Primula veris L. is a well known pharmaceutical plant, what for it is widely collected in all SE Europe, particularly in Kosovo. Our aim is to determine a molecular polymorphism of its populations from Kosovo. Leaves extracted DNA was investigated in details for presence of polymorphism. RAPD analyses were conducted using 20 different short primers. Genomic DNA amplification profiles were analyzed and processed using data labelling. Comparison between cowslip populations in genetic composition revealed that samples from Bogaj were too distinct on their own. Analyzed molecular variation was observed to have more molecular variation within populations (73%), compared to among populations (27%). Highest genetic distance is observed between Leqinat and M. e Madhe. He values were highest in Bogaj population, while lowest in M. e Madhe population. Results indicated that Bogaj population are more polymorphic. RAPD allows identifying and assessing genetic similarities among plant populations