80% inhibition in ELISA, which were considered as a high titre colostrum associated with E.coli K99, 28 samples had no any anti E.coli Ab, 71 samples showed degree of positivity between 1+ and 2+ and 14 samples were 3+. The present study showed that only 5.83% of colostrum samples had high titre of anti E.coli K99 Ab for passive transfer to protect the calves against diarrhea due to E.coli K99. Therefore, it is recommended that vaccination of pregnant cows is necessary for enhancing the titre of Ab in a high percent of them". "> [PDF] Evaluation of antibody level against E. coli K99 in colostrum of cows in suburbs of Tehran, Iran | [PDF] Evaluation of antibody level against E. coli K99 in colostrum of cows in suburbs of Tehran, Iran 80% inhibition in ELISA, which were considered as a high titre colostrum associated with E.coli K99, 28 samples had no any anti E.coli Ab, 71 samples showed degree of positivity between 1+ and 2+ and 14 samples were 3+. The present study showed that only 5.83% of colostrum samples had high titre of anti E.coli K99 Ab for passive transfer to protect the calves against diarrhea due to E.coli K99. Therefore, it is recommended that vaccination of pregnant cows is necessary for enhancing the titre of Ab in a high percent of them". ">

Evaluation of antibody level against E. coli K99 in colostrum of cows in suburbs of Tehran, Iran

Evaluation of antibody level against E. coli K99 in colostrum of cows in suburbs of Tehran, Iran

"One hundred and twenty colostrum samples were obtained from 120 randomly selected populations of Holstein dairy cows just on day of calving in the south of Tehran. The competitive ELISA kit for serodiagnosis of anti Escherichia coli K99 (F5) attachment factor in colostrum (Biox diagnostics) were used. Out of 120 samples, 7 cases (5.83%) showed >80% inhibition in ELISA, which were considered as a high titre colostrum associated with E.coli K99, 28 samples had no any anti E.coli Ab, 71 samples showed degree of positivity between 1+ and 2+ and 14 samples were 3+. The present study showed that only 5.83% of colostrum samples had high titre of anti E.coli K99 Ab for passive transfer to protect the calves against diarrhea due to E.coli K99. Therefore, it is recommended that vaccination of pregnant cows is necessary for enhancing the titre of Ab in a high percent of them".

___

  • 1- Banks KL.: Host defense in the newborn animal, 1982; 15; 181(10):1053-6.
  • 2- Caspari G. R.: The influence of colostral leukocytes on the course of an experimental Esherichia coli infection and serum antibodies in neonatal calves, Veterinary Immunology and Immunopathology, 1993; 35, 3-4, 275-288.
  • 3- Contrepois M., Dubourguier H. C., Parodi A. L., Girardeau J. P., Oillier J. L., : Septicemic Escherichia coli and experimental infection of calves, Veterinary Microbiology, 1986; 12, 2, 109-118.
  • 4- Harley W. M., Bunn T. O.: Vaccines for preventing enterotoxigenic Escherichia coli infections in farm animals, Vaccine; 1993, 11, 2, 213-220.
  • 5- Lissner R., Schmidt H., Karch H.: A standard immunoglobulin preparation produced from bovine colostra shows antibody reactivity and neutralization activity against Shiga-like toxins and EHEC-hemolytic of Escherichia coli O157:H7. Infection, 1996; 24(5): 378-383.
  • 6- Pirro F., Wieler L.H., Railing K., Bauerfeind R., Baljer G.: Neutralizing antibodies against Shiga- like toxins from Escherichia coli in colostra and sera of cattle, Veterinary Microbiology, 1995; 43(2-3),131-141.
  • 7- Silverstein A.M.: Paul Ehrlich: The founding of pediatric Immunology, Cellular Immunology, 1996; 174:1-6.
  • 8- Tizzard I.: An introduction to veterinary immunology.7th edition, Philadelphia, WB Saunders Co., 2004; p: 49-50.
  • 9- Valente C., Fruagnati G., Tesei B., Ciorba A., Cardaras P., Floris A., Bordoni E.: Vaccination of pregnant cows with K99 antigen of enterotoxigenic Escherichia coli protection by colostrum in newborn calves, Comparative immunology, Microbiology and Infectious Disease, 1988; 11, 3-4, 189-198.
  • 10- Xu L. B., Chen L., Gao W., He Du K., : Bovine immune colostrum against 17 strains of diarrhea bacteria and in vitro and in vivo effects of its specific IgG, Vaccine, 2006; 24, 2131- 2140.
  • 11- Xu RJ.: Development of the newborn GI tract and its relation to colostrum/milk intake: a review, Reproduction, Fertility and development, 1996; 8: 35-48.