Nurul Aqidah SALİKAN, Nor Azimah MOHD ZAİN, Kam KAR YERN

Isolation of Antibiotic Resistant Bacteria from Rivers in Terengganu, Malaysia

Infectious disease cause by antibiotic resistant bacteria has been more difficult to treat because the ability of these bacteria to avoid the effect of antibiotic medication. Nowadays, water environment especially river has become the reservoir for the dissemination of antibiotic resistant bacteria due to antibiotic contamination derived from human activities. The exposure to antibiotic resistance bacteria in water increase the health risks associated with the waterborne bacteria in animals and humans. Therefore, this study was carried out to investigate the presence of antibiotic resistant bacteria from two rivers in Terengganu; Ibai River and Terengganu River. A total of 24 bacterial strains were isolated from both rivers. Antibiotic susceptibility of the bacteria isolates were assayed according to the Kirby-Bauer disk diffusion method using six types of antibiotics; ampicillin, rifampicin, gentamicin, tetracycline, ciprofloxacin and chloramphenicol. The results of this study indicate that 100% of bacterial strains were resistant to at least two antibiotics tested. The multiple antibiotic resistant (MAR) index of these bacteria was calculated and 23 bacterial strains show MAR index of more than 20%. These bacteria were selected for further characterization using 16S rRNA gene sequencing. Among the 23 isolates, a total of 10 bacterial strains (2A, 2B, 2C, 2I, 3A, 3B, 4A, 4C, 4D and 4E) were successfully identified as Chromobacterium violaceum, Enterobacter tabaci, Bacillus wiedmannii, Acinetobacter baumannii, Klebsiella quasipneumoniae subsp. quasipneumoniae, Chromobacterium aquaticum, Cupriavidus metallidurans, Weeksella massiliensis, Cupriavidus necator and Acinetobacter nosocomialis. The results from this study show that both of the rivers are exposed to high level of antibiotics compound which may lead the bacteria to develop mechanism of antibiotic resistant. This may cause a serious threat to the public health and environment.

Keywords:

Antibiotic resistance bacteria, multiple antibiotic resistance, river waters, Terengganu,

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1. Zaideen, I. M. M., S. Suratman, and N. M. Tahir, The Evaluation of Spatial Variation of Water Quality in Sungai Setiu Basin, Terengganu. Sains Malaysiana, 2017. 46(9): p. 1513-1520.
2. Khan, G. A., et al., Occurrence and abundance of antibiotics and resistance genes in rivers, canal and near drug formulation facilities–a study in Pakistan. PLoS One, 2013. 8(6): e62712.
3. Onuoha, S. C., The Prevalence of Antibiotic Resistant Diarrhogenic Bacterial Species in Surface Waters, South Eastern Nigeria. Ethiopian journal of health sciences, 2017. 27(4): p. 319-330.
4. Schwartz, T., et al., Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS microbiology ecology, 2003. 43(3): p. 325-335.
5. Moges, F., et al., Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia. BMC research notes, 2014. 7(1): 215.
6. Roy, R. P., M. Bahadur, and S. Barat., Isolation, identification and Antibiotic resistance of Aeromonas spp. and Salmonella spp. from the fresh water loach, Lepidocephalichthys guntea and water of Terai River Lotchka, West Bengal, India. Zoologica Poloniae, 2013. 58(1-2): p. 5-17.
7. Laith, A., and M. Najiah, Aeromonas hydrophila: antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). Journal of Aquaculture Research and Development, 2013. 5: p. 215.
8. Osundiya, O., R. Oladele, and O. Oduyebo, Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. African Journal of Clinical and Experimental Microbiology, 2013. 14(3): p. 164-168.
9. Gasim, M., et al., GIS-based analysis of water quality deterioration in the Nerus River, Kuala Terengganu, Malaysia (Vol. 112), 2018.
10. Odonkor, S. T., and K. K. Addo, Prevalence of multidrug-resistant Escherichia coli isolated from drinking water sources. International journal of microbiology, 2018. 2018: p.1-7.
11. Kathleen, M., et al., Antibiotic resistance of diverse bacteria from aquaculture in Borneo. International journal of microbiology, 2016. 2016: p.1-9.