THE EFFECT OF MORNING AND AFTERNOON EXERCISE ON GHRELIN AND VASPIN LEVELS

Introduction: Ghrelin and Vaspin have important roles in energy metabolism, body composition and cardiovascular health, and are closely associated to exercise. The aim of the study was to determine the levels of Ghrelin and Vaspin in saliva and urine samples related to morning and afternoon exercise. Material and Method: The anthropometric measurements ofyoung healthy football players (n:11) were calculated. The athletes performed 60 minutes of mild exercise at 60% maximum heart rate(HRmax). Saliva and urine samples were taken before and after exercise (at 10.00 and 16.00 for pre-exercise, at 11.00-17.00 for post exercise). The Ghrelinand Vaspinlevels were measured using the ELISA method. Statistical analysis was performed with repeated measurements ANOVA test and Pearson Correlation analysis. Results: SalivaGhrelin and Vaspinlevels indicated a positive correlation (at 10:00 and 16:00) before exercise (r = 0.664, p = 0.026; r = 0.657, p = 0.028 respectively) and samples from 17:00 demonstrated a significant increasecompared to 10.00(p=0.017, p=0.007). A negative correlation was observed between Ghrelinlevels and body mass index (BMI) (r = – 0.667, p = 0.025). A difference in protein measurements at immediately before and immediately after exercise (between 10.00 and 11.00 and between 16.00 and 17.00) was not determined. Conclusion: Consequently, the expression of these proteins did not change with low intensity exercise. Compared with in the morning, we detected increased levels of both these proteins in the saliva samples in the afternoon. Since these proteins are at a higher level later in the day, they can contribute positively to exercise performance in the afternoon.

SABAH VE ÖĞLEDEN SONRA YAPILAN EGZERSİZİN GHRELİN VE VASPİN DÜZEYLERİNE ETKİSİ

Giriş: Ghrelin ve Vaspin enerji metabolizmasında, vücut kompozisyonunda ve kardiyovasküler sağlıkta önemli rol oynamaktadır ve egzersizle yakından ilişkilidir. Çalışmanın amacı, sabah ve öğleden sonra yapılan egzersizde Ghrelin ve Vaspin seviyelerindeki değişimleri idrar ve tükürükte belirlemektir. Gereç ve Yöntem:Genç sağlıklı futbolcuların antropometrik ölçümleri (n: 11) hesaplandı. Sporcular % 60 maksimum kalp atım hızında (HRmax) 60 dakikalık hafif egzersiz yaptılar. Egzersiz öncesi ve sonrası tükürük ve idrar örnekleri alındı (egzersiz öncesi saat 10.00 ve 16.00, egzersiz sonrası saat 11.00-17.00). Ghrelin ve Vaspin düzeyleri, ELISA yöntemi kullanılarak ölçüldü. İstatistiksel analiz, tekrarlanan ölçümlerde ANOVA testi ve Pearson Korelasyon analizi kullanılarak yapıldı. Bulgular: Tükürük Ghrelin ve Vaspin düzeyleri, egzersiz öncesi (saat 10:00 ve 16:00’da) pozitif bir korelasyon gösterdi (sırasıyla r = 0.664, p = 0.026; r = 0.657, p = 0.028) ve saat 17:00'da alınan örnekler 10.00 ile karşılaştırıldığında anlamlı olarak artış gösterdi (p = 0.017, p = 0.007). Ghrelin düzeyleri ile vücut kütle indeksi (BMI) arasında negatif bir korelasyon gözlendi (r = - 0.667, p = 0.025). Egzersizden hemen önce ve hemen sonra alınan örnekler arasında (10.00 ile 11.00 arasında ve 16.00 ile 17.00 arasında) protein ölçümleri açısından bir fark belirlenmedi. Sonuç: Sonuç olarak, bu proteinlerin ekspresyonu düşük yoğunluklu egzersiz etkisi ile değişmedi. Her iki proteinin öğleden sonra alınan tükürük numunelerinde öğleden önceye göre artmış olduğunu tespit ettik. Bu proteinler günün ilerleyen saatlerinde daha yüksek düzeyde olduklarından öğleden sonra yapılan egzersiz performansına olumlu katkıda bulunabilirler.

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