Bacillus subtilis Bakterisi ile Metribuzin Herbisitinin Biyoıslahının Yapay Tarla Düzeneğinde Araştırılması

Türkiye’de domates yetiştiriciliğinde seçkin bir herbisit olan Metribuzinin (C8H14N4OS) biyoıslahı Bacillus subtilis bakterisiyle çalkalanmalı kültür koşullarında ve yapay tarla düzeneğine araştırılmıştır. Bakteri Türkiye Elazığ ilinden bir tarım arazisinden toplanan topraktan izole edilmiştir. Bölge daha önce metribuzine maruz kalmayan bir bölgedir. 1750 mg L-1 konsantrasyonda (çiftçler için tavsiye edilen konsantrasyon) 100’er mL’lik beş adet aparat hazırlanmıştır ve metribuzin her bir erlenmayer şişesine ilave edilmiştir. Her bir zenginleştirilen solüsyon × 107 Koloni oluşturan birey (KOB) içermektedir. Bu şişeler 160 rpm de 28°C’de steril koşullarda 17 gün boyunca çalkalanmıştır. 24 saatlik dilimlerde, her bir örnek toplanmıştır ve DR/890 kolorimetre ile kimyasal oksijen ihtiyacı (KOİ) kapalı refluks yöntemi ışığında belirlenirken Biyokimyasal Oksijen İhtiyacı (BOİ5) standart method 5210D ye göre oksitoplarla belirlenmiştir. Çalışma sonuçlarına göre en iyi giderim performansı 100 mL Bacillus subtilis türünde KOİ ve BOİ5 bazında 17 günde %98 oranında görülmüştür. Yapay tarla çalışmasının sonuçlarına göre KOİ ve BOİ5 parametrelerinde 2. haftanın sonunda sırasıyla %99.4 ve %96 olarak görülmüştür. Bu deneyler göstermiştir ki Bacillus subtilis alıcı ortamlardaki metribuzin gieriminde seçkin bir türdür.

Anahtar Kelimeler:

Bakteri, biyoıslah, herbisit, metribuzin

Investigation of the Bioremediation of Herbicide Metribuzin with Bacillus subtilis Bacteria at Artifical Agricultural Field

The bioremoval of metribuzin (C8H14N4OS) which is one of the selective herbicide in tomato farming in Turkey was investigated with Bacillus subtilis bacteria under agitated culture conditions and artifical agricultural field. The bacterium was isolated in collected soil samples from one of the agricultural area in Elazig province of Turkey. The area is unexposed to metribuzin before. Five apparatuses were set up and 100 mL of metribuzin in 1750 ppm concentration (advised concentration of tomato farmers) was added to each Erlenmayer flasks. 5, 10, 20, 50 and 100 mL of enriched Bacillus subtilis solutions (in sabouraud dextrose broth) added to these flasks. Each mL of the enriched solutions contains ×107 Colony forming unit (CFU). These flasks were shaken at 160 rpm at 28 0C in sterile conditions for 17 days. In 24 hours intervals, each sample was collected to flasks and chemical oxygen demand (COD) was determined with DR/890 colorimeter by the line of closed reflux method while Biochemical oxygen demand (BOD5)experiments were performed via standard methods 5210D with oxitops. As a result of the study, best removal performance observed in 100 mL Bacillus subtilis as 98% at 17 days in COD. In a soil study, 100 mL mixture of Bacillus subtilis consortia used for bioremediation studies. According to the results of artifical agricultural field study, the bioremediation seen at COD and BOD5 parameters are 99.4% and 96% respectively at the end of 2. week. This experiments have focused that, Bacillus subtilis should be a selective type for bioremediation of metribuzin on receiving environments.

Keywords:

Bacteria, bioremoval, herbicide, metribuzin,

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Alexander, M., 1999. Biodegradation and bioremediation 2th edition Academic Press, New York.
Awad, N., Sabit, H.H., Abo-Aba, SEM., Bayoumi, R.A., 2011. Isolation, characterization and fingerprinting of some chlorpyrifos-degrading bacterial strains ısolated from egyptian pesticides polluted soils. African Journal of Microbiology Research, 5(18):2855–2862.
Baczynski, T.P., Pleissner, D., Grotenhuis, T., 2010. Anaerobic biodegradation of organochlorine pesticides in contaminated soil - significance of temperature and availability. Chemosphere, 78(1):22–28.
Belal, B.E., Mohamed, F.E.N., 2013. Bioremediation of pendimethalin contaminated soil. African Journal of Microbiology Research, 7(21):2574–2588.
Bending, G.D., Lincoln, S.D., Sorensen, S.R., Morgan. J.A.W., Aamand, J., Walker, A., 2003. In-field spatial variability in the degradation of the phenyl-urea herbicide isoproturon is the result of interaction between degradative Sphingomonas spp. and soil pH. Applied and Environmental Microbiology, 69:827–834.
Beutler, E., Gelbart, T., Kuhl, W., 1990. Interference of heparin with the polymerase chain reaction. Biotechniques, 9:166.
Chowdhury, A., Pradhan, S., Saha, M., Sanyal, N., 2008. Impact of pesticides on soil microbiological parameters and possible bioremediation strategies. Indian Journal of Microbiology. 48:114–127.
Cruikshank, R., 1972. Medical Microbiology 11th, Ed. Livingstone, London.
Cycon, M., Piotrowska-Seget, Z., 2009. Changes in bacterial diversity and community structure following pesticides addition to soil estimated by cultivation technique. Ecotoxicology, 18(5):632-42.
Erguven, G.O., Yildirim, N., 2016. Efficiency of some soil bacteria for chemical oxygen demand reduction of synthetic chlorsulfuron solutions under agiated culture conditions. Cellular and Molecular Biology, 62(6):92–96.
Erguven, G.O., Yildirim, N., Adar, E., 2017. The ability of Phanerochaete chrysosporium (ME446) on chemical oxygen demand remediation in submerged culture medium supplemented with malathion insecticide. Desalination and Water Treatment, 94:231–235.
Erguven, G.O., 2017. Role of some selected fungi cultures on bioremediation of herbicide chlorsulfuron. Journal of Tekirdag Agricultural Faculty, 14(2):110–118. Kumar, M., Philip, L., 2006. Adsorption and desorption characteristics of hydrophobic pesticide endosulfan in four Indian soils. Chemosphere, 62(7):1064–1077.
Lakshmib, C.V., Kumarb, M., Khanna, S., 2008. Biotransformation of chlorpyrifos and bioremediation of contaminated soil. International Biodeterioration & Biodegradation, 62(2):204–209.
Matsumoto, E., Kawanaka, Y., Yun, S.J., Oyaizu, H., 2009. Bioremediation of the organochlorine pesticides, dieldrin and endrin, and their occurrence in the environment. Applied Microbiology and Biotechnology, 84(2):205–216.
Maya, K., Singh, R.S., Upadhyay, S.N., Dubey, S.K., 2011. Kinetic analysis reveals bacterial efficacy for biodegradation of chlorpyrifos and its hydrolyzing metabolite TCP. Process Biochemistry, 46:2130–2136.
Murthy, H.M., Manonmani, H.K., 2007. Aerobic degradation of technical hexachlorocyclohexane by a defined microbial consortium. Journal of Hazardous Materials, 149(1):18–25.
Travers, R.S., Martin, P.A.W, Reichelderfer, C.F., 1987. Selective process for efficient isolation of soil Bacillus sp. Applied and Environmental Microbiology, 53:1263–1266.
Yang, L., Li, X., Li, X, Sua, Zç, Zhanga, Cç, Zhang, H., 2014. Bioremediation of chlorimuron- ethyl contaminated soil by Hans Schlegelia sp. strain CHL1 and the changes of indigenous microbial population and N-cycling function genes during the bioremediation process. Journal of Hazardous Materials, 274:314–321.
Zelles, L., Adrian, P., Bai, Q. Y., Stepper, K., Adrian, M.V., Fischer, K., Maier, A., Ziegler, A., 1991. Microbial activity measured in soils stored under different temperature and humidity conditions, Soil Biology and Biochemistry, 23:955–962.