Sık Kullanılan Bazı Hücre Hatları için Kalite Kontrol: Mikoplazma Kontaminasyon Tespiti, Sitokrom B ve Sitokrom Oksidaz Alt Birim I Genlerinin DNA Dizi Analizlerinin Gerçekleştirilmesi

Amaç: Laboratuvarlarda sık kullanılan serviks epitelyal karsinom (HeLa), insan periferal kan promiyelösitik lösemi (HL-60), fare C3/bağ dokusu (L929), Madin Darby köpek böbrek (MDCK), fare nöroblastom (Neuro-2a) gibi bazı hücre hatlarının mikoplazma kontaminasyon kontrollerinin yapılması, kimlik doğrulamalarının gerçekleştirilmesi ve klonalitelerinin belirlenmesidir.

Anahtar Kelimeler:

Hücre kültürü, CYTB, COI, DNA dizi analizi, mikoplazma, Hoechst 33258

Quality Control for Some Commonly Used Cell Lines: Mycoplasma Contamination Detection, DNA Sequence Analysis of Cytochrome B and Cytochrome Oxidase Subunit I Genes

Aim: Mycoplasma contamination controls, identity verification and clonality determination of some cell lines such as cervix epithelioid carcinoma (HeLa), human peripheral blood promyelocytic leukemia (HL-60), mouse C3 / a connective tissue (L929), madin darby canine kidney (MDCK), mouse neuroblastoma (Neuro-2a) that are frequently used in laboratories.

Keywords:

Cell culture, CYTB, COI, DNA sequence analysis, mycoplasma, Hoechst 33258,

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