Yeşim ÖZTAYLAN, İsmet Burcu TÜRKYILMAZ, Refiye YANARDAĞ

VALPROİK ASİT UYGULANAN SIÇANLARDA ALFA LİPOİK ASİDİN LENS HASARI ÜZERİNE ETKİLERİ

Amaç: Valproik asit (VPA) kısa zincirli bir yağ asididir ve migren ile şizofreni tedavisinde kullanılır. Tedavi edici etkilerinin olmasına rağmen, bu ilacın yan etkileri serbest radikaller ile ilişkilidir ve bu yol ile birçok organ ve dokuyu etkilemektedir. Alfa lipoik asit (ALA), güçlü bir antioksidandır. Gereç ve Yöntem: Bu çalışmada, ALA’in VPA ile oluşturulan lens hasarı üzerine koruyucu etkileri araştırıldı. Dişi sıçanlar dört gruba ayrıldı: 1. grup, kontrol hayvanları (15 gün boyunca her gün mısır özü yağı verildi); 2. grup, ALA verilen grup (15 gün boyunca her gün 50 mg/kg verildi); 3. grup, VPA uygulanan grup (15 gün boyunca her gün 500 mg/kg uygulandı), 4. grup; Her gün aynı saatte ve dozda VPA ile ALA uygulanan grup, 16’ıncı günde, lens dokuları alındı. Bulgular: VPA uygulanmasından sonra, lens glutatyon düzeyleri ve glutatyon-S-transferaz aktiviteleri azalırken lipid peroksidasyonu ve protein karbonil seviyeleri, süperoksit dismutaz, glutatyon peroksidaz ve redüktaz, aldoz redüktaz ve sorbitol dehidrojenaz aktiviteleri artış gösterdi. ALA, VPA grubundaki bu seviyeleri ve artışları tersine çevirdi. Sonuç: ALA’in antioksidan özelliğini kullandığı ve VPA ile oluşturulan lens hasarını iyileştirdiği sonucuna varılmıştır.

Anahtar Kelimeler:

Valproik asit, alfa lipoik asit, lens dokusu, oksidatif stres

THE EFFECTS OF ALPHA LIPOIC ACID ON LENS INJURY IN RATS ADMINISTERED WITH VALPROIC ACID

Objective: Valproic acid (2-propyl valeric acid; VPA) is an effective short-chained fatty acid which is used for the treatment of migraine and schizophrenia. Though it provides effective treatment, its side effects are associated with free radicals and in this way it affects many organs and tissues. Alpha lipoic acid (ALA) is known to be a powerful antioxidant. Material and Methods: The aim of this current study was to investigate the protection of ALA on VPA induced lens injury. Female rats were split into four groups as follows: 1st group, control animals (corn oil per day for 15 days); 2nd group, ALA administered group (50 mg per kg each day for 15 days); 3rd group, VPA administered group (500 mg per kg each day for 15 days) and 4th group, VPA and ALA administered group to which the same dose was given at the same time each day. On the 16th day, lens tissues were taken. Results: Lens glutathione levels and glutathione-S-transferase activities were decreased while lipid peroxidation and protein carbonyl levels, superoxide dismutase, glutathione peroxidase and reductase, aldose reductase and sorbitol dehydrogenase activities were elevated after VPA administration. ALA reversed these levels and activities in the VPA group. Conclusion: We can conclude that ALA used its antioxidant property and ameliorated VPA induced lens injury.

Keywords:

Valproic acid, alpha lipoic acid, lens tissue, oxidative stress,

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