Fatih AKIN, Abdullah YAZAR, Esra TÜRE, Ümit GÜLTEKİN, Ahmet Osman KILIÇ, Cemile TOPÇU, Dursun ODABAŞ, Alaaddin YORULMAZ

Akut Bronşiyolitli Çocuklarda Paraoksonaz-1 ve Arilesteraz Aktiviteleri

Öz Amaç: Akut bronşiolit iki yaş altı çocuklarda sık görülen bir hastalıktır. Paraoksonaz 1 (PON1) enzimi antioksidan görevi gören bir enzimdir. Arilesteraz (ARES) ana proteinin göstergesi olarak kabul edilmektedir. Bu çalışmanın amacı akut solunum yolu enfeksiyonu olan çocuklarda PON1 ve ARES aktivite düzeylerinin hastalık göstergesi olarak kullanılıp kullanılamayacağını araştırmaktır. Gereç ve Yöntem: Çalışmaya çocuk acil servisine akut bronşiolit nedeniyle başvuran 91 hasta ile yaş ve cinsiyet olarak eşleştirilmiş 39 sağlıklı çocuk dahil edildi. Hastalar Wang skorlama sistemine göre hafif, orta ve şiddetli bronşiolit olarak 3 gruba ayrıldı. Bulgular: Tüm bronşiyolitli hastaların ortalama serum PON1 aktivite düzeyleri 188,05 ± 101,94 U/L, kontrol grubunun ortalaması 302,87 ± 170,52 U/L idi. Tüm bronşiyolitli hastaların ortalama serum ARES aktivite düzeyleri 408,44 ± 109,95 kU/L, kontrol grubunun ortalaması 785,45 ± 168,45 kU/L idi. Akut bronşiyolitli hastalarda ortalama serum PON1 ve ARES aktivite düzeyleri istatistiksel olarak anlamlı derecede düşük bulundu. Hastalık şiddetine göre gruplar arasında istatistiksel olarak anlamlı fark bulunma Sonuç: Akut bronşiyolitli hastalarda PON1 ve ARES aktivite düzeylerinin kontrollere göre daha düşük olduğunu bulduk. Çalışmamızın sonuçları, düşük PON 1 ve ARES aktivite düzeylerinin, akut bronşiyolit patogenezinde rol oynayabileceğini ve oksidatif stresin bronşiolit gelişimi üzerine etkisi olabileceğini göstermektedir.

Anahtar Kelimeler:

akut bronşiolit, arilesteraz( ARES), çocuk, oksidatif stress, paraoxonaz 1 (PON1)

Paraoxonase-1 and Arylesterase Activities in Children with Acute Bronchiolitis

Abstract Aim: Acute bronchiolitis is a disease frequently seen in children under two years of age. The paraoxonase 1 (PON1) enzyme functions as an antioxidant. Arylesterase (ARES) is accepted as the indicator of the main protein. The aim of this study is to investigate whether PON1 and ARES activity levels can be used as an indicator of the disease in children with acute Materials and Methods: Ninety one patients with acute bronchiolitis who atmitted to pediatric emergency unit and 39 age- and sex-matched healthy children were included in the study. Patients were divided into 3 groups as mild, moderate and severe bronchiolitis according to Wang scoring system. Results: The mean serum PON1 activity levels of all patients with bronchiolitis were 188.05 ± 101.94 U/L, and the mean of the control group was 302.87 ± 170.52 U/L. The mean serum ARES activity levels of all patients with bronchiolitis were 408.44 ± 109.95 kU/L, and the mean of the control group was 785.45 ± 168.45 kU/L. Mean serum PON1 and ARES activity levels were found to be statistically significantly lower in patients with acute bronchiolitis. No statistically significant difference was found among groups according to disease severity. Conclusion: We found that PON1 and ARES activity levels were lower in patients with acute bronchiolitis than in controls. The results of our study show that low PON 1 and ARES activity levels may play a role in the pathogenesis of acute bronchiolitis and that oxidative stress may have an effect on the development of bronchiolitis.

Keywords:

acute bronchiolitis, arylesterase (ARES), child, oxidative stress, paraoxonase 1 (PON1),

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