Rumen BINEV, Yordan NIKOLOV, Lazarin LAZAROV, Tsanko HRISTOV, Krasimira UZUNOVA, Nely GROSEVA, Dian KANAKOV, Ivan VALCHEV

Aflatoksikozis ile deneysel olarak uyarılmış mulardlarda karaciğer fonksiyonları üzerine incelemeler

Kanatlılarda aflatoksin В1 (AFB1)’in toksik etkilerine en hassas olan ördek yavrularıdır. Bu çalışmada mulard ördeklerde AFB1’in karaciğer morfolojisi, plazma aspartat amino-transferaz (AST), alanin amino-transferaz (ALT), alkalin phosphataz (ALP), laktat dehidrogenaz (LDH), γ-glutamiltransferaz (γ-GT), albümin, kan glukoz, ve plazma total protein (TP) üzerine toksik etkileri incelenmiştir. Deney 20 adet 10 günlük yaştaki ördek yavrularından 4 grup ile gerçekleştirilmiştir. Her grupta 10 ördekten oluşan 3 alt grup bulunmaktadır. Grupların oluşturulmasında; grup І – kontrol (türe ve yaşa göre standart olan başlangıç yemi verilmiştir), grup ІІ – deneysel, 0,5 mg/kg AFB1 içeren yem verilmiştir, grup ІІI–0,8 mg/kg AFB1 içeren yem verilmiştir ve grup IV – yeme 0,5 mg/kg AFB1 ve 2 g/kg Mycotox NG katılmıştır. Deney 42 gün sürmüştür. Grup ІІ ve ІІI’te makroskobik olarak karaciğerin büyümüş, yuvarlaklaşmış, sarı renkte ve gevrek yapıda olduğu gözlenmiştir. Histopatolojik olarak toksin alınma oranına göre değişen derecelerde distrofi tespit edilmiştir. Yeme mikosorbent Mycotox NG katılması kan biyokimyasal parametrelerde sapmaları düzeltmiş ve histolojik lezyonların şiddetini ve yaygınlığını azaltmıştır

Investigations on liver function in mulards with experimentally induced aflatoxicosis

Ducklings are among the most sensitive avian species to the toxic effects of aflatoxin В1 (AFB1). In thisexperiment, the toxic effects of AFB1 on liver morphology, blood plasma aspartate amino-transferase (AST), alanineamino-transferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), γ-glutamyltransferase (γ-GT),albumin, blood glucose, and plasma total protein (TP) were established in mulard ducks. The experiment was carriedout with four groups of 20 10-day-old ducklings each. Each group included three subgroups with 10 birds. The groupswere as followed: group І – control (which received standard feed according to the species and age), group ІІ –experimental, which received compound feed with 0.5 mg/kg AFB1, group ІІI– receiving compound feedsupplemented with 0.8 mg/kg AFB1 and group IV – compound feed supplemented with 0.5 mg/kg AFB1 and 2g/kgMycotox NG. The experiment lasted for 42 days. Macroscopically, livers were enlarged, rounded, with yellowishcolour and a frail consistency in group ІІ and ІІI. Histopathologically, a various extent of dystrophy was detecteddepending on the dose of ingested toxin. The supplementation of compound feed with the mycosorbent Mycotox NGimproved deviations in blood biochemical parameters and substantially reduced the severity and prevalence ofhistological lesions.

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