İdiyopatik polihidramniyoz olgularında dinamik tiyol/disülfit homeostazı ve iskemik modifiye albümin seviyeleri

Amaç: Bu çalışmanın amacı, idiyopatik polihidramniosun dinamik tiyol-disülfit homeostazı ve iskemi modifiye albümin düzeyleri ile ilişkisinin olup olmadığını belirlemektir. Gereç ve Yöntemler: Bu prospektif vaka kontrol çalışmasına toplam 126 katılımcı dahil edildi. Hasta grubunu idiyopatik polihidramnios tanısı alan 56 hasta ve kontrol grubunu 56 sağlıklı normal gebe oluşturdu. Native tiyol (-SH), total tiyol (-SH.-SS), dinamik disülfid (-SS), maternal serum değerleri ölçüldü ve gruplar arasında karşılaştırıldı. Bulgular: Çalışmaya 56 idiyopatik polihidramnios ve 56 kontrol grubu olmak üzere 18-35 yaş arası 112 hasta dahil edildi. Maternal serum nativ ve total tiyol değerleri çalışma grubunda kontrol grubuna göre anlamlı derecede yüksekti (382,2 ±78,5 mmol/L - 331,8 ±43,9 mmol/L, p <0,001 ve 435,2 ±76,2 mmol/L - 368,2 ±47,2 mmol/ L, p<0.001). Disülfit/doğal tiyol oranı ve disülfür/toplam tiyol oranı istatistiksel olarak anlamlı derecede yüksek bulundu (7,3 ±2,2'ye karşı 5,5 ±0,9, p<0,001 ve 6,3 ±1,7'ye karşı 5,0 ±0,7, p<0,001) ve doğal tiyol/toplam tiol oranı oranı kontrol grubunda anlamlı olarak daha düşüktü (87,4 ±3,4'e karşılık 90,1 ±1,4, p<0,001). Çalışma grubunda ortalama kordon kanı iskemi modifiye albümin (İMA) 0,69 ±0,02 Absorbans Birimi, kordon kanı nativ tiyol (SH) düzeyi 410,2 ±80,2 ve kordon kanı total tiyol düzeyi 461,1 ±82,1 μmol/l idi. Çalışma grubunun kordon kanı örneklerinde İMA ve Native tiyol/toplam tiyol oranı dışındaki tüm parametreler kontrol grubuna göre daha yüksekti. Sonuç: Kontrol grubu ile karşılaştırıldığında idiyopatik polihidramnios ile komplike olan gebeliklerde tiyol/disülfür dengesi anti-oksidatif duruma doğru kaymıştır.

Anahtar Kelimeler:

İMA, tiyol/ disülfid homeostazı, polihidramnioz

Dynamic thiol/disulphide homeostasis and ischemic modified albumin levels in idiopathic polyhydramnios

Objective: The aim of study was to determine whether idiopathic polyhydramnios is in relation with dynamic thiol-disulfide homeostasis and ischemia modified albumin levels or not. Materials and methods: In this prospective case- control study, a total of 126 participants were included. The patient group consisted of 56 patients who were diagnosed idiopathic polyhydramnios, and the control group consisted of 56 healthy normal pregnant. Native thiol (-SH), total thiol (-SH. -SS), dynamic disulfide (-SS), values from maternal serum were measured and compared between groups. Results: 112 patients aged between 18-35 years, consisting of 56 idiopathic polyhydramnios and 56 control groups were included in the study. Maternal serum native and total thiol values were significantly higher in study group compared to control group (382.2 ±78.5 mmol/L vs. 331.8 ±43.9 mmol/L, p <0.001 and 435.2 ±76.2 mmol/L vs. 368.2 ±47.2 mmol/L, p<0.001). Disulphide / native thiol ratio and disulphide / total thiol ratio ratio was found to be statistically significantly higher (7.3 ±2.2 vs 5.5 ±0.9, p<0.001 and 6.3 ±1.7 vs 5.0 ±0.7, p<0.001), and native thiol/total thiol ratio ratio was significantly lower (87.4 ±3.4 vs 90.1 ±1.4, p<0.001) in control group. Mean cord blood ischemia modified albumin (IMA) was 0.69 ±0.02 Absorbance Unit, cord blood native thiol (SH) level 410.2 ±80.2, and cord blood total thiol level was 461.1 ±82.1 μmol/l in study group. All parameters except IMA and Native thiol / total thiol ratio were higher in cord blood samples of study group compared to control group. Conclusion: The thiol/disulfide balance shifted towards anti-oxidative status in pregnancies complicated with idiopathic polyhydramnios compared to control group.

Keywords:

IMA, Thiol/disulphide homeostasis, polyhydramnios,

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