Muhamad ALI, İne KARNI, Muhamad AMIN, Muhammad ICHSAN

Development of Growth Media for Bacillus amyloliquefaciens subsp. plantarum, a Poultry Probiotic Candidate

Bacillus amiloliquefaciens subsp. plantarum is a poultry probiotic candidate due to its ability to produce three importantdigestive enzymes; protease, amylase, and cellulase. In its application, the bacterial isolate needs to be cultivated in massscale, therefore requires a lot of growth medium. Thus, this study aimed at developing low-cost media to replace expensivecommercial growth media for culturing the probiont. Three media (two media developed by using two local sources) and onecommercial LB medium as the control were used to culture B. amiloliquefaciens subsp. plantarum. Growth was measured interms of Optical Density (OD), and total harvested bacterial biomass (mg). The results indicated that there was no significantdifference in OD of the probiont measured at the three media, which indicates that both local source-based media could supportthe growth of the probiont at the same rate as the commercial medium. Furthermore, total biomass of the probiont harvestedfrom palm sugar medium was significantly higher (12.130 mg/mL), compared to either tofu wastewater (10.870 mg/mL),or LB medium (7.800 mg/mL). This result suggests that palm sugar medium was the most suitable medium for culturingB. amyloliquefaciens subsp. plantarum on a mass scale. However, the quantity and activity of enzyme synthesized by thebacterium cultured in the local source medium should be further investigated.

Keywords:

Bacillus amyloliquefaciens LB media, palm sugar, tofu wastewater, growth,

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Krishnamoorthy, U., et al., Nitrogen fractions in selected feedstuffs. Journal of Dairy Science, 1982. 65(2): p. 217-225.
Natalia, G., D.H. Darwanto, and S. Hartono, Analysis of Soybean Availability in Indonesia. Ilmu Pertanian (Agricultural Science), 2017. 2(1): p. 042-047.
Van Immerseel, F., et al., Beneficial microbial signals from alternative feed ingredients: a way to improve sustainability of broiler production? Microbial biotechnology, 2017. 10(5): p. 1008-1011.
Buchanan, R. and N. Gibbons, Bergey’s manual of systematic bacteriology. William and Wilkens, 1984.
Wizna, H.A., et al., Improving the quality of tapioca by-products (onggok) as poultry feed through fermentation by Bacillus amyloliquefaciens. Pakistan Journal of Nutrition, 2009. 8(10): p. 1636-1640.
Atlas, R.M., Handbook of microbiological media. 2010: CRC press.
https://www.nutritionix.com/food/palm-sugar. [accessed September 23rd 2017].
http://ridwanaz.com/kesehatan/mengenal-gula50 M. Ali et al. / JABS, 12 (1): 46-50, 2018, www.nobel.gen.tr merah-dan-manfaatnya-bagi-kesehatan/. [Accessed June 11th, 2016].
http://www.organikilo.co/2014/12/manfaat-airrebusan- kedelai-untuk.html. [accessed Mei 20th 2016].
Amin, M., et al., In vitro screening of lactic acid bacteria isolated from gastrointestinal tract of Atlantic Salmon (Salmo salar) as probiont candidates. Aquaculture International, 2016. 23: p. 1-14.
Amin, M., et al., Isolation of alginate lyaseproducing bacteria and screening for their potential characteristics as abalone probionts. Aquaculture Research, 2017. 48(11): p. 5614-5623.
Amin, M., Screening of Cellulose-Degrading Bacteria Associated with Gastrointestinal Tract of Hybrid Abalone as Probiotic Candidates. International Journal of Aquaculture, 2016. 6.
Ali, M., et al., Stirred bioreactor for the robustness production of recombinant gst. Vp28 in fed-batch cultivation of Escherichia coli. Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2015. 16(3): p. 245.
Rostinawati, T., Skrining dan identifikasi bakteri penghasil enzim kitinase dari air laut di perairan Pantai Pondok Bali. Abstrak, 2008.
Ratna, S., Mikrobiologi Dasar Dalam Praktek Teknik dan prosedur Dasar Laboratorium. Penerbit Gramedia, Jakarta, 1993.
https://www.nutritionix.com/food/palm-sugar, [accessed June 29th 2017].
Gandjar, I., Mikologi dasar dan terapan. 2006: Yayasan Obor Indonesia.
Irawan, M.A., Glukosa dan metabolisme energi. Sport Science Brief. Polton Sports Science and Performance Lab, 2007. 1(06).
https://www.sigmaaldrich.com/catalog/product, [accessed July 3rd 2017].
https://food.ndtv.com/food-drinks/palm-sugarand- its-health-benefits-is-it-really-good-for-you-1292110, [accessed August 23rd 2017].