Cytogenetic effect of heavy-metal and cyanide in contaminated waters from the region of southwest Bulgaria

Panagjurishte-Güneybatı Bulgaristan bölgesinde suyu kirleten ağır metal ve siyanürün sitogenetik etkileri çalışılmıştır. In vivo Allium cepa'da bitki test sistemi kullanılmıştır. Sonuç olarak, hücre bölünme hızında bir azalma ve kromozom mutasyonlarından kaynaklanan normal mitoz bölünmeden sapmalar tespit edilmiştir. Kullanılan metod, ağır metaller ve siyanür ile kontamine olmuş suyun biyolojik olarak görüntülenmesi için uygulanabilir bir metodtur.

Bulgaristan' ın güneybatı bölgesindeki kontamine sularda ağır metal ve seyanürün sitogenetik etkileri

A study was made on the cytogenetic effect of heavy-metal and cyanide polluted waters from the region of Panagjurishte - Southwest Bulgaria. A plant test-system Allium cepa in vivo was used. A decreased cell division rate was established, and deviations from the normal mitosis in the result of chromosome mutations were registered. The method used is applicable for biological monitoring of waters contaminated with heavy metals and cyanides.

___

  • Ananoshtev N, Velcheva I, Ivanova E, Staikova T and Yambolieva Z. Risk factors for the increased frequency of malignant tumour formations in a region of South Bulgaria subjected to anthropogenic pressure. Nauchni trudove na PU “P. Hilendarski”. Plantarum. 6: 89-94, 2001.
  • Boyadjiev V, Alexieva Ts, Zaprjanov Z, Hadjieva Y, Koleva M, Tzacheva N, Boyadjieva P and Nikolova P. Heavy metal poisonings. Medicina i fizkultura. Sofia. 242,1990.
  • Bruning T and Chronz C. Occurrence of urinary tract tumors in miners highly exposed to dinitroluene. YOEM. 3: 144-149, 1999.
  • Chang L, Meier J and Smith M. Application of plant and earthworm bioassays to evaluate remediation of a leadcontaminated soil. Arch. Environ. Contam. Toxicol. 32:166-171, 1997.
  • Chernozemski I and Shishkov T. Oncology, SIELA-SOFT.Sofia. 15-21, 2001.
  • Dimitrova I. Studies of grass plants under conditions of industrial pollution with heavy metals and sulphur dioxide. Author’s summary of Ph.D. Dissertation, “P. Hilendarski” Plovdiv University, 1993.
  • Dimitrova I and Ivanova E. Effect of heavy metal soil pollution on some morphological and cytogenetical characteristuics of flax (Linum usitatissum L.). J. of Balkan Ecology. 4: 212-218, 2003.
  • Dimov S and Hristov S. Analysis of the ecological status and the opportunities for sustainable development of mining regions. Minno Delo i Geologija. 1: 28-32, 1998.
  • Fiskesjö G. The Allium test as a standard in environmental monitoring. Hereditas. 102: 99 – 112, 1985.
  • Fiskesjö G. The Allium test – an alternative in environmental studies: the relative toxicity of metal ions. Mutation Research. 197: 243 – 260, 1988.
  • Fiskesjö G. Allium test. Methods in molecular biology. In vitro toxicity testing protocols. 43: 119 – 127, 1995.
  • Fiskesjö G. Allium test for screening chemicals; evaluation of cytological parameters. Plants for environmental studies. CRC Press LLC – New York. p. 308 – 333.1997.
  • Hoga O, Bose S and Sinha S. Vitamin C mediated minimization of Malathion and Rogor induced mitoinhibition and clastogeny. Cytology. 3: 389-397, 1991.
  • Ivanova E, Staikova T and Velcheva I. Mutagenic effect of water polluted with heavy metals and cyanides on Pisum sativum plant in vivo. J. Balkan Ecology. 3: 307-310, 2002.
  • Kovalchuk O, Titov V, Hohn B and Kovalchuk I. A sensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat. Biotechnol. 6: 568-572, 2001.
  • Mecheva R, Karapetkova M and Gerasimov S. Heavy metal accumulation in bioindicator vertebrates in the region of Srednogorie. In: First National Conference on the Problems of Biological Monitoring. Inst. Zool. BAS.Sofia p. 1987.
  • Mitrov G and Chernozemski I. Nutrition and Cancer. Medicina i Fizkultura. Sofia. 67-98, 1985.
  • Moraes D and Jordao B. Evaluation of the genotoxic potential of municipal waste water discharged into the Paraguay River during periods of flood and drought. Environ. Toxicol. 16: 113-116, 2001.
  • Nikolov H. The chemical mutagens as environmental pollutants. Genetika i Selekcija. 1: 77-84, 1987.
  • Ormrod D. Effect of trace element contamination of plants. In: Treshow M. ed., Hidrometeoizdat, Leningrad, Air Pollution and Plant Life. p. 327-356, 1988.
  • Ordinance No 7, State Newspaper, 1986, No 96. Rank J and Nielsen M. Genotoxicity testing of waste water sludge using the Allium cepa anaphase-telophase chromosome aberration assay. Mutat. Res. 418: 113-119, 1998.
  • Sabti K Allium test for air and water borne pollution control. Cytobios. 58: 71-78, 1989.
  • Smaka-Kinkl V, Stegnar P, Lovka M and Toman M. The evaluation of waste, surface and ground water quality using the Allium test procedure. Mutat. Res. 368: 171-179, 1996.
  • Tzonevski D, Sapundzhiev K and Vodenicharov E. Studying the contents of lead and cadmium in the blood of children up to 15 years old from the region of the Non- Ferrous-Metal Works near Plovdiv. Higiena i Zdraveopazvane. 2/3: 20-22, 1998.
  • Vodenicharska TZ, Petrov I and Razboinikova F. A study for heavy metal (lead, cadmium, zinc, copper, manganese) contamination among the population from the 3rd Metallurgical Base region. Higiena i Zdraveopazvane. 2: 59-62, 1992.