Evgeniya N. IVANOVA, Iliana G. VELCHEVA, Teodora A. STAIKOVA

Cytogenetic testing of heavy metal and cyanide contaminated river waters in a mining region of southwest Bulgaria

Ağır metal ve siyanid ile kirlenmiş nehir suyu üzerindeki sitogenetik bu araştırma, mikronukleus ve anafaz analizleri kullanılarak, mitotik ve faz indekslerinin hesaplanması ile Güneybatı Bulgaristan'ın Panagjurishte bölgesinde yürütüldü. In vivo deney materyali olarak Allium cepa kullanıldı. Elde edilen veriler hücre çoğalmasında bir azalma ve normal mitoz-mikronukleus, anafaz ve telofaz köprüleri ile fragmentler, geç kalan kromozomlar ve bir C-mitotik etkisinin meydana geldiğini gösterdi. Sitogenetik analizler ağır metal ve siyanid ile kirlenmiş suların biyolojik görüntülenmesinin etkili ve uygun olduğunu kanıtlamak için yapıldı.

Güneybatı Bulgaristan' daki maden bölgesinde ağır metal ve siyanid ile kirlenmiş nehir suyunun sitogenetik analizi

Using micronuclei and anaphase analyses and through calculating mitotic and phase indices, a cytogenetic study was conducted on heavy metal- and cyanide contaminated river waters from the region of Panagjurishte - Southwest Bulgaria. Allium cepa was used as a test-object in vivo. The data obtained showed a decreased cell reproduction and the presence of deviations from the normal mitosis - micronuclei, anaphase- and telophase bridges and fragments, lagging chromosomes and a C-mitotic effect: The cytogenetic analyses used proved to be effective and suitable for biomonitoring of heavy metal- and cyanide contaminated waters

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Ananoshtev N, Velcheva I, Ivanova E, Staikova T and Yambolieva Z. Risk factors for the increased frequency of malignant tumor formations in a region of South Bulgaria subjected to anthropogenic pressure. Nauchni trudove na PU “P. Hilendarski”, Plantarum. 6: 89-94, 2001.
Boyadjiev V, Alexieva Ts V, Zaprjanov ZK, Hadzhieva YT, Koleva MI, Tzacheva NK, Boyadjieva PV and Nikolova PO. Heavy metal poisonings. Medicina i fizkultura. Sofia 1990.
Bruning T and Chronz C. Occurrence of Urinary Tract Tumors in Miners Highly Exposed to Dinitroluene. YOEM. 3: 144-149, 1999.
Chang L, Meier J, Smith M. Application of plant and earthworm bioassays to evaluate remediation of a leadcontaminated soil. Arch. Environ. Contam. Toxicol. 32: 166-171, 1997.
Chernozemski I and Shipkov T. Oncology. SIELA-SOFT. Sofia. 2001.
Cotelle S, Masfaraud JF and Ferard GF. Assessment of the genotoxicity of contaminated soil with the Allium/Viciamicronucleus and the Tradescantia –micronucleus assays. Mutation. Research. 426: 167-171, 1999.
Dimitrova I. Studies of grass plants under conditions of industrial pollution with heavy metals and sulfur dioxide. Author’s summary of Ph.D. Dissertation, “P. Hilendarski”. Plovdiv University. 1993.
Dimitrova I and Ivanova E. Effect of heavy metal-soil pollution on some morphological and cytogenetical characteristics of flax (Linum usitatissum). Journal of Balkan Ecology. 6: 212-218, 2003.
Dimov S and Hristov S. Analysis of the ecological status and the opportunities for sustainable development of mining regions. Minno delo i geologija. 1: 28-32, 1998.
Fenech M. The cytokinesis-block micronucleus assay in nucleated cells. In: Mutation and the Environment, Part B, Mendelsohn ML, Albertini RJ, Eds., Wiley-Liss, New York. 195-206, 1990.
Fiskesjo G. The Allium test as a standart in environmental monitoring. Hereditas.102: 99-112, 1985.
Fiskesjo G. The Allium test – a potential standard for the assessment of environmental toxicity. In: Environmental Toxicology and Risk Assessment. Gorsuch/Dwyer/Ingersoll/LaPoint. ASTM, Philadelphia, PA. 331-345, 1993.
Fiskesjo G. The Allium test in Wastewater Monitoring. An International Journal of Environmental Toxicology and Water Quality. 8: 291-298, 1993.
Fiskesjo G. Allium test II: assessment of a Chemical’s Genotoxic Potential by Recording Aberrations in Chromosomes and Cell Division in Root Tips of Allium cepa L. An International Journal of Environmental Toxicology and Water Quality. 9: 235-241, 1994.
Fiskesjo G. Allium test for screening chemicals; evaluation of cytological parameters. In: Plants for Environmental Studies. Lewis Publisher. Boca Raton, New York. 307-333, 1997.
Ivanova E, Staikova T and Velcheva I. Mutagenic effect of water polluted with heavy metals and cyanides on Pisum sativum plant in vivo. Journal of Balkan Ecology. 3: 307-310, 2002.
Kovalchuk O, Titov V, Hohn B and Kovalchuk I. Asensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat. Biotechnol. 6: 568-572, 2001.
Ma T-H, Xu J, Xia W, Jong X, Sun Wand Lin G. Proficiency of the Tradescantia – micronucleus image analysis system for scoring micronucleus frequencies and data analysis. Mutat. Res. 270: 39-44, 1992.
Mecheva R, Karapetkova M and Gerasimov S. Heavy metal accumulation in bioindicator vertebrates in the region of Srednogorie. In: First National Conference on the Problems of Biological Monitoring. 1987. Zool., BAS, Sofia. 1987.
Mitrov G and Chernozemski I. Nutrition and cancer. Medicina i fizkultura, Sofia. 1985.
Moraes D and Jordao B. Evaluation of the genotoxic potential of municipal waste water discharged into the Paraguay River during periods of food and drought. Environ. Toxicol. 16: 113-116, 2001.
Ordinance Nº7. State Newspaper. No 96. 1986.
Rank J and Nielsen M. Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome abberation assay. Mutat. Res. 418: 113-119, 1998.
Sabti K. Allium test for air and water borne pollution control. Cytobios. 58: 71-78, 1989.
Schmid W. The micronucleus test. Mutat. Res. 31: 9-15,1975
Smaka-Kinkl V, Stegnar P, Lovka M and Toman M. The evaluation of waste, surface and ground water quality Cytogenetic testing of heavy metal and cyanide 105 using the Allium test procedure. Mutat. Res. 368: 171-179, 1996.
Tzonevski D, Sapundzhiev K and Vodenicharov E. Studying the contents of lead and cadmium in the blood of children up to 15 years old from the region of the Non- Ferrous-Metal Works near Plovdiv. Higiena i zdraveopazvane, 2/3: 20-22, 1998.
Vodenicharska Ts, Petrov I and Razboinikova F. A study for heavy metal (lead, cadmium, zinc, copper, manganese) contamination among the population from the 3rd Metallurgical Bbase region. Higiena i zdraveopazvane. 2: 59-62, 1992.