Ramazan YÜKSEL, Fatih EKİCİ, Zeynep SEÇKİN AKKILIK, Didem GÜNERİ, Kadir DEMİRCAN, Sibel SARI, Sevil ARABACI, Gönül GÜROL

Absans epilepsinin genetik sıçan modeli olan WAG/Rij sıçanların beyin dokularında Fetuin-A ekspresyonu

Amaç: Bu çalışmada epilepsinin tanı, tedavi ve prognoz takibinde yeni olası belirteçlerin saptanmasına katkı sağlamak amacı ile absans epilepsili Wistar Albino Glaxo/Rijswijk (WAG/Rij) sıçanlarda beyinin farklı bölgelerinde Fetuin-A düzeylerinin saptanması amaçlandı.Yöntemler: Bu çalışmada genetik absans epilepsili 1, 3 ve 6 aylık erkek WAG/Rij sıçanlar (n=21) kullanıldı. Anestezi altında dekapite edilen tüm sıçanların korteks ve talamus dokuları izole edildi. Gruplar arasındaki Fetuin-A ekspresyonunun düzeyi standart teknikler kullanılarak Western Blot yöntemiyle tayin edildi ve dansiteler arasındaki farklar gruplar arasında karşılaştırıldı.Bulgular: Elde edilen verilere göre; absans epilepsili WAG/Rij sıçanların korteks ve talamus bölgelerinde Fetuin-A ekspresyonu saptanmadı.Sonuç: Bu çalışmada yaşa bağlı gelişim sürecinde absans epilepsili WAG/Rij sıçanların korteks ve talamuslarında Fetuin-A ekspresyonunun olmadığı gösterildi. Elde edilen bulgulardan yola çıkılarak daha ileri moleküler ve histolojik yöntemleri içeren kapsamlı çalışmalar planlanmalı, deneysel olarak elde edilen Fetuin-A bulguları konfirme edilmelidir.

The expression of Fetuin-A in brain tissues of WAG/Rij Rats, genetic rat model of absence epilepsy

Objective: In the present study, we aimed to determine the Fetuin-A levels in different regions of the brain in absence epileptic Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats in order to contribute the identification of new potential biomarkers of the diagnosis, prognosis and follow up the epilepsy treatment.Methods: 1, 3 and 6 months old male WAG/Rij rats (n=21) with absence epilepsy were used in this study. All of the rats were decapitated under anesthesia and their cortex and thalamus tissues were isolated. Fetuin-A levels of the groups were determined by Western Blot method by using standard techniques and differences between densities of the groups were compared.Results: According to data obtained, there was no Fetuin-A expression in brain cortex and thalamus tissues of WAG/Rij rats with absence epilepsy. Conclusion: In this study, it was shown that Fetuin-A is not expressed in brain cortex and thalamus tissues of WAG/Rij rats with absence epilepsy throughout the agerelated development. By evaluating the findings obtained, extensive researches that contain molecular and histological methods must be planned, Fetuin-A findings that are obtained experimentally must be confirmed.

PDF

___

Devinsky O, Vezzani A, Najjar S, et al. Glia and epi- lepsy: excitability and inflammation. Trends Neurosci 2013;36:174-184.
Zupec-Kania BA, Spellman E. An overview of the ke- togenic diet for pediatric epilepsy. Nutr Clin Pract 2009;23:589-596.
Vanlı Yavuz EN, Bebek N. Epilepsi tanı ve tedavisinde elektroensefalografinin (EEG) yeri. Klinik Gelişim 2010;23:35-38.
Sitnikova E, van Luijtelaar G. Cortical and thalamic co- herence during spike-wave seizures in WAG/Rij rats. Epilepsy Res 2006;71:159-180.
Sitnikova E, van Luijtelaar G. Electroencephalo- graphic characterization of spike-wave discharges in cortex and thalamus in WAG/Rij rats. Epilepsia 2007;48:2296-2311.
Coenen AM, van Luijtelaar EL. Genetic animal models for absence epilepsy: a review of the WAG/Rij strain of rats. Behav Genet 2003;33:635-655.
Mori K, Emoto M, Inaba M. Fetuin-A and the cardiovas- cular system. Adv Clin Chem 2012; 56: 175-195.
Ma S, He Z, Zhao J, et al. Association of AHSG gene polymorphisms with ischemic stroke in a Han Chinese population. Biochem Genet 2013;51:916-926.
Dziegielewska KM, Mollgard K, Reynolds ML, Saun- ders NR. A Fetuin-related glycoprotein (alpha 2HS) in human embryonic and fetal development. Cell Tissue Res 1987;248:33-41.
Demircan K, Topcu V, Takigawa T, et al. ADAMTS4 and ADAMTS5 knockout mice are protected from ver- sican but not aggrecan or brevican proteolysis during spinal cord injury. Biomed Res Int 2014;2014:693746.
Patel HC, Ross FM, Heenan LE, et al. Neurodegener- ative actions of interleukin-1 in the rat brain are medi- ated through increases in seizure activity. J Neurosci Res 2006;83:385-391.
Akin D, Ravizza T, Maroso M, et al. IL-1? is induced in reactive astrocytes in the somatosensory cortex of rats with genetic absence epilepsy at the onset of spike-and-wave discharges, and contributes to their occurrence. Neurobiol Dis 2011;44:259-269.
Choi J, Koh S. Role of brain Inflammation in epilepto- genesis. Yonsei Med J 2008;49: 1-18.
Vezzani A. Brain inflammation and seizures. Epilepsy Curr 2004;4:73-75.
Shandra AA, Godlevsky LS, Vastyanov RS, et al. The role of TNF-alpha in amygdala kindled rats. Neurosci Res 2002;42:147-153.
Eriksson C, Zou LP, Ahlenius S, et al. Inhibition of kainic acid induced expression of interleukin-1 beta and interleukin-1 receptor antagonist mRNA in the rat brain by NMDA receptor antagonists. Brain Res Mol Brain Res 2000;85:103-113.
Bambal G, Çakıl D, Ekici F. Models of experimental epilepsy J Clin Exp Invest 2011;2:118-123.
Luijtelaar G, Lyashenko S, Vastyanov R, et al. Cyto- kines and absence seizures in a genetic rat model. Neurophysiology 2012;43:478-486.
Bilgir F, Bilgir O, Kebapcilar L, et al. Soluble CD40 ligand, high sensitive C-reactive protein and fetuin- A levels in patients with essential thrombocythemia. Transfus Apher Sci 2012;46:67-71.
Lim P, Moutereau S, Simon T, et al. Usefulness of fetuin-A and C-reactive protein concentrations for prediction of outcome in acute coronary syndromes (from the French Registry of Acute ST-Elevation Non- ST-Elevation Myocardial Infarction [FAST-MI]). Am J Cardiol 2013;111:31-37.
Smith ER, Nilforooshan R, Weaving G, Tabet N. Plas- ma fetuin-A is associated with the severity of cognitive impairment in mild-to-moderate Alzheimer's disease. J Alzheimers Dis 2011;24:327-333.
Daveau M, Davrinche C, Djelassi N, et al. Partial hep- atectomy and mediators of inflammation decrease the expression of liver alpha 2-HS glycoprotein gene in rats. FEBS Lett 1990;273:79-81.
Daveau M, Christian-Davrinche, Julen N, et al. The synthesis of human alpha-2-HS glycoprotein is down- regulated by cytokines in hepatoma HepG2 cells. FEBS Lett 1988;241:191-194.
Harris VK, Donelan N, Yan QJ, et al. Cerebrospinal fluid fetuin-A is a biomarker of active multiple sclero- sis. Mult Scler 2013;19:1462-1472.
Ottervald J, Franzén B, Nilsson K, et al. Multiple scle- rosis: Identification and clinical evaluation of novel CSF biomarkers. J Proteomics 2010;73:1117-1132.
Puchades M, Hansson SF, Nilsson CL, et al. Pro- teomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease. Brain Res Mol Brain Res 2003;118:140-146.