Okan Kadir NOHUT, Cem Tolga GÜRKANLI, İbrahim ÖZKOÇ

Türkiye’nin Samsun İlindeki Bazı Yabani Baklagil Türlerini Nodüle Eden Mesorhizobium Türlerinin Çeşitliliği

Bu çalışmada Türkiye’nin Samsun ilinden toplanan iki yabani Baklagil türünün (Argyrolobium biebersteinii (Ball in Feddes) and Lotus angustissimus L.,) mikrosimbiyontları geleneksel ve moleküler yöntemler kullanılarak incelenmiştir. Toplamda orijinal konakçıları üzerinde aktif kök nodülleri oluşturan beş adet rizobiyal izolat incelenmiştir. Bu izolatların teşhisleri 16S rDNA ve recA olmak üzere iki genin filogenetik analizleri ile yapılmıştır. Sonuç olarak OKN-1 ve OKN-4 Mesorhizobium tarimense ve OKN-3 M. japonicum olarak teşhis edilmişlerdir. Diğer taraftan diğer iki izolatımız OKN-7 ve OKN-10 potansiyel iki yeni Mesorhizobium türü olarak ortaya çıkmıştır. Sonuç olarak bu çalışmada Türkiye’den Mesorhizobium cinsine ait ilk moleküler olarak tanımlanmış örnekleri sunmaktayız. Ayrıca M. tarimense ve M. japonicum türleri için Türkiye’den ve Avrupa’dan ilk kayıtları da vermekteyiz. Ek olarak M. tarimense ve M. japonicum izolatlarımız, bu iki türün orijinal konaklarından ve lokalitelerinden tanımlanmasından sonra bildirilen ilk izolatlardır. Bu çalışmada potansiyel olarak yeni iki Mesorhizobium türü için de moleküler kanıtlar sunuyoruz.

Anahtar Kelimeler:

Filogeni, Mesorhizobium, Nodülasyon, Samsun

Diversity of Mesorhizobium Species Nodulating Some Wild Legumes in Samsun Province of Turkey

In this study microsymbionts of two wild legumes, Argyrolobium biebersteinii (Ball in Feddes) and Lotus angustissimus L., collected from Samsun province of Turkey were investigated using conventional and molecular methods. A total of five rhizobial isolates which produced active root nodules on their original hosts were considered. Identifications of the isolates were depending on the phylogenetic analyses of two housekeeping genes, 16S rDNA and recA. As a result, isolates OKN-1 and OKN-4 identified as Mesorhizobium tarimense and OKN-3 identified as M. japonicum. On the other hand, our other two isolates OKN-7 and OKN-10 appeared as two potentially new Mesorhizobium species. In this study we present the first Mesorhizobium isolates identified using valid molecular methods from Turkey. We also give the first reports of M. tarimense and M. japonicum from Turkey and Europe. Additionally, our M. tarimense and M. japonicum isolates are the first isolates reported after the description of these two species from their original hosts and locations. In this study we also present molecular evidences for two potentially new Mesorhizobium species.

Keywords:

Mesorhizobium, Nodulation, Phylogeny, Samsun,

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