İsmaila Yada SUDİ, Mohammed SHUAİBU, Malachi Albert TİZHE

Alpha S1-Casein Gene Polymorphısm in Nigerian Balami Sheep Breed Indigenous to Mubi

The study was conducted on five Balami sheep breed to determine and characterize the alpha S1 casein gene. Five blood samples were collected from each animal intravenously using sterile needle and syringe. The blood samples were placed in labelled tubes containing ethylene diamine tetraacetic acid (EDTA). The blood samples were transported in on ice cold chain container to the laboratory for analysis. Quick-DNA Miniprep TM kit used for DNA extraction and amplified using forward and reverse primers (CSN1S1F 5’-ACCCCTCAGGTACCCTAAGAAA-3’ and CSN1S1R 5’-GTTTATCCCCCACACTGCATTC-3’). The gene was sequenced and blasted against the NCBI database. Single nucleotide polymorphism analysis was performed to ascertain the variations. Multiple sequence alignment and phylogenetic analysis within and with the reference sequence was conducted online bioinformatics tools. Result from the analysis reveals that, the extracted DNA were found on chromosome 6, intron 16 and exon 17. The Balami breeds of sheep showed total number of polymorphic and monomorphic site of 68 and 600 respectively, and percentage of polymorphism of 10.18%. The Balami breed showed one amino acid substitution and genetic variation within breeds. Complete molecular characterization, genotyping and determination of allele frequency of alpha S1 gene in Balami breed of sheep indigenous to Nigeria and its variations is recommended for further research.

Keywords:

Balami breeds, casein gene, genetic variation,

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1. FAO, Food and Agriculture Organization. FAO Statistical Yearbook 2009. Agricultural production. (2011). http://www.fao.org/docrep/014/am079m/am079m00.html Retrieved 04.09.2010
2. Haenlein G.F.W., About the evolution of goat and sheep milk production. Small Ruminant Research. 2007. 68: p. 3-6.
3. FAO, Food and Agriculture Organization of the United Nations. Database on Agriculture.15.03, 2010. http://www.fao.org/.15.03
4. Haenlein, G.F.W., Past, present, and future perspectives of small ruminant dairy research. Journal Dairy Science, 2001. 84: p. 2097-2115.
5. Park, Y.W., and Haenlein, G.F.W., Overview of Milk of non-bovine mammals. In: Handbook of milk of non-bovine mammals (ed. by Park, Y.W., Haenlein, G.F.W.), Blackwell Publishing, Ames, Iowa, USA, 2006. p. 3-10.
6. Degen, A.A., Sheep and goat milk in pastoral societies. Small Ruminant Research, 2007. 68: p. 7-19.
7. Haenlein, G.F.W., Wendorff, W.L. and Park, Y.W., Sheep milk. Handbook of milk of non-bovine mammal.137-194. Blackwell Publishing, Ames, Iowa, USA, 2006.
8. Morand-Fehr, P., and Boyazoglu J. Present state and future outlook of the small ruminant sector. Small Ruminant Research, 1999. 34: p. 175-188.
9. Raynal-Ljutovac, K., et al., Composition of goat and sheep milk products: An update. Small Ruminant Research, 2008. 79(1): p. 57-72.
10. Park, Y.W., et al., Physico-chemical Characteristics of goat and sheep milk. Small Ruminant Research, 2007. 68: p. 88-113.
11. Storry, J.E., et al., Chemical composition and coagulation properties of renneted milks from different breeds and species of ruminants. Journal of Dairy Research, 1983. 50: p. 215-229.
12. Hinrichs, J., et al., Mediterranean milk and milk products. European Journal of Nutrition, 2004. 43 (1): p. 12-17.
13. Holt, C., and Sawyer, L., Primary and predicted secondary structures of the caseins in relation to their biological functions. Protein Engineering, 1988. 2(4): p. 251-259. Doi.10.1093/protein/2.4.251
14. Eigel, W.N., et al., Nomenclature of proteins of cow's milk: Fifth Revision. Journal of Dairy Science, 1984. 67: p. 1599-1631.
15. Threadgill, D.W., and Womack, J.E., Genomic analysis of the major bovine milk production. Protein genes. Nucleic Acids Research, 1990. 18: p. 6935-6942.
16. Lévéziel, H., et al., Restriction fragment length polymorphism of ovine casein genes: close linkage between the αs1 -, αs2 -, β- and κ-casein loci. Animal Genetics, 1991. 22: p.1-10.
17. Bevilacqua, C., et al., Translational efficiency of casein transcripts in the mammary tissue of lactating ruminants. Reproduction Nutrition Development, 2006. 46: p. 567-578.
18. DeGortari, M.J., et al., A second generation linkage map of the sheep genome. Mammal Genome, 1998. 9: p. 204-209
19. Sudi, I. Y., et al., Alpha S1 casein gene polymorphism in Ouda sheep breed of Mubi, Nigeria. Nigerian Journal of Genetics, 2019. 33(1): (in press).
20. Degerforsertr, A. M., Climate Mubi. Climate-Data.Org, 2019. http://en.climate-data.org/info/sources Retrieved on 30.09.2019.
21. Sanger, F., and Coulson, A. R., A rapid method for determining sequence in DNA by primed synthesis with DNA polymerase. Journal of Molecular Biology, 1975. 94 (3): p. 441-448
22. Corpet, F., Multiple sequence alignment with hierarchical clustering. Nucleic Acid Research, 1988. 16 (22): pp. 10881-10890.
23. Hall,,T. A., BioEdit: A user- friendly biological sequence alignment editor and analysis programmed windows 95/98nt. Nucleic Acids Symposium Series, 1999. 41: p. 95- 98.
24. Kumar, S., et al., MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 2018. 35: p.1547-1549.
25. Peakall, R., and Smouse, P. E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update. Bioinformatics, 2012. 28(19): pp. 2537-2539. Doi: 10.1093/bioinformatics/bts460
26. Edgar, R.C., MUSCLE: Muscle multiple sequence alignment with high accuracy and throughput. Nucleic acid Research, 2004. 32(5): p. 1792-97
27. Ramunno, L., G., et al., Characterization of two new alleles at the goat CSN1S2 locus. Animal Genetics, 2001. 32(5): p. 264-268.
28. Calvo, J. H., et al., Structural and functional characterization of the αs1- casein gene and association studies with milk traits in Assaf sheep breed. Livestock Science. 2013. p.1-8.
29. Chessa, S., et al., New genetic polymorphisms within ovine β- and αs2-caseins. Small Ruminant Research, 2010. 88: p. 84-88.
30. Giambra, I. J., Jäger, S., and Erhardt, G., Isoelectric focusing reveals additional casein variants in German sheep breeds. Small Ruminant Research, 2010. 90(13): p. 11-17.