GEHLOT Hukam S., Narpat S. SHEKHAWAT, Anila PUROHİT

Metabolic changes and protein patterns associated with adaptation to salinity in Sesamum inicum cultivars

Sesamum inducum, yarı kurak bölgelerin önemli yağlı tohumlu bitkisidir. Son yıllarda, S. indicum kültürlerinin sodyum klorür (NaCl) tuzuna olan nispi frekansları değerlendirildi. Proteinlerin ve diğer metabolitlerin elektroforetik profillerinde tuzluluğun uyardığı değişiklikler sodyum klorürün farklı konsantrasyonlarında çimlenen ve büyüyen tohumlarında kaydedildi (0,30, 50 ve 70 mM). NaCl ün 30 mM dan daha yüksek konsantrasyonlarda cv.RT-125 in fide büyümesi gecikti. Cv. Rt-46, RT-54 ve RT-127 lerin kontrol fideleri, cv. RT-125 ile kıyaslandığında toplam soluble şekerlerin ve sakkarozun yüksek seviyeleri gözlendi, cv. RT-127 kontrol fidelerinin prolin içerikleri diğer kültürlerle karşılaştırıldığında düşük bulundu. Soluble protein seviyeleri, tüm deneysel koşullarda RT-125 ve RT-127 de RT-46 ve RT-54 dekinden daha yüksekti. Bununla beraber şekerlerin düzeyi tuz uygulanmış cvs. RT-127, RT-46 ve RT-54 kültürler RT-125 kıyaslandığında daha fazla bulundu. Tuza duyarlı kültürlerin (RT-125) diğerlerine oranla daha fazla malondialdehid (MDA) ve prolin biriktirdikleri belirlendi. Diğer taraftan tuza toleranslı kültürler RT-54,.RT-46 ve RT-127 kültürleri RT-125 ile kıyaslandığında, yüksek süperoksit-dismutaz (SOD) ve düşük MDA aktivitesi gözlendi.

Sasemum indicum kültürlerinde tuzluluğa adaptasyonla ilişkili protein profilleri ve metabolik değişmeler

Sesamum indicum is an important oil seed crop of semi-arid regions. Recently released cultivars of S. indicum were evaluated for their relative tolerance to sodium chloride (NaCl) salt. Salinity induced alterations in electrophoretic patterns of proteins and other metabolites were recorded in seeds germinated and grown in presence of different concentrations of sodium chloride (0, 30, 50 and 70mM). Seedling growth was retarded in cv. RT-125 in presence of NaCl more than 30mM concentration. Control seedlings of cv. RT-46, RT-54 and RT-127 showed higher levels of total soluble sugars and sucrose as compared to cv. RT-125. Proline contents of control seedlings of cv. RT-127 was low as compared to other cultivars. Levels of soluble proteins were higher in RT-125 and RT-127 than in RT-46 and RT-54 under all experimental conditions. However levels of sugars were higher in salt-treated cvs.RT-127, RT-46 and RT-54 as compared to RT-125. Salt sensitive cultivar (RT-125), accumulated higher contents of malondialdehyde (MDA) and proline as compared to the others. On the other hand salt tolerant cultivars RT-54, RT-46 and RT-127 showed higher activity of superoxide-dismutase (SOD) and low MDA content as compared to RT-125.

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