Bay Aysun KARABULUT, Ömer AKYOL, İsmail TEMEL, Elif ÖZEROL, Engin M. GÖZÜKARA

Yaş ve sigara içiminin eritrosit katalaz aktivitesi ve bazı hematolojik parametreler üzerine etkisi

Katalaz (CAT, EC 1. 11.1.6), hidrojen peroksitin suya dönüşümünü katalizleyen antioksidan bir enzimdir. Dokulardaki katalaz aktivitesi büyük oranda değişiklik gösterir. Enzim _insan eritrositleri katalaz bakımından zengindir. Bu çalışmada, bazı şikayetlerle farklı kliniklere başvuran 475 sağlıklı bireyden ıııııııenzim aktivitesine etkisini-için sağlıklı bireyler sigara içenler (n=88) ve sigara içmeyenler (n=activities between the groups were not statistically significant, CAT activities were increased by age. There is no effect of smoking on CAT activity in erytrocytes. Theseen, smokers have higher CAT activity than nonsmokers, but it was not statistically 386) olmak üzere iki gruba ayrıldıu88gruplarındaki katalaz aktivitelerini belirlemek için, ---81 arasında olanlar (n=00eritrosit CAT aktiviteleri sırasıyla, 2981 ± 962 k/grHb, 3039 ± 923 k/grHb ve 3086±797, 3266 ±13 k/grHb __olarak anlamlı bk fark gözlenmedi. Sigara içiminin eritrosit katalaz aktivitesine etkisi olmadığı görüldü. Sigara içenlerde katalaz aktivitesi 3173 ± 910 k/gHb iken, sigara iç_nlerde 3065 ± 979 k/gHb 00fakat bu istatistiki olarak anlamlı değildi.

The effect of age and smoking on erythrocyte catalase activity and some hematological parameters

Catalase (CAT, EC 1.11.1.6) is an antioxidant enzyme that catalyses the conversion of hydrogen peroxide (H2O2) to water and 1/2 O2. The catalase shows various catalitic activity in different tissues. The enzyme activity is higher in liver and kidney and the lower in connective tissues. Human erythrocytes are rich in ctyse. _----attended to different clinics for different complaints. We have also divided the subjects into smoker (n=88) and nonsmoker groups (n=386) in order to ***?smoking may affect the enzyme activity or not in erythrocytes. Enzyme activity was determined according to the method of Aebi. Activity was expressed in rate constant per gram hemoglobin (k/grHb). Besides CAT activity; RBC, MCV, MCHC, MCH and HCT values were also obtained from Coulter autosampler. In order to determine CAT activity in different age groups, subjects were divided into to four groups as follows: Group 1: ages between 0-20 (n=28), Group 2: ages between 21-40 (n=192). Group 3: ages between 41- 60 (n-172). Group 4: ages between 61-80 (n=83) Erythrocyte CAT activities of these groups were found to be 2981 ± 962 k/gHb, 3039 ± 923 k/gHb, 3086±797 k/gHb and 3266 ±13 k/gHb, respectively. Although differences of the mean activities between the groups were not statistically significant, CAT activities were increased by age. There is no effect of smoking on CAT activity in erytrocytes. Theseen, smokers have higher CAT activity than nonsmokers, but it was not statistically significant.

PDF

___

1. Nonhebel DC, Walton JC: Free Radical Chemistry. Cambridge, MA, University Press,1974
2. Wei YH, Lu CY, Wei CY, Ma YS, Lee HO 11 Oxidative stress in human aging and itochondrial disease-consequences of defective mitochondrial respiration and impaired antioxidant enzyme system. Chin J Physiol 2001; 44 (1):1- 3. (l):44-55
4. Denham Harman: Aging and Oxidative Stress. JIFCC 1998; 10:24-27
5. Bolzan A D; Bianchi B. S; Bianchi NO: Superoxide dismutase, catalase and glutathione
6. Halliwell B, Aeschbach R, Loliger J, et al:The characterization of antioxidants. Food Chem Toxicol, 1995; 33: 601-607
7. Andersen H. R., Nielsen J.B., Nielsen F and Grandjean P: Antioxidative enzyme activities in human erytrocytes. Clin Chem. 1997;43:(4) 562-568
8. Kurata M. ; Suzuki M.; Ağar NS: Antioxidant systems and erythrocyte life span in mammals. Comp Biochem Physiol 1993 ; 106 (3): 477-78
9. Harman D; Aging: Prospects for further increases in the functional life span. Age 1994;17:119-146.
10. Harman D; Aging and disease: Extending the functional life span. Ann NY Acad Sci 1996:786:2312-36.
11. Harman D:Free radical invovement in aging: Pathophsiology and threrapeutic implications. Drugs &Aging 1993;3:60-80
12. Agar NS, Sadrzadeh SM, Hallaway PE, Eaton JW: Erythrocyte cataJase.A somatic oxidant defense?] Clin Invest 1986;77(1):319-21
13. Akkuş I Serbest Radikaller ve Fizyopatolojik etkilcri.Mimoza basım, Konya 1995: 60- 61
14. Guemouri L, Artur Y, Herbeth B, Jeandel C, Cuny G, Siest G: Biological variabilty of superoxide dismutase, glutathione peroxidase, and catalase in blood. Clin Chem 1991; 37:1932-7
15. Jozwiak Z, Jasnowska B: Changes in oxygen-Metabolizing enzymes and lipid peroxidation in human erytrocytes as a function of age of donor. Mech Ageing Dev 1985;32:77-83 16.