Evaluation of Antibacterial Activity of PHY-ZnONPs against Certain Multi-Drug Resistant (MDR) Bacteria

In recent years, due to the increasing drug resistance and toxicity of many existing drugs the use of bioactive compound to overcome the pathogens has been highly regarded. Phycocyanin is one of the important biocompatible compounds with the significant antibacterial effects. It is the major light-harvesting pigment in cyanobacteria. On the other hand, nanoparticles are a new area to fight the infections. The biogenic synthesis of ZnO nanoparticles using capping potential of bioactive compounds can be a novel strategy to enhance their antibacterial activities. Considering this goal, the antibacterial and antibiofilm activity of phycocyanin-Zinc Oxide nanoparticles (PHY-ZnONPs) was investigated for the first time. Phycocyanin pigment was isolated from a native cyanobacterial strain, Limnothrix sp. KO05. In the following, the manufacturing functionalized phycocyanin, PHY-ZnONPs were synthesized. The antibacterial effect of PHY-ZnONPs evaluated on selected clinical Gram negative ESBL (extended spectrum beta lactamases)-producing E. coli, ESBL Pseudomonas aeruginosa and Gram positive methicillin-resistant bacterium Staphylococcus aureus (MRSA). The effect of PHY-ZnONPs on extracellular polysaccharides (EPS) production by tested clinical isolates showed a definite order of inhibitory effect as follow: E. coli > S. aureus > P. aeruginosa. PHY-ZnONPs with a high effect against selected clinical MDR isolates can be envisaged as a prospective nanoantibiotic for inhibiting of biofilm formation and bacterial virulence.

Keywords:

phycocyanin, nanoparticles, antibacterial activity natural compounds,

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Srivastava AK, Rai NR, Neilan BA. 2013. Stress biology of cyanobacteria: Molecular mechanisms to cellular responses. CRC Press. 3-28.
Sabarinathan KG, Ganesan G. 2008. Antibacterial and toxicity evaluation of C-phycocyanin and cell extract of filamentous freshwater cyanobacterium-Westiellopsis sps. European Review for Medical and Pharmacological Sciences. 12(2): 79-82.
Sitohy M, Osman A, Abdel-Ghany AG, Salama A. 2015. Antibacterial phycocyanin from Anabaena oryzae SOS13. International Journal of Applied Research in Natural Products. 8(4): 27-36.
Fuente-Nunez C, Reffuveille F, Fernandez L, Hancock RE. Bacterial biofilm development as a multicellular adaptation: anti-biotic resistance and new therapeutic strategies. Current Opinion in Microbiology. 16(5): 580-589.
Frieden T. 2013. Antibiotics resistance threats. Centers for Disease Control and Prevention.
Huh AJ, Kwon YJ. 2011. “Nanoantibiotics”: A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. Journal of Controlled Release. 156(2): 128-145.
De-Jong WH, JA-Borm P. 2008. Drug delivery and nanoparticles: Applications and hazards. International Journal of Nanomedicine. 3(2): 133-149.
Alia K, Dwivedi S, Azam A, Saquib Q, Al-Said MS, Alkhedhairy AA, Musarrat J. 2016. Aloe vera extract functionalized zinc oxide nanoparticles as nanoantibiotics against multi-drug resistant clinical bacterial isolates. Journal of Colloid and Interface Science. 472: 145-156.
Dobrucka R, Dlugaszewska J. 2016. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. Saudi Journal of Biological Sciences. 23(4): 517-523.
Haghighi O, Shahryari S, Ebadi M, Modiri S, Zahiri SH, Maleki H, Akbari-Noghabi K. 2017. Limnothrix sp. KO05: A newly characterized cyanobacterial biosorbent for cadmium removal: the enzymatic and non-enzymatic antioxidant reactions to cadmium toxicity. Environmental Toxicology and Pharmacology. 51: 142-155.
Gantar M, Simovic D, Djilas S, Gonzalez WW, Miksovska J. 2012. Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp. strain 37-2-1. Journal of Biotechnology. 159(1-2): 21-26.
Luo X, Smith P, Raston CL, Zhang W. 2016. Vortex fluidic device-intensified Aaqueous two phase extraction of C-Phycocyanin from Spirulina maxima. ACS Sustainable Chemistry & Engineering. 4(7): 3905-3911. [13] Villanova PA. 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI USA. 32(2): 16-18.
Dwivedi S, Wahab R, Khan F, Mishra YK, Musarrat J, Al-Khedhairy AA. 2014. Reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination. PLOS one. 9(11): e111289.
Gurunathan S, Han JW, Kwon DN, Kim JH. 2014. Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against gram-negative and gram-positive bacteria. Nanoscale Research Letters. 9(1): 373-389.
Khan ST, Ahmed M, Alhadlaq HA, Musarrat J, Al-Khadhairy AA. 2013. Comparative effectiveness of NiCl2, Ni- and NiO-NPs in controlling oral bacterial growth and biofilm formation on oral surfacesArchives of Oral Biology. 58(12): 1804-1811.
Chua SL, Yam JK, Hao P, Adav SS, Salido MM, Liu Y, Givskov M, Sze SK, Tolker-Nielsen T, Yang L. 2016. Selective labeling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms. Nature Communications. 7: 10750.
Dosler S, Karaaslan E. 2014. Inhibition and destruction of Pseudomonas aeruginosa biofilms by antibiotics and antimicrobial peptides. Peptides. 62: 32–37.
Meers P, Neville M, Malinin V, Scotto AW, Sardaryan G, Kurumunda R, Mackinson C, James G, Fisher S, Perkins WR. 2008. Biofilm penetration, triggered release and in vivo activity of inhaled liposomal amikacin in chronic Pseudomonas aeruginosa lung infections. Journal of Antimicrobial Chemotherapy. 61(4): 859-868.
Sawai J, Yoshikawa T. 2004. Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay. Journal of Applied Microbiology. 96(4): 803-809.
Bhande RM, Khobragade CN, Mane RS, Bhande S. 2013. Enhanced synergism of antibiotics with zinc oxide nanoparticles against extended spectrum β-lactamase producers implicated in urinary tract infections. Journal of Nanoparticle Research. 15(1): 1413.
Banoee M, Seif S, Nazari ZE, Jafari-Fesharaki P, Shahverdi HR, Moballegh A, Moghaddam KM, Shahverdi AR. 2010. ZnO nanoparticles enhanced antibacterial activity of ciprofloxacin against Staphylococcus aureus and Escherichia coli. Journal of Biomedical Materials Research Part B. 93(2): 557-561.