Antimicrobial sensitivity of campylobacter jejuni poultry isolates from the republic of Bulgaria

2009 yılının ilk yarısında Bulgaristan'da 6 farklı küçük tavuk çiftliğinde bulunan etlik piliçlerden toplam 110 sekum örneği toplandı ve 45 adet Campylobacter jejuni suşu izole edildi. C. jejuni izolatlarının yirmi dördünün (%53,3) çeşitli antibiyotiklere karşı dirençli olduğu belirlendi. Yüksek oranlarda tetrasiklin (%22,2), enrofloksasin(%13,4) ve ampisilin (%11,1)’e karşı dirençlilik saptandı. İki suş (%4,4) eritromisine dirençli bulundu. Minimum inhibisyon konsantrasyonuna bağlı olarak, izole edilen suşların tetrasikline karşı en yüksek değerinin 64 μg/ml olduğu belirlendi. Ampisiline dirençli dört izolatın MİK değeri 32 μg/ml ve birinin MİK değeri 16 μg/ml olarak saptandı.

Bulgaristan’da kanatlı kökenli campylobacter jejuni izolatlarının antibiyotik duyarlılıkları

A total of 110 caecal samples were collected from broiler chickens originating from 6 small poultry farms in Bulgaria during the first half of 2009, and (45) Campylobacter jejuni strains were isolated. Twenty four (53.3%) of the C. jejuni isolates were determined as resistant to several antibiotics. The highest percentage of resistance was observed against (tetracycline) - 22.2%, (enrofloxacin) - 13.4% and (ampicillin) - 11.1%. Two strains (4.4%) were resistant to erythromycin. The distribution of isolates on the basis of Minimal Inhibition Concentration revealed the highest value of 64 μg/ml to tetracycline in one of the strains. Four isolates resistant against ampicillin were with MIC of 32 μg/ml, and another one – with MIC 16 μg/ml.

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  • Allos, B.M., 2001. Campylobacter jejuni infections: update on emerging issues and trends. Clinical Infectious Diseases 32, 1201-1206.
  • Anonymous, 2007. Raport of the Task Force on Zoonoses Data Collection including a proposal for a harmonized monitoring scheme of antimicrobial resistance in Salmonella in fowl, turkeys and pigs and Campylobacter jejuni and Campylobacter coli in broilers. EFSA Journal 96, 1-46.
  • Avrien, L., Humbert, F.L., Hospitalier, R., 2003. Antimicrobial resistance in Campylobacter from broilers: association with production type and antimicrobial use. Veterinary Microbiology 96, 267-276.
  • Bardon, J., Kolar, M., Cekanova, L., Hejnar, P., Koukalova, D., 2009. Prevalence of Campylobacter jejuni and its resistance to antibiotics in poultry in the Czech Republic. Zoonoses Public Health April 56 (3), 111-116.
  • Chen, X., Naren, G.W., Wu, C.M., Wang, Y., Dai, L., Xia, L.N., Luo, P.J., Zhang, Q., Shen, J.Z., 2010. Prevalence and antimicrobial resistance of Campylobacter isolates in broilers from China. Veterinary Microbiology 144 (1-2), 133-139.
  • CLSI, 2006. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational supplement (M100-S16) and Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Tentative guidline M31- A3-Third Edition.
  • Endtz, H.P., Ruijs, G.J., Van Klingeren, B., Jansen, W.H., Van Der Reyden, T., Mouton, R.P., 1991. Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine. Journal of Antimicrobial Chemotherapy 27, 199-208.
  • Engberg, J., Aestrup, F.M., Taylor, D.E., Gerner- Smidt, P., Nachamkin, I., 2001. Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerging Infectious Diseases 7, 24-34.
  • Gallay, A., Prouzet-Maulèon, V., Kempf, I., Lehours, P., Labadi, L., Camon, C., Denis, M., De Valk, H., Desenclos, J.C., Megraud, F., 2007. Campylobacter antimicrobial drug resistance among humans, broiler chickens, and pigs. Emerging Infectious Diseases 13 (2), 259- 266.
  • Gibreel, A., Taylor, D.E., 2006. Macrolide resistance in Campylobacter jejuni and Campylobacter coli. Journal of Antimicrobial Chemotherapy 58, 243-255.
  • Hoge, C.W., Gambel, J.M., 1998. Trends in antibiotic resistance among diarrheal pathogens isolated in Thailand over 15 years. Clinical Infectious Diseases 26, 341-345.
  • Jacobs-Reitsma, W.F., Kan, C.A., Bolder, N.M., 1994. The induction of quinolone resistance in Campylobacter in broilers by quinolone treatment. Letters Applied Microbiology 19, 228- 231.
  • Leonard, E.E., Tompkins, L.S., Falkow, S., Nachamkin, I., 2004. Comparison of Campylobacter jejuni isolates implicated in Guillain-Barrė syndrome and strains that cause enteritis by a DNA microarray. Infection and Immunity 72, 1199-1203.
  • Mc Dermot, P.F., Bodies, S.M., English, L.L., White, D.G., Walker, R.D., Zhao, S., Simjee, S., Wagner, D.D., 2002. Ciprofloxacin resistance in Campylobacter jejuni evolves rapidly in chickens treated with fluoro- quinolons. Journal of Infectious Diseases 185, 837-840.
  • Norström, M., Johnsen, G., Hofshagen, M., Tharaldsen, H., Kruse, H., 2007. Antimicrobial resistance in Campylobacter jejuni from broilers and broiler house environments in Norvay. Journal of Food Protocols March 70 (3), 736-738.
  • OIE Terrestrial Manual, 2008. Campylobacter jejuni and Campylobacter coli. In Chapter 2.9.3, 1185-1191.
  • Saenz, Y., Zarazaga, M., Lantero, M., Gastanares, M., Baquero, F., Torres, C., 2000. Antibiotic resistance in Campylobacter strains isolated from animals, foods, and humans in Spain in 1997- 1998. Antimicrobial Agents and Chemotherapy 44, 267-271.
  • Skirrow, M.B., 1991. Epidemiology of Campylobacter enteritis. International Journal of Food Microbiology 12, 9-16
  • Skjøt–Rasmussen, L., Ethelberg, S., Emborg, H.D., Agresø, Y., Larsen, L.S., Nordentoft, S., Olsen, S.S., Ejlertsen, T., Holt, H., Nielsen, E.M., Hammerum, A.M., 2009. Trends in occurrence of antimicrobial resistance in Campylobacter jejuni isolates from broiler chicken meat, and human domestically acquired cases and travel associated cases in Denmark. International Journal of Food Microbiology 131 (2-3), 277-279.
  • Tetsuo, A., Kazuki, H., Kanako, I., Akemi, K., Toshiya, S., Yutaka, T., Toshio, T., 2007. Association of antimicrobial resistance in Campylobacter isolated from food-producing animals with antimicrobial use in farms. Journal of Infectious Diseases 60, 290-294.
  • Van Den Bogaard, A.E., Stobberingh, E.E., 1999. Antibiotic usage in animals: impact on bacteria resistance and public health. Drugs 58, 589-607.
  • Van Looveren, M., Daube, G., De Zutter, L., Dumont, J.M., Lammens, C., Wijdooghe, M., Vandamme, P., Jouret, M., Cornelis, M., Goossens, H., 2001. Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium. Journal of Antimicrobial Chemotherapy 48, 235-240.
  • Winn, C.W., Allen, S.D., Janda, M.W., Koneman, W.E., Procop, W.C., Schreckenberger, C.P., Woods, L.G., 2006. Curved Gram- negative Bacilli and Oxidase- Positive Fermenters: Campylobacteraceae and Vibrionaceae In: Darcy, P. (Sixth Edition) Koneman,s Color Atlas and Textbook of Diagnostic Microbiology. Lippincott Williams & Wilkins, Philadelphia, pp. 393-408.
  • Yuki, N., 2001. Infectious origins of, and molecular mimicry in, Guillain-Barrė and Fisher syndromes. Lancet Infectious. Diseases 1, 29- 37.
İstanbul Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 0250-2836
  • Yayın Aralığı: Yılda 2 Sayı
  • Yayıncı: İstanbul Üniversitesi Yayınevi
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