Antimicrobial activity and Toxicity of Punica granatum L., peel
Antimicrobial activity and Toxicity of Punica granatum L., peel
Methanols extract of the Punica granatum L. (Punicaceae) peel was screened for antimicrobial activity against enteric pathogenic bacteria. Antimicrobial tests were carried out using the disk diffusion assay and broth dilution method. The extract showed favorable antimicrobial activity against enteric pathogenic bacteria tested with a minimum inhibitory concentration (MIC) value of 12.50 mg/ml. Furthermore, the extract was also tested against brine shrimp for toxicity. The extract exhibited no significant toxicity (LC50 = 1.42 mg/ml) against Anemia salina. The study confirms the possible antimicrobial potentiality of the peel extract of P. granatum. The study shows that the extract is a favorable antimicrobial agent with potential applications in public health against diseases.
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- [1]. Lansky EP, Newman RA. 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology. 109: 177-206
- [2] Jones ME, Draghi DC, Thornsberry C, Karlowsky JA, Sahm DF, Wenzel RP. 2004. Emerging resistance among bacterial pathogens in the intensive care unit - a European and North American Surveillance study (2000-2002). Annals of Clinical Microbiology and Antimicrobials. 3: 14.
- [3] Alzoreky NS, Nakahara K. 2003. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology. 80: 223-30.
- [4] Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by standardized single disc method. American Journal of Clinical Pathology. 36: 493-6.
- [5] Chattopadhyay D, Maiti K, Kundu AP, Chakrabarty MS, Bhadra R, Mandal SC, Mandal AB. 2001. Antimicrobial activity ofAlstonia macrophylla: folklore of Bay Islands. Journal of Ethnopharmacology. 77: 49-55.
- [6] Burrowes OJ Hadjicharalambous C Diamond G Lee TC. 2004. Evaluation of antimicrobial spectrum and cytotoxic activity of pleurocidin for food applications. Journal of Food Science. 69:66-71.
- [7] Meyer BN, Ferrigini RN, Jacobsen LB, Nicholas DE, McLaughlin JL. 1982. Brine shrimp: A convenient general bioassay for active plant constituent. Planta Medica. 45: 31-35.
- [8] Geran RI, Greenberg HM, McDonald M, Abbott BJ. 1972. Protocols for screening chemical agents and natural products against animal tumors and other biological s. Cancer Chemotherapy Reports. 33: 1-17.
- [9] Simionatto E, Porto C, da Silva UF, Squizani AMC, Dalcol II, Morel AF. 2005. Composition and antimicrobial activity of the essential oil from Aloysia sellowii. Journal of Brazilian Chemical Society. 16:1458-1462.
- [10] GisleneGFN, Juliana L, Paulo CF, Giul|ana LS. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology. 31: 247-256.
- [11] Kubo L, Muroi H, Himejima M.1993. Structure-antibacterial activity relationships of anacardic acids. Journal of Agriculture and Food Chemistry. 41: 1016-1019.