Efficiency of the gamma irradiation in the induction of in vitro somatic mutations

Bu çalışmaya şeker pancarı (Beta vulgaris L.) bitkisinde somatik mutasyonları teşvik etmek amacı ile başlandı. E-145 şeker pancarı hattına ait meristem kültürleri kuruldu ve bu kültürler 0, 10, 25, 50, 75 ve 100 Gy lik gama radyasyonu ile ışınlandı. Işınlanmış meristem kültürlerinden rejenere olan bitkilerin ortalama taze ve kuru ağırlıkları tespit edildi. Bu sonuçlara göre GR50 dozu saptandı. E-145 hattı için optimum doz olarak 20 Gy belirlendi. E-145 şeker pancarı hattına ait bitkilerin döllenmemiş ovüllerinden in vitro koşullarda elde edilen haploid şeker pancarları bu dozla (20 Gy) ışınlandı. Işınlanmış haploid bitkiler vejetatif olarak çoğaltıldı ve M1V1-M1V3 generasyonları elde edildi. Bu generasyonlarda köklenme yeteneklerine göre mutant bitkiler belirlendi.

In vitro somatik mutasyon oluşturulmasında gama radyasyonunun etkisi

This study has been performed to induce somatic mutations in sugar beet (Beta vulgaris L.). Meristem cultures of sugar beet (line E-145) were established and irradiated with 0, 10, 25, 50, 75 and 100 Gy of gamma radiation. In irradiated cultures, average fresh and dry weights of the regenerated plants were determined. From the results obtained, GRso value was calculated. For the E-145 line, 20 Gy was defined as the optimum dosage. At first, the haploid sugar beet plants (line E-145) that were obtained by culturing unfertilized ovules were irradiated with this dosage (20 Gy). Irradiated haploid plants were vegetatively reproduced and M1V1-M1V3 generations were obtained. Mutant plants in these generations were then determined according to their rooting capabilities.

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