Effects of some pesticides on Bombus terrestris under laboratory conditions

Pesticides have not only lethal effect on bees but they can also change their navigational behaviors. The bumblebees exposed to pesticides may not find their food and hive, and even their motor nervous system can acutely be affected. In this study, effects of some pesticides (abamectin, acetamiprid, deltamethrin, imidacloprid) on bumblebees were investigated. The bumblebee colonies were obtained from Koppert Biological Systems, Inc., Turkey. Six doses of each pesticide were tested on motor behaviors of some extremities of the bumblebees and bumblebees were fed on only 50% sucrose as a control group to compare with pesticide applications. Pesticides were applied to bees by feeding and spraying methods. Then, the situations of motor mobility of legs, antennae and proboscis extension of the bumblebees exposed to pesticides were scored. According to the results of the study, the pesticides used in the experiment had an impact on motor nervous system of the bumblebees, and the most effective pesticide was imidacloprid, followed by deltamethrin, acetamiprid, and abamectin, respectively. These results show that imidacloprid, all doses, demage basic motor coordination fundamental to locomotion and foraging and kill at label dose.

Keywords:

Bumblebees, Pesticide Imidacloprid, Proboscis extension reflex,

PDF

___

Allen-Wardell, G., Bernhardt, P., Bitner, R., Burquez, A., Buchmann, S., Cane, J., Cox, P.A., Dalton, V., Feinsinger, P., Ingram, M., Inouye, D., Jones, C.E., Kennedy, K., Kevan, P., Koopowitz, H., Medellin, R., Medellin-Morales, S., Nabhan, G.P., Pavlik, B., Tepedino, V., Torchio P., Walker, S. (1998) The Potential Consequences of Pollinator Declines on the Conservation of Biodiversity and Stability of Food Crop Yields. Conservation Biology, 12, 1, 8-17.
Aslan, M.M., Ücük, C., Candan, G. (2017) Kahramanmaraş İli Bombus Arı Türleri ve Bulundukları Bitki Örtüsünün Belirlenmesi (Determination of Bumble Bee Species and Their Foraging Plants in Kahramanmaraş Province). KSU J. Nat. Sci., 20(4): 334-338. DOI: 10.18016/ksudobil.286440
Benton, T. (2000) The bumblebees of Essex. The Nature of Essex Series, No: 4, Loginga Books, Essex, p 179
Blacquiere T, Smagghe G, Van Gestel CAM, Mommaerts V (2012) Neonicotinoids in bees: A review on concentrations, side-effects and risk assessment. Ecotoxicology 21, 973–992. DOI:10.1007/s10646-012-0863-x
Cresswell, J.E., Robert, F.L., Florance, H., Smirnoff, N. (2013) Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Manag Sci, 70, 332–337. DOI 10.1002/ps.3569.
Donovan, B.J. (1980) Interactions between native and introduced bees in New Zealand. New Zealand Journal of Ecology, 3,104-116.
Goodwin, S., Steiner, M. (1997) Introduction of Bombus terrestris for biological pollination of horticultural crops in Australia. A submission to AQIS and Environment Australia, Gosford IPM Services, Gosford.
Gösterit, A., Gürel, F. (2005) Bombus terrestris (HYMENOPTERA: APIDAE) arilarinin Yayılmasının ekosistem üzerine etkileri (Effects Of Invasion Of Bombus terrestris (Hymenoptera: Apidae) On The Ecosystem) Uludag Bee Journal, 5, 115-121.
Gösterit. A., Gürel, F. (2010) Bombus Arıları ve Bitkisel Üretim Açısından Önemleri. Arıcılık Arastırma Dergisi, 4, 9-12.
Gürel, F., Gösterit, A., Talay, R., Efendi, Y. (2001) Bombus arısı (Bombus terrestris)’nın örtüaltı yetiştiricilikte ve ekolojik tarımda kullanımı. Türkiye 2. Ekolojik Tarım Sempozyumu, 14-16 Kasım 2001, Antalya, 245-255.
Kandemir, İ. (2007) Amerika Birleşik Devletleri’nde toplu arı .lümleri ve koloni çökme bozukluğu (KÇB) üzerine bir derleme. Uludağ Arıcılık Dergisi, 7, 63-69.
Karahan, A., Çakmak, İ., Hranitz, J.M., Karaca, İ., Wells, H. (2015) Sub-lethal imidacloprid effects on honey bee flower choices when foraging. Ecotoxicology, 24, 6, 1-9. DOI 10.1007/s10646-015-1537-2
Laycock, I., Lenthall, K.M., Barratt, A.T., Cresswell, J.E. (2012) Effects of imidacloprid, a neonicotinoid pesticide, on reproduction in worker bumblebees (Bombus terrestris). Ecotoxicology, 21, 1937–1945.
Michener, C.D. (1979) Biogeography of the bees. Annals of the Missouri Botanical Garden. 66, 3, 277-347.
Michener, C.D. (2007) The Bees of the World. The Johns Hopkins University Press, Baltimore and London. p 953.
Özbek, H., (2010) Arılar ve insektisitler. İnsektisitlerin Arılara Olumsuz Etkileri. Uludağ Arıcılık Dergisi, 10, 3, 85-95.
Williams, P.H. (1998) An annotated checklist of bumblebees with an analysis of patterns of description. Bulletin of the Natural History Museum Entomology Series, 67, 79-152.
Woodcock, B.A., Isaac, N.J.B., Bullock, J.M. Roy, D.B., Garthwaite, D.G.Crowe, A., Pywell, R.F. (2016). Impacts of neonicotinoid use on long-term population changes in wild bees in England. Nature Communications, 7, 12459, 1-8. DOI: 10.1038/ncomms12459.

International Journal of Agriculture Environment and Food Sciences-Cover

Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 2017
Yayıncı: Gültekin ÖZDEMİR

Arşiv