Antimicrobial activities of basidiocarp, submerged mycelium and exopolysaccharide of some native basidiomycetes strains

Antimicrobial activities of basidiocarp, submerged mycelium and exopolysaccharide of some native basidiomycetes strains

In this study, extracts from basidiocarps, submerged grown mycelia and crude exopolysaccharide precipitates of Clavariadelphus truncatus (Quél.) Donk (Gomphaceae), Coprinus comatus (O.F. Müll.) Pers. (Agaricaceae), Ganoderma carnosum Pat. (Ganodermataceae), Cerrena unicolor (Bull.) Murrill, Laetiporus sulphureus (Bull.) Murrill, Lentinus strigosus (Schwein.) Fr., Lenzites betulina (L.) Fr. and Polyporus arcularius (Batsch) Fr. (Polyporaceae) were tested for antimicrobial activity. The activity was evaluated by hole-plate diffusion and disk diffusion tests using bacteria and yeasts. Vancomycine and fl uconazole were used as positive controls for bacteria and yeasts, respectively. The crude exopolysaccharides of Ganoderma carnosum, Polyporus arcularius, Lenzites betulina, Cerrena unicolor; and basidiocarps of Ganoderma carnosum, Laetiporus sulphureus, Coprinus comatus, Lenzites betulina and Clavariadelphus truncatus have relatively high antimicrobial activity. Mycelial extracts from Cerrena unicolor showed the highest antimicrobial activity against the test cultures.

___

  • 1. Anke T. 1989. Basidiomycetes: A source for new bioactive secondary metabolites. Progress in Industrial Microbiology. 27: 51 – 66.
  • 2. Stamets P. 2002. Novel antimicrobials from mushrooms. HerbalGram. 54: 29-33.
  • 3. Lindequist U, Niedermeyer THJ, Jülich WD. 2005. The pharmacological potential of mushrooms. eCAM. 2: 285–299.
  • 4. Rosa LE, Machado KMG, Jacob CC, Capelari M, Rosa CA, Zani CL. 2003. Screening of Brazilian Basidiomycetes for antimicrobial activity. Memórias do Instituto Oswaldo Cruz. 98: 967-974.
  • 5. Kavanagh F, Hervey A, Robbins WJ. 1950. Antibiotic substances from Basidiomycetes. VI. Agrocybe dura. Proceedings of the National Academy of Sciences USA. 36: 102-106.
  • 6. Musílek V, Černá J, Šašek M, Semerdžieva M, Vondráček M. 1969. Antifungal antibiotics of the Basidiomycete Oudemensiella mucida I. Isolation and cultivation of a producing strain. Folia Microbiologica. 14: 277-387.
  • 7. Semerdžieva M. 1983. Antifungal activity of submerged culture of Agrocybe aegerita. Sixteenth Annual Congress of the Czechoslovak Society for Microbiology. October 21-23. Banská Bystrica.
  • 8. Ishikawa NK, Fukushi Y, Yamaji K, Tahara S, Takahashi K. 2001. Antimicrobial cuparene-type sesquiterpenes, enokipodins C and D, from a mycelial culture of Flammulina velutipes. Journal of Natural Products. 64: 932-934.
  • 9. Omolo JO, Anke H, Sterner O. 2002. Hericenols A-D and a chromanone from submerged cultures of a Stereum species. Phytochemistry. 60: 431-435.
  • 10. Suay I, Arenal F, Asenio FJ, Basilio A, Cabello MA, Diez MT, Garcia JB, del Val AG, Gorrochategui J, Hernandez P, Pelaez F, Vicente MF. 2000. Screening of basidiomycetes for antimicrobial activities. Antonie van Leeuwenhoek. 78: 129-139.
  • 11. Yamaç M, Bilgili F. 2006. Antimicrobial activities of fruit bodies and / or mycelial cultures of some mushroom isolates. Pharmaceutical Biology. 44: 660-667.
  • 12. Zjawiony JK. 2004. Biologically active compounds from Aphyllophorales (polypore) fungi. Journal of Natural Products. 67: 300-310.
  • 13. Chihara G. 1992. Immunopharmacology of lentinan, a polysaccharide isolated from Lentinus edodes: Its application as a host defense potentiator. International Journal of Oriental Medicine. 17: 55-77.
  • 14. Sakagami H, Takeda M. 1993. Diverse biological activity of PSK (Krestin), a protein-bound polysaccharide from Coriolus versicolor (Fr.) Quél. In: Mushroom Biology and Mushroom Products (ed. Chang ST), pp. 237-245. Chinese University Press: Hong Kong.
  • 15. Wasser S.P, Weis A. 1999. Medicinal properties of substances occurring in higher basidimycetes mushrooms: current perspectives. International Journal of Medicinal Mushrooms. 1: 31-62.
  • 16. Ezeronye OU, Daba AS, Okwujiako IA, Onumajuru IC. 2005. Antibacterial effects of crude polysaccharide extracts from sclerotium and fruitbody (sporophore) of Pleurotus tuber-regium (Fried) Singer on some clinical isolates. International Journal of Molecular Medicine and Advance Sciences. 1: 202-205.
  • 17. Moser M. 1983. Keys to Agarics and Boleti, Gustav Fischer Verlag, Stuttgart.
  • 18. Breitenbach J, Kranzlin F. 1986. Fungi of Switzerland, Volume 2. Nongilled Fungi, Verlag mycologia, Luzern.
  • 19. Breitenbach J, Kranzlin F. 1991. Fungi of Switzerland, Volume 3. Boletes and Agarics 1. part, Verlag mycologia, Luzern.
  • 20. Breitenbach J, Kranzlin F. 1995. Fungi of Switzerland, Volume 4 .Agarics 2. part, Verlag mycologia, Luzern.
  • 21. Ellis MB, Ellis CP. 1990. Fungi without gills (Hymenomycetes and Gasteromycetes). Chapman and Hill, London.
  • 22. Kim SW, Hwang HJ, Park JP, Cho YJ, Song CH, Yun JW. 2002. Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media. Letters in Applied Microbiology. 34: 56- 61.
  • 23. Bauer AW, Kirby WMM, Sheriss JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 45: 493–496.
  • 24. Carron RA, Marran JM, Montero-Fernandozlago L, Dominguez AA. 1987. Antimicrobial properties of some extracts obtained from some mediterranean plants of medicinal value. Plantes Medicinales et Phytotherapie. 21: 195-202.
  • 25. Okamoto K, Shimada A, Shirai R, Sakamoto H, Yoshida S, Ojima F, Ishiguro Y, Sakai T, Kawagishi H. 1993. Antimicrobial chlorinated orcinol derivatives from mycelia of Hericium erinaceum. Phytochemistry. 34: 1445-1446.
  • 26. Kawagishi H, Mitsunaga SI, Yamawaki M, Ido M, Shimada A, Knoshita T, Murata T, Usui T, Kimura A, Chiba S. 1997. A lectin from mycelia of the fungus Ganoderma lucidum. Phytochemistry. 44: 7-10.
  • 27. Wichlacz M, Ayer WA, Trifonov LS, Chakravarty P, Khasa D. 1999. A caryophyllene-related sesquiterpene and two 6,7-Seco-caryophyllenes from liquid cultures of Hebeloma longicaudum. Journal of Natural Products. 62: 484-486.
  • 28. Hirasawa M, Shouji N, Neta T, Fukushima K, Takada K. 1999. Three kinds of antibacterial substances from Lentinus edodes (Berk.) Sing. (Shiitake, an edible mushroom). International Journal of Antimicrobial Agents. 11. 151-157.
  • 29. Hatvani N. 2001. Antibacterial effect of the culture fl uid of Lentinus edodes mycelium grown in submerged liquid culture. International Journal of Antimicrobial Agents. 17. 71-74.
Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi