Bilirubin Damgalı Kitosan-Agaroz Biyomateryalinin Sentezi, Karakterizasyonu ve Bilirubin Uzaklaştırılmasında Kullanımı

Amaç: Bilirubin damgalı kitosan-agaroz biyomateryalinin; sentezi, karakterizasyonu ve bilirubin uzaklaştırılmasında kullanımının önemini tespit etmek amacıyla yapılmıştır. Yöntem: Kitosan ve agaroz doğal polimerleri materyal olarak seçilmiştir. Bilirubin damgalı kitosan-agaroz biyomateryalinin bilirubin uzaklaştırma kapasitesinin belirlenmesi için insan plazması kullanılmıştır. Bulgular: Bilirubin uzaklaştırılması için pH 7.0, sıcaklık 37oC ve başlangıç bilirubin konsantrasyonu 40 mg/g optimum şartlar olarak belirlenmiştir. NaCl konsantrasyonunun arttırılması ile bilirubin için iyonik şiddetin azaldığı tespit edilmiştir. Sonuç: Etkileşim öncesi ve sonrasında alınan serum örneklerinde bilirubin analizi gerçekleştirildi ve moleküler damgalı kitosan-agaroz biyomateryalleri ile %87’lik bir uzaklaştırma oranı elde edildi. Sulu çözeltilerden bilirubin adsorpsiyonu ile insan plazmasından bilirubin adsorpsiyonu kıyaslandığında, plazmada adsorpsiyon kapasitesinin çok az miktarda azaldığı gözlendi.

Anahtar Kelimeler:

bilirubin, kitosan-agaroz, Moleküler damga, biyomateryaller

Bilirubin Impriting Chitosan-Agarose Biomaterial Synthesis, Characterization and Use to Bilirubin Removal

Aim: This study was conducted to synthesize and characterize bilirubin imprinted chitosan-agarose biomaterial and to determine its significance on bilirubin removal. Method: Natural Chitosan and agarose polymers were chosen as materials. Human plasma was used to determine the capacity of bilirubin imprinted chitosan-agarose biomaterial for bilirubin removal. Findings: The optimum conditions for bilirubin removal were determined as pH 7.0 at 37oC with a starting concentration of 40 mg/g. An increase in NaCl concentration was shown to decrease the ionic strength for bilirubin. Conclusion: Bilirubin analysis was performed on serum samples taken before and after the interaction. A suspension rate of 87% was obtained by using molecular imprinted chitosan-agarose biomaterials. When bilirubin adsorption from aqueous solutions was compared to the adsorption of bilirubin from human plasma it was observed that the adsorption capacity of plasma was slightly decreased.

Keywords:

Molecular imprinting, bilirubin, agarose-chitosan, biomaterials,

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