Bazı Aromatik Bitki Esansiyel Yağlarının Patojenik Balık Bakterilerine Karşı Antibakteriyel Aktivitesi
24 adet bitki türünün uçucu yağları hidrodistilasyon yoluyla elde edildi ve 7 çeşit balık patojenine (Aeromonas hydrophila, Aeromonas salmonicida, Vibrio anguillarum, Yersinia ruckeri, Enterococcus faecalis, Lactococcus garvieae ve Streptococcus agalactiae) karşı antibakteriyel etkileri araştırıldı. Uçucu yağların, disk difüzyon yöntemiyle elde edilen antibakteriyel aktivite sonuçları, tüm patojenlere karşı kuvvetli aktiviteleri olduğunu göstermektedir. Genel olarak, Artemisia absinthium dışında tüm uçucu yağlar, balık patojenlerinin çoğunluğuna karşı güçlü antibakteriyel etkiler göstermiştir. Bununla birlikte, A. absinthium uçucu yağı, sadece A. hydrophila’ ya karşı zayıf antibakteriyel etki göstermiştir. Yirmi dört uçucu yağ arasından çoğunlukla yedi uçucu yağ (T. spicata, T. vulgaris, L. nobilis, C. verum, H. plicatum and A. citriodora Paláu) tüm balık patojenlerine karşı iyi antibakteriyel aktivite sergilemişlerdir. Test edilen antibiyotikler (furazolidon, oksitetrasiklin, sefalotin ve trimetoprim/sulfametoksazol) ile karşılaştırıldığında, uçucu yağların antibakteriyal etkileri çoğunlukla eşit veya güçlü olarak bulunmuştur. Uçucu yağların antibakteriyel aktivite sonuçları göz önünde bulundurarak, su ürünleri yetiştiriciliğinde bakteriyel balık hastalıklarına karşı antimikrobiyal ajanların yerine alternatif olarak kullanımları uygun olabilir.
Antibacterial Activity of Some Aromatic Plant Essential Oils Against Fish Pathogenic Bacteria
Essential oils of twenty-four plant species were obtained by hydrodistillation andinvestigated for their antibacterial effects against seven fish pathogenic bacteria(Aeromonas hydrophila, Aeromonas salmonicida, Vibrio anguillarum, Yersiniaruckeri, Enterococcus faecalis, Lactococcus garvieae and Streptococcusagalactiae). The antibacterial activity results of the essential oils obtained by discdiffussion method showed strong activities against all pathogens. In general,whole essential oils except Artemisia absinthium exhibited strong antibacterialeffects against the most of the fish pathogens. However, the essential oil of A.absinthium showed weak antibacterial effect against only A. hydrophila. Mostlyseven essential oils of the plants (T. spicata, T. vulgaris, L. nobilis, C. verum, H.plicatum and A. citriodora Paláu) among twenty-four essential oils exhibited goodantibacterial activity against all fish pathogens. When compared to the testedantibiotics (furazolidon, oxytetracycline, cephalothin, andtrimethoprim/sulfamethoxazole), the antibacterial effects of essential oils weremostly obtained equivalent or stronger. Considering the antibacterial activityresults of the essential oils, their alternative use in lieu of antimicrobial agentsagainst bacterial fish diseases might be convenient in the aquaculture.
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- Adel M, Safari R, Ghitanchi A, Zorriehzahra M. 2016.
Chemical composition and in vitro antimicrobial
activity of some Iranian medical herbs against Yersinia
ruckeri. Iran J Fish Sci. 15(3): 1108-1123.
- Andrews J. 2009. BSAC standardized disc susceptibility
testing method (version 8). J Antimicrob Chemoth.
64(3): 454-489.
doi: 10.1093/jac/dkp244
- Baydar H, Sağdiç O, Özkan G, Karadoğan T. 2004.
Antibacterial activity and composition of essential oils
from Origanum, Thymbra and Satureja species with
commercial importance in Turkey. Food Control.
15(3): 169-172.
doi: 10.1016/S0956-7135(03)00028-8
- Cermelli C, Fabio A, Fabio G, Quaglio P. 2008. Effect of
eucalyptus essential oil on respiratory bacteria and
viruses. Curr Microbiol. 56(1): 89-92.
doi: 10.1007/s00284-007-9045-0
- Conover W. 1999. Practical nonparametric statics. John
Wiley & Sons, Inc., New York. 130-133.
- Görmez Ö, Diler Ö. 2017. Balık Patojenlerine Karşı Bazı
Bitkisel Uçucu Yağların Antibakteriyel Aktivitesi.
Yalvaç Akademi Dergisi. 2(1): 112-122.
- Gurib-Fakim A, Subratty H, Narod F, Govinden-Soulange
J, Mahomoodally F. 2005. Biological activity from
indigenous medicinal plants of Mauritius. Pure Appl
Chem. 77(1): 41-51.
doi: 10.1351/pac200577010041
- Harikrishnan R, Balasundaram C, Heo M-S. 2011. Impact
of plant products on innate and adaptive immune
system of cultured finfish and shellfish. Aquaculture.
317(1): 1-15.
doi: 10.1016/j.aquaculture.2011.03.039
- Kaya I, Yigit N, Benli M. 2008. Antimicrobial activity of
various extracts of Ocimum basilicum L. and
observation of the inhibition effect on bacterial cells by
use of scanning electron microscopy. Afr J Tradit
Complement Altern Med. 5(4): 363-369.
doi: 10.4314/ajtcam.v5i4.31291
- Metin S, Didinen BI, Mercimek EB, Ersoy AT. 2017. Bazı
Bakteriyel Balık Patojenlerine Karşı Bazı Bitkisel
Uçucu Yağlarının Antibakteriyel Aktivitesi.
Aquaculture Studies (Eski Yunus Araştırma Bülteni).
17(1): 59-69.
- Mousavi SM, Wilson G, Raftos D, Mirzargar SS,
Omidbaigi R. 2011. Antibacterial activities of a new
combination of essential oils against marine bacteria.
Aquacult Int. 19(1): 205-214.
doi: 10.1007/s10499-010-9354-3
- Okmen G, Ugur A, Sarac N, Arslan T. 2012. In vivo and
in vitro antibacterial activities of some essential oils of
Lamiaceae species on Aeromonas salmonicida isolates
from cultured rainbow trout, Oncorhynchus mykiss. J
Anim Feed Sci. 11(15): 2762-2768.
doi: 10.3923/javaa.2012.2762.2768
- Olusola S, Emikpe B, Olaifa F. 2013. The potentials of
medicinal plant extracts as bio-antimicrobials in
aquaculture. Int J Med Aromat Plants. 3(3): 404-412.
- Ontas C, Esin B, Kaplaner E. 2016. Antibacterial Activity
of Citrus limon Peel Essential Oil and Argania spinosa
Oil Against Fish Pathogenic Bacteria. Kafkas Üniv Vet
Fak. 22(5): 741-749.
doi: 10.9775/kvfd.2016.15311
- Park H-M, Kim J, Chang K-S, Kim B-S, Yang Y-J, Kim
G-H, Shin S-C, Park I-K. 2011. Larvicidal activity
of Myrtaceae essential oils and their components
against Aedes aegypti, acute toxicity on
Daphnia magna, and aqueous residue. J Med Entomol.
48(2): 405-410.
doi: 10.1603/ME10108
- Randrianarivelo R, Danthu P, Benoit C, Ruez P,
Raherimandimby M, Sarter S. 2010. Novel alternative
to antibiotics in shrimp hatchery: effects of the
essential oil of Cinnamosma fragrans on survival and
bacterial concentration of Penaeus monodon larvae. J
Appl Microbiol. 109(2): 642-650.
doi: 10.1111/j.1365-2672.2010.04694.x
- Rattanachaikunsopon P, Phumkhachorn P. 2007.
Bacteriostatic effect of flavonoids isolated from leaves
of Psidium guajava on fish pathogens. Fitoterapia.
78(6): 434-436.
doi: 10.1016/j.fitote.2007.03.015
- Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P.
2014. Use of plant extracts in fish aquaculture as an
alternative to chemotherapy: current status and future
perspectives. Aquaculture. 433: 50-61.
doi: 10.1016/j.aquaculture.2014.05.048
- Romano L, Battaglia F, Masucci L, Sanguinetti M,
Posteraro B, Plotti G, Zanetti S, Fadda G. 2005. In vitro
activity of bergamot natural essence and furocoumarinfree and distilled extracts, and their associations with
boric acid, against clinical yeast isolates. J Antimicrob
Chemoth. 55(1): 110-114.
doi: 10.1093/jac/dkh503
- Romero J, Feijoó CG, Navarrete P (2012). Antibiotics in
aquaculture-use, abuse and alternatives. Health and
Environment in Aquaculture. In: Carvalho ED DG,
Silva RJ, editors. Rijeka, Croatia, INTECH: pp. 159-
198. p.
- Roomiani L, Soltani M, Akhondzadeh BA, Mahmoodi A,
Taheri MA, Yadollahi F. 2013. Evaluation of the
chemical composition and in vitro antimicrobial
activity of Rosmarinus officinalis, Zataria multiflora,
Anethum graveolens and Eucalyptus globulus against
Streptococcus iniae; the cause of zoonotic disease in
farmed fish. Iran J Fish Sci. 12(3): 702-716.
- Royston P. 1995. Remark AS R94: A remark on algorithm
AS 181: The W-test for normality. Journal of the Royal
Statistical Society. Series C (Applied Statistics). 44(4):
547-551.
doi: 10.2307/2986146
- Shapiro SS, Wilk MB. 1965. An analysis of variance test
for normality (complete samples). Biometrika. 52(3/4):
591-611.
doi: 10.2307/2333709
- Soltani M, Sheikhzadeh N, Ebrahimzadeh-Mousavi H,
Zargar A. 2010. Effects of Zataria multiflora essential
oil on innate immune responses of common carp
(Cyprinus carpio). J Fish Aquat Sci. 5: 191-199.
doi: 10.3923/jfas.2010.191.199
- Türker H, Yıldırım AB. 2015. Screening for antibacterial
activity of some Turkish plants against fish pathogens:
a possible alternative in the treatment of
bacterial infections. Biotechnol Biotec Eq. 29(2): 281-
288.
doi: 10.1080/13102818.2015.1006445
- Van Hai N. 2015. The use of medicinal plants as
immunostimulants in aquaculture: A review.
Aquaculture. 446: 88-96.
doi: 10.1016/j.aquaculture.2015.03.014
- Wang JL, Meng XL, Lu RH, Wu C, Luo YT, Yan X, Li
XJ, Kong XH, Nie GX. 2015. Effects of Rehmannia
glutinosa on growth performance, immunological
parameters and disease resistance to Aeromonas
hydrophila in common carp (Cyprinus carpio L.).
Aquaculture. 435: 293-300.
doi: 10.1016/j.aquaculture.2014.10.004
- Zaouali Y, Bouzaine T, Boussaid M. 2010. Essential oils
composition in two Rosmarinus officinalis L. varieties
and incidence for antimicrobial and antioxidant
activities. Food Chem Toxicol. 48(11): 3144-3152.
doi: 10.1016/j.fct.2010.08.010