Mustafa ULAS

Effect of repaglinid on ovariectomy induced bone loss in rats: comparison with 17β-estradiol

Aim: The aim of this study was to investigate the effect of repaglinide (RPG), anti-diabetic drug used commonly diabetic patients, on bone loss ovariectomized (OVX) rats”.Material and Methods: Forty Wistar albino rats were equally divided into five groups as follows: Group 1, control, non-OVX rats; Group 2, OVX rats administered 0.1 % ethanol; Group 3, OVX rats administered subcutan 17β-estradiol (E2) at a dose of 40 µg/kg; Group 4, OVX rats administered oral RPG at a dose of 0.5 mg/kg; Group 5, OVX rats administered E2 (40 µg/kg) and RPG (0.5 mg/kg) rats. Administration of drugs started 5 days after bilateral ovariectomy and continued for 35 days. After 35 days of treatment, the animals were sacrificed and the whole femurs were dissected and were analyzed for bone mineral density (BMD) and bone mineral content (BMC) by dual-energy x-ray absorptiometry (DXA).Results: There were no significant difference between OVX and RPG groups for BMC and BMD values. Compared with the OVX groups, E2 administration of OVX groups had higher BMC but no significant difference for BMD. OVX group had significantly lower value than the control group.Conclusion: RPG administrated to OVX rats for 35 days had no beneficial effect on bone loss status in OVX rats.

PDF

___

Vierte B, Güttner J. Effects of the oral antidiabetic repaglinide on the reproduction of rats Arzneimittelforschung 2000;50(5):425-40.
Chakraborty PK, Brown JL,Ruff CB, Nelson MF, Mitchell AS Effects of long-term treatment with estradiol or clomiphene citrate on bone maintenance, and pituitary and uterine weights in ovariectomized rats. J Steroid Biochem Mol Biol 1991(40(4-6:725-9.
Manolagas SC, O’Brien CA, Almeida M. The role of estrogen and androgen receptors in bone health and disease. Nat Rev Endocrinol 2013;9(12):699-712.
Oursler MJ. Direct and indirect effects of estrogen on osteoclasts J Musculoskel Neuron Interact 2003;3(4):363-6.
Çelik O, Hasçalık S, Tamser M, Türköz Y, Kekilli E, Yağmır C, Boz M. Enfluence of resveratrol against ovariectomy induced bone loss in rats: comparison with conjugated equine estrogen tibolone and raloxifene. Gynecol Obstet Reprod Med 2007;(13):292-9.
Johnston BD, Ward WE. The Ovariectomized Rat as a model for studying alveolar bone loss in postmenopausal women. Biomed Res Int 2015;2015:635023.
Kim MH, Lee JE, Lee JS, Yang WM. Improvement of osteoporosis by lycium chinense administration in ovariectomized mice J Chin Med Assoc 2017;80(4):222-6.
B.Raczyńska, G.Raczyński, J.Malczewska, B.Szczepańska, A. Czeczuk Bone mineral content (BMC) and bone mineral density (BMD) in postmenopausal women formerly practising kayaking and fencing Biology of Sport 2003;(20)2:148-58.
Jeney V, Clinical impact and cellular mechanisms of iron overload-associated bone loss Front Pharmacol 2017;(21):77
Manhan LK, Escott-Stump S Krause’s food nutrition and diet therapy. In: Anderson JJB ed. Nutrition and bone health; 2004. p.645-7.
Chen HH, Horng MH, Yeh SY, Lin IC, Yeh CJ, Muo CH, et al. glycemic control with thiazolidinedione is associated with fracture of T2DM patients. PLoS One 2015;10(8):1-9.
Arama C, Nicolescu C, Nedelcu A, Monciu CM. Synthesis and characterization of the inclusion complex between repaglinide and sulfobutylether-b-cyclodextrin (Captisol) J Incl Phenom Macrocycl Chem 2011;(70):421-8.
Dittfeld A, Koszowska A, Brończyk AP, Nowak J, Gwizdek K, Zubelewicz-Szkodzińska B. Phytoestrogens-whether can they be an alternative to hormone replacement therapy for women during menopause period? Wiad Lek 2015;68(2):163-7.
Celik O, Erdem G, Hascalik S, Karakas HM, Tamser M. Magnetic resonance spectroscopic comparison of the effects of resveratrol (3,4’,5-trihydroxy stilbene) to conjugated equine estrogen, tibolone and raloxifene on ovariectomized rat brains.Eur J Obstet Gynecol Reprod Biol 2005;120(1):73-9.
Hascalik S, Celik O, Tamser M, Mizrak B Effects of resveratrol, raloxifene, tibolone and conjugated equine estrogen on vaginal squamous cell maturation of ovariectomized rats. Gynecol Obstet Invest 2005;60(4):186-91.
Ulas M, Cay M. 17β-Estradiol and vitamin E modulates oxidative stress-induced kidney toxicity in diabetic ovariectomized rat. Biol Trace Elem Res 2011;144(1-3):821-31.
Ulas M, Cay M. Effects of 17β-estradiol and vitamin E treatments on blood trace element and antioxidant enzyme levels in ovariectomized rats. Biol Trace Elem Res 2011;139(3)(3):347-55.
Bhardwaj A, Swe KM, Sinha NK, Osunkwo I. Treatment for osteoporosis in people with ß-thalassaemia. Cochrane Database Syst Rev 2016;(3):CD010429.
Laba TL. using discrete choice experiment to elicit patient preferences for osteoporosis drug treatments: where to from here? Arthritis Res Ther 2014;16(2):106.