Effect of Different Level of Psyllium Supplementation to Horse Diet on in vitro Fermentation Parameters and Methane Emission

Bu çalışmanın amacı, at rasyonlarına psilyum ilavesinin metan salınımı ve sindirim parametrelerine etkisinin at dışkısı inokulumu kullanarak in vitro sindirim tekniği ile saptanmasıdır. At rasyonlarına 0 (kontrol grubu), 5, 10, 20 ve 40 g/kg kuru madde (KM) (deneme grupları) düzeyinde psilyum (Psyllium Husk, Solgar) ilavesinin in vitro toplam gaz ve metan üretimi ile metabolik enerji (ME) organik madde sindirimi (OMS), amonyak azotu (NH3-N), kısa zincirli yağ asitleri (SCFA) ve pH değerlerine etkisi belirlenmiştir. İn vitro sindirim denemesi 100 ml'lik cam şırıngalar (Model Fortuna, Almanya) içinde 24 saat süresince 39.0±0.2°C'lik inkübasyonda gerçekleştirilmiştir. Çalışmada, in vitro toplam gaz üretimi kontrol grubuna (181 ml/g KM) göre deneme gruplarında (130 ml/g KM'e kadar) linear olarak azalmıştır (P

At Rasyonuna Farklı Düzeylerde İlave Edilen Psilyum'un in vitro Fermentasyon Parametreleri ve Metan Salınımına Etkisi

The purpose of this study was to determine the effect of psyllium addition to horse diets on methane emissions and digestion parameters by in vitro digestion technique using horse feces as inoculum. The effect of 0 (control group), 5, 10, 20 and 40 g/kg DM (Dry matter) (treatment groups) psyllium (Psyllium Husk, Solgar, UK) supplementations to horse diet were determined on in vitro total gas and methane production, metabolisable energy (ME), organic matter digestion (OMD), ammonia nitrogen (NH3-N), short chain fatty acids (SCFA) and pH value. In vitro digestibility technique was performed with using glass syringes of 100 ml volumes (Model Fortuna, Germany) at 39.0±0.2°C for 24 hour incubation. In the study, in vitro total gas production was linearly decreased in treatment groups (up to 130 ml/g DM) compared to control group (181 ml/g DM) (P

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  • Almeida, F.A., Martins, J.A., Silva, V.P., Trigo, P., Pereira, M.B., Galvao, P.M., Silva, A.T., Miranda, A.C.T., 2012. In vitro Gas Production from Colon Contents in Electrolyte Supplemented Horses. European Federation of Animal Science. Wageningen Academic Publishers. The Netherlands.
  • A.O.A.C., 1995. Association of Official Analytical Chemists. Official Methods of Analysis (15th ed.,). Arlington, VA.
  • Biehler, E., Mayer, F., Hoffman, L., Krause, E., Bohn, T., 2010.
  • Comparison of 3 spectrophotometric methods for carotenoid determination in frequently consumed fruits and vegetables. Journal of Food Science 75, 55- 61.
  • Calabro, S., Carciofi, A.C., Musco, N., Tudisco, R., Gomes, M.O.S., Cutrignelli, M.I., 2013. Fermentation characteristics of several carbohydrate sources for dog diets using the in vitro gas production technique. Italian Journal of Animal Science 12, 21-27.
  • Cannon, S.J., 2009. Effects of Psyllium in Milk Replacers for Neonatal Dairy Calves. Master Thesis. University of Illinois at Urbana-Champaign. Crutzen, P.J., Aselmann, I., Seiler, W., 1986. Methane production by domestic animals, wild ruminants, other herbivorous fauna, and humans. Tellus 38B, 271-284.
  • Deaker, J.M., Young, M.J., Fraser, T.J., Rowarth, J.S., 1994.
  • Carcass, liver and kidney characteristics of lambs grazing plantain (Plantago lanceolata), chicory (Cichorium intybus), white clover (Trifolium repens) or perennial ryegrass (Lolium perenne). Proceedings of the New Zealand Society of Animal Production 54, 197- 200.
  • Ellis, J.L., Kebreab, E., Odongo, N.E., McBride, B.W., Okine, E.K., France, J., 2007. Prediction of methane production from dairy and beef cattle. Journal of Dairy Science 90, 3456-3467.
  • Fischer, M.H., Yu, N., Gray, G.R., Ralph, J., Anderson, L., Marlett, J.A., 2004. The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk). Carbohydrate Research 339, 2009-2017.
  • Fons, F., Rapior, S., Gargadennec, A., Andary, C., Bessiere, J.M., 1998. Volatile components of Plantago lanceolata (Piantaginaceae). Acta Botanica Gallica 145, 265-269.
  • Frape, D., 2004. Equine Nutrition and Feeding. Third Edition. Blackwell Publishing, pp. 1-636.
  • Getachew, G., Makkar, H.P.S., Becker, K., 2002. Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal of Agriculture Science 139, 341-352.
  • Goel, G., Makkar, H.P.S., Becker, K., 2008. Effects of Sesbania sesban and Carduus pycnocephalus leaves and fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage- and concentrate-based feeds to methane.
  • Animal Feed Science and Technology 147, 72-89.
  • Hammock, P.D., Freeman, D.E., Baker, G.J., 1998. Failure of psyllium mucilloid to hasten evaluation of sand from the equine large intestine. Veterinary Surgergy 27, 547-554.
  • Hook, S.E., Wright, A.D.G., McBride, B.W., 2010.
  • Methanogens: Methane producers of the rumen and mitigation strategies. Archaea 2010, 1-11.
  • Hotwagner, K., Iben, C., 2008. Evaluation of sand from the equine intestine with mineral oil, with and without psyllium. Journal of Animal Physiology and Animal Nutrition 92, 86-91.
  • IPCC, 2014. Summary for Policymakers. In: Climate Change 2014: Mitigation of Climate Change. Contribution of Working Group III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change [Edenhofer, O., R. Pichs-Madruga, Y. Sokona, E.
  • Farahani, S. Kadner, K. Seyboth, A. Adler, I. Baum, S.
  • Brunner, P. Eickemeier, B. Kriemann, J. Savolainen, S.
  • Schlömer, C. von Stechow, T. Zwickel and J.C. Minx (eds.)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA.
  • Kamalak, A., Canbolat, O., Sahin, M., Gurbuz, Y., Ozkan, C.O., 2005. The effect of polyethylene glycol (PEG 8000) supplementation on in vitro gas production kinetics of leaves from tannin containing trees. South African Journal of Animal Science 35, 229-237.
  • Kara, K., 2015. In vitro methane production and quality of corn silage treated with maleic acid. Italian Journal Animal Science 14, 718-722.
  • Kara, K., Aktug, E., Cagri, A., Guclu, B.K., Baytok, E., 2015a.
  • Effect of formic acid on in vitro ruminal fermentation and methane emission. Turkish Journal AgricultureFood Science Technology 3, 856-860.
  • Kara, K., Guclu, B.K., Aktug, E., Baytok, E., 2015b. The determination of nutrient matter composition and in vitro digestion parameters of narrow-leaf plantain (Plantago lanceolata) in ruminant. Journal of Health Sciences. 24, 149-155.
  • Kara, K., Guclu, B.K., Baytok, E., 2015c. Comparison of nutrient composition and anti-methanogenic properties of different Rosaceae species. Journal of Animal and Feed Science 24, 308-314.
  • Kholif, A.E., Baza-Garcia, L.A., Elghandour, M.Y., Salem, A.Z.M., Barbabosa, A., Dominguez-Vara, I.A., 2015. In vitro assement of fecal inocula from horses fed on high-fiber diets with fibrolytic enzymes addition on gas, methane and carbon dioxide productions as indicators of hindgut activity. Journal of Equine Veterinary Science 39, 44-50
  • Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D., Schneider, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agriculture Science 93, 217-222.
  • NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th Revised Edition, Subcommittee on Dairy Cattle Nutrition, Committee on Animal Nutrition, Board on Agriculture and Natural Resources, National Research Council, National Academy Press, Washington, D.C.
  • Rafiu, O.Y., Noor, Z.Z., Abba, A.H., Hassan, M.A.A., Din, M.F.M., 2012. Greenhouse Gas Emissions: Quantifying Methane Emissions from Livestock. American Journal of Engineering and Applied Sciences 5, 1-8.
  • Samuelsen, A.B., 2000. The traditional uses, chemical constituents and biological activities of Plantago major L. A review. Journal of Ethnopharmacology 71, 1-21.
  • Santos, A.S., Cabo, A.D., Lima, S.M., Ferreira, L.M., Rodrigues, M.A.M., 2012. Fermentation parameters and total gas production of equine caecal and faecal inocula. Forages and grazing in horse nutrition. European Federation of Animal Science. Wageningen Academic Publishers. The Netherlands, pp. 55-58
  • Stewart, A.V., 1996. Plantain (Plantago lanceolata)-a potential pasture species. Proceedings of the New Zealand Glassland Association 58, 77-86.
  • Sunvold, G.D., Fahey, G.C., Merchen, N.R., Reinhart, G.A., 1995. In vitro fermentation of selected fibrous substrates by dog and cat fecal inoculum: influence of diet composition on substrate organic matter disappearance and short-chain fatty acid production. Journal of Animal Science 73, 1110-1122.
  • Swanson, K.S., Grieshop, C.M., Clapper, G.M., Shields, R.G., Belay, T., Merchen, N.R., Fahey, G.C., 2001. Fruit and vegetable fiber fermentation by gut microflora from canines. Journal of Animal Science 79, 919-926.
  • Sweney, C.R., 2012. In vivo and in vitro digestbility of a complete pelleted feed in horses. Master Thesis. The Faculty of California Polytechnic State, CA, USA. pp. 1- 117.
  • Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B., France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology. 48, 185-197.
  • Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583-3597.
  • Washington, N., Harris, M., Mussellwhite, A., Spiller, R.C., 1998. Moderation of lactulose-induced diarrhoea by psyllium: effects on motility and fermentation. American Journal of Clinical Nutrition 67, 317-321.
İstanbul Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 0250-2836
  • Yayın Aralığı: Yılda 2 Sayı
  • Yayıncı: İstanbul Üniversitesi Yayınevi
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