 10.1017/S1751731116001361
Stoss J. 1983. 6 Fish gamete preservation and spermatozoan physiology. Fish Physiology. 9:305-350. doi: 10.1016/S1546-5098(08)60306-4
Suquet M, Chereguini O, Omnes MH, Rasines I, Normant Y, Souto IP, Quemener L. 1999. Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on shortterm storage capacities of turbot, Psetta maxima, ova. Aquat Living Resour. 12(4):239-246. doi: 10.1016/S0990-7440(00)86634-6
Takano K, Hiroi O, Yasukawa M, Shetake T. 1973. Studies on the retention of gametes of salmonids fishes. I - On the fertility of chum salmon eggs after storage. Scientific Reports of the Hokkaido Salmon Hatchery. 27:31-37.
Tiersch TR. 2011. Process pathways for cryopreservation research, applications and commercialization. In: Tiersch TR, Green CC, editors. Cryopreservation in aquatic species, 2nd edition. Baton Rouge: World Aquacult Society. p. 646-671.
Vuthiphandchai V, Zohar Y. (1999). Age-related sperm quality of captive striped bass Morone saxatilis. J World Aquacult Soc. 30(1):65-72. doi: 10.1111/j.1749-7345.1999.tb00318.x
Wolf K, Quimby MC. 1969. 5 Fish cell and tissue culture. Fish Physiology. 3:253-305. doi: 10.1016/S1546-5098(08)60115-6
Yıldız C, Bozkurt Y, Yavaş İ. (2013). An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm. Cryobiology. 67(1):91-94. doi: 10.1016/j.cryobiol.2013.05.008
Zlabek A, Linhart O. 1987. Short-term storage of nonseminated and unfertilized eggs of the common carp, grass carp and silver carp. Bulletin VURH Vodnany. 23:3-11.