Mehmet TOPÇUL, Seyhan ALTUN, Özcan Gül ARICAN

Inhibitory effect of 57% hepatectomized mice serum on the growth of L-cells

Erişkin memeli karaciğer hücreleri, hücre siklusunun G0 fazında olup, belli fonksiyonları yapmak üzere farklılaşmışlardır ve bölünmezler. Ancak, yaralanma veya parsiyal hepatektomi (PH) sonucu, hücreler hızlı bir şekilde çoğalarak organın rejenere olmasını sağlarlar. Daha önceki bir çalışmada, %35 PH yapılan farelerden elde edilen serumun, L-hücrelerinin büyümesini dördüncü günden sonra inhibe ettiği tesbit edilmiştir. Bu çalışmada ise, %57 hepatektomi uygulanan farelerden serum elde edilmiş ve bu serum L-hücrelerinin yaşama ortamlarına ilave edilmiştir. Farklı günlerde hücreler toplanarak, canlı hücre sayıları ve işaretlenme indeksleri saptanmıştır. Elde edilen sonuçlara göre, %57 PH uygulanan hayvanlardan elde edilen serumun L-hücrelerinin büyümesi üzerinde ilk günlerde inhibisyon etkisi meydana getirdiği tesbit edilmiştir.

%57 hepatektomi uygulanmış fare serumunun L-hücrelerinin çoğalmasını baskılayıcı etkisi

Adult mammalian liver cells, which are in cell cycle’s G0 phase, differentiate to accomplish specific functions and do not divide. Only as a result of damage or partial hepatectomy (PH), the cells multiply in a rapid way and cause the organ’s regeneration. In one of our previous studies, we observed that sera obtained from 35% partially hepatectomized mice has an inhibitory effect on the growth of the cells after the fourth day. In this study, sera are obtained from 57% hepatectomized mice and added to medium of L-cells. In the different days, viable cell number and labelling index were investigated. According to the results obtained from the experiments it was determined that sera obtained from 57% partially hepatectomized mice has an inhibitory effect on the growth of the L-cells in early days.

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