Emil WAHDİ, Nisa Rachmania MUBARİK, Rahayu WİDYASTUTİ

Characterization of Phosphate Solubilising Bacteria from Limestone Quarry in Cirebon Indonesia

Limestone quarry has low available P for plant on land revegetation process. Therefore, phosphate-solubilising bacteria could be a solution to dissolve phosphate in limestone in order to add phosphate availability. The research was aimed to characterize and identify the phosphate-solubilising bacteria adapted in limestone quarry. Isolation was performed using Pikovskaya medium which obtained four isolates bacteria had halo-zone around the colony. The ability to dissolve phosphate was examined using five soil samples from different region of limestone quarry. The result showed index of P solubility between 1.63 to 3.42. Identification bacterial isolates by using 16S rRNA gene showed that the isolates have similarity with Pseudomonas mosselii QC5A.1, Pseudomonas mosselii QC5B.1, Pantoea ananatis QC5C.1, and Pseudomonas oryzihabitans QC5B.3.

Keywords:

Limestone quarry, phosphate-solubilising bacteria Pseudomonas, Pantoea, 16S rRNA,

PDF

___

Alia, Afzal A, Khokhar SN, Jabeen B, Asad SA, (2013) Phosphate solubilizing bacteria associated with vegetables roots in different ecologies. Pak. J Bot., 45, 535-544.

Bashan Y, Kamnev AA, de-Bashan LE, (2013) Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure. Biol Fertil Soil., 49, 465-479.

Devereux R, Wilkinson SS, (2004) Amplification of ribosomal RNA sequences. Mol Microb Eco Man (MMEM), 3, 509-522

Galkiewicz JP, Kellogg CA, (2008) Cross kingdom amplification using bacteria spesific primers: complications for studies of coral microbial ecology. Appl Environ Microbiol., 74, 7828-7831.

Gholizadeh A, Ardalan M, Tehrani MM, Hosseini HM, Karimian N, (2009) Solubility test in some phosphate rocks and their potential for direct application in soil. World App Sci J., 6, 182-190.

Godfrey SAC, Harrow SA, Marshall JW, Klena JD, (2001) Characterization by 16S rRNA sequence analysis of Pseudomonads cousing blotch disease of cultivated Agaricus bisporus. Appl. Environ. Microbiol., 67, 4316-4323.

Holt JG, Krieg NR, Sneath PHA, Stanley JT, Williams ST, (1994) Bergey’s Manual of Determinative Bacteriology. 9th Edn., Williams & Wilkins.

Illmer P, Schinner F, (1995) Phosphate solubilizing microorganisms under non-sterile conditions. Page 197-204. Available from: https://diebodenkultur.boku.ac.at/volltexte/band-46/heft-1/illmer.pdf [Accessed 18 August 2014].

International Organisation of Vine and Wine, (2015) Compendium of International Methods of Wine and Must Analysis. 2015th Edn., OIV-18, rue d’Aguesseau 75008 Paris, France.

Jianbo S, Lixing Y, Junling Z, Haigang Li, Zhaohai B, Xinping C, Weifeng Z, Fusuo Z, (2011) Phosphorus dynamics: from soil to plant. Plant Physiol., 156, 997-1005.

Kwon SW, Go SJ, Kang HW, Ryu JC, Jo JK, (1997) Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol., 47(4):1061-1067.

Mursyida E, Mubarik NR, Tjahjoleksono A, (2015) Selection and identification of phosphate-potasium solubilizing bacteria from the area around the limestone mining in Cirebon quarry. Res. J. Microbiol., 10, 270-279.

Nautiyal CS, (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett., 170, 265-270.

Sposito G, (2008) The Chemistry of Soils. Ed ke-2. Oxford (GB): Oxford Univ Pr. Victoria DE, Reyes LL, Benitez ADLC, (2009) Use of 16S rRNA gene for characterization of phosphate-solubilizing bacteria associated with corn. Rev Fitotec Mex.,32, 31-37.