Hülya UZKESER, Nilnur EYERCİ, Saliha KARATAY, Kadir YILDIRIM, Eda DİYARBAKIR, Atilla TOPÇU, İbrahim PİRİM

The distribution of HLA B27 in patients with ankylosing spondylitis in eastern anatolia

Ankilozan spondilit (AS) etyolojisi bilinmeyen kronik enflamatuvar bir hastalıktır. Patogenezde genetik ve çevresel faktörlerin önemli rol oynadığı bilinmektedir. AS gelişimi ile HLA B27 arasında güçlü bir birliktelik saptanmıştır. Bu çalışma ile daha önceki literatür bilgileri ışığında bölgemizdeki HLA B27-AS ilişkisi karşılaştırıldı. Bu çalışmada, modifiye New York kritelerine göre sınıflandırılmış AS’li hasta ve sağlıklı kontroller üzerinde HLA B27 frekansı araştırılmıştır. Çalışmaya birbirleri ile akraba olmayan 84 AS’li hasta ile 73 gönüllü kemik iliği donörü dahil edildi. HLA B27 tiplendirilmesi PCRSSP yöntemi ile belirlendi. Elde edilen bulgulara göre çalışmaya alınan AS’li hastaların yaş ortalaması 31±11yıl olarak bulundu. Aynı grupta erkeklerde (%39.3) HLA B27 frekansı kadınlardan (%19.0) çok daha yüksekti. Ankilozan spondilitli hastalar (%58,3) ile kontrol grubu (%4.3) karşılaştırıldığında hasta grubunda HLA B27 pozitifliğinin yüksek olduğu görüldü (p

Doğu Anadolu Bölgesinde ankilozan spondilitli hastalarda HLA B27 frekansının dağılımı

Ankylosing spondylitis (AS) is a chronic inflammatory disease with unknown etiology. Genetic and environmental factors are known to play important roles in the pathogenesis of the disease. A strong association has been detected between AS development and HLA B27. In this study, relationship between HLA B27 and AS in our region has been investigated in the light of literature. In this study, distribution of HLA B27 frequency has been investigated in AS patients classified according to modified New York criteria and in healthy controls. Sequence specific primer polymerase chain reaction (SSP-PCR) technique was used for HLA B27 typing unrelated 84 AS patients and 73 bone marrow volunteer donors. Mean age of AS patients were 31±11 years. In the same group, HLA B27 frequency was found higher in males (39.3%) compared to females (19.0%). HLA B27 positivity was found significantly higher in AS patients (58.3%) when compared the control group (4.3%) (p

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1. Thorsby E. Invited Anniversary Review: HLA Associated Diseases. Hum Immunol 1997; 53: 1-11. DOI: 10.1016/S0198-8859(97)00024-4
2. Khan MA. HLA-B27 and Its Pathogenic Role. J Clin Rheumatol 2008; 14: 50-2. DOI: 10.1097/RHU.0b013e3181637a38
3. Weisman M, Van der Heijde D, Reveille J. Ankylosing Spondylitis and Spondyloathropathies. London: 2008; 10: 121-8.
4. Onen F, Akar S, Birlik M, et al. Prevalence of Ankylosing Spondylitis and Related Spondyloarthritides in an Urban Area of Izmir, Turkey. J Rheumatol 2008; 35: 305-9.
5. Bakland G, Nossent HC, Gran JT. Incidence and Prevalence of Ankylosing Spondylitis in Northern Norway. Arthritis Rheum 2005; 53: 850-5. DOI: 10.1002/art.21577
6. Bodur H, Ataman S, Bugdaycı DS, et al. Description of the Registry of Patients with Ankylosing Spondylitis in Turkey: TRASD-IP. Rheumatol Int 2012; 32:169-76. DOI: 10.1007/s00296-010-1599-7
7. Birinci A, Bilgici A, Kuru O et al. HLA-B27 Polymorphism in Turkish Patients with Ankylosing Spondylitis. Rheumatol Int 2006; 2: 285-7. DOI: 10.1007/s00296-005-0614-x
8. Arnett FC. Ankylosing spondylitis. In: Koopman WJ, (ed).Arthritis and Allied Conditions.A Texbook of Rheumotology. William and Wilkins 1997; 1197-208.
9. Low frequency of HLA-B27 in Ankylosing Spondylitis Patients from Turkey. Joint Bone Spine 2008; 75: 299- 302. DOI: 10.1016/j.jbspin.2007.06.021
10. Bodur H, Ataman S, Bulut L, et al. Characteristics and Medical Management of Patients with Rheumatoid Arthritis and Ankylosing Spondylitis. Clin Rheumatol 2008; 27: 1119-25. DOI: 10.1007/s10067-008-0877-1
11. Ozgocmen S, Ardicoglu O, Kamanlı A, et al. Pattern of Disease Onset, Diagnostic Delay, and Clinical Features in Juvenile Onset and Adult Onset Ankylosing Spondylitis. J Rheumatol 2009; 36: 2830-3. DOI: 10.3899/jrheum.090435
12. Khan MA, Ball EJ. Genetic Aspects of Ankylosing Spondylitis. Best Pract Res Clin Rheumatol 2002; 16: 675- 90.
13. Feldtkeller E, Khan MA, Van der Heijde D, et al. Age at Disease Onset and Diagnosis Delay in HLA-B27 Negative vs. Positive Patients with Ankylosing Spondylitis. Rheumatol Int 2003; 23: 61-6.
14. Bhatia K, Prasad ML, Barnish G, et al. Antigen and Haplotype Frequencies at 3 Human-Leukocyte Antigen Loci (HLA-A, HLA-B, HLA-C) in the Pawaia of Papua- New-Guinea. Am J Physical Anthropol 1988; 75: 329-40. DOI: 10.1002/ajpa.1330750304
15. Gul A, Uyar FA, Inanc M, et al. A Weak Association of HLA-B*2702 with Behcet's Disease. Genes Immun 2002; 3: 368-72.
16. Sieper J, Rudwaleit M, Khan MA, et al. Concepts and Epidemiology of Spondyloarthritis. Best Pract Res Clin Rheumatol 2006; 20: 401-17. DOI: 10.1016/j.berh.2006.02.001
17. Chen HH, Chen TJ, Chen YM, et al. Gender Differences in Ankylosing Spondylitis-Associated Cumulative Healthcare Utilization: A Population-Based Cohort Study. Clinics (Sao Paulo) 2001; 66: 251-4. DOI: 10.1590/S1807- 59322011000200012