Defne Yaprağından (Laurus Nobilis L.) Fenolik Bileşiklerin Ekstraksiyonunda Farklı Yöntemlerin Karşılaştırılması
Bu çalışmada, mikrodalga destekli ve enzim destekli ekstraksiyon verimliliği çözgen ekstraksiyonu ile karşılaştırılmıştır. Defne yaprağı ekstraktında ekstraksiyon verimleri fenolik içerik ve antioksidan kapasitesi açısından değerlendirilmiştir. Geleneksel ekstraksiyon, enzim destekli ve mikrodalga destekli ekstraksiyon olarak üç farklı ekstraksiyon yönteminden elde edilen ekstraktların toplam fenolik içeriği (mg GAE/g) sırasıyla 23.29±0.02, 32.45±0.02 ve 30.49±0.02 olarak bulunmuştur. Toplam fenolik içerik için en yüksek değer enzim destekli ekstraksiyonda belirlenmiştir. Üç farklı ekstraksiyon yönteminden elde edilen ekstraktların DPPH radikal süpürme aktivitesi (%) sırasıyla %36.91±0.05, %50.72±0.27 ve %41.51±0.09 olarak bulunmuştur. Toplam fenolik içerik gibi, DPPH radikal süpürme aktivitesi için en yüksek değer enzim destekli ekstraksiyonda belirlenmiştir. Ayrıca defne yaprağının toplam kuru madde, toplam kül, toplam protein, askorbik asit ve toplam klorofil içeriği belirlenmiştir.
Anahtar Kelimeler:
enzim destekli ekstraksiyon, mikrodalga destekli ekstraksiyon, defne yaprağı, fenolik bileşikler
Comparison of Different Methods in the Extraction of Phenolic Compounds from Bay Leaf (Laurus nobilis L.)
In this study, the microwave-assisted and enzyme-assisted extraction efficiency were compared to solvent extraction. The extraction efficiencies were evaluated in bay leaf extract in terms of phenolic content and antioxidant capacity. The total phenolic content (mg GAE/g) of the extracts from three different extraction methods as a solvent, enzyme-assisted, and microwave-assisted extraction was found 23.29±0.02, 32.45±0.02, and 30.49±0.02, respectively. The highest value for the total phenolic content was found from the enzyme-assisted extraction. DPPH radical scavenging capacity (%) of the extracts from three different extraction methods was found at 36.91%±0.05, 50.72%±0.27, and 41.51%±0.09, respectively. Like the total phenolic content, the highest value for the DPPH radical scavenging capacity was found from the enzyme-assisted extraction. In addition, total dry matter, total ash, total protein, ascorbic acid, and total chlorophyll content of the bay leaf were analyzed.
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- Yayın Aralığı: Yılda 2 Sayı
- Başlangıç: 2018
- Yayıncı: Oktay YILDIZ