Üzümsü Meyvelerde Melatonin İçeriği ve Antioksidan Kapasitenin İncelenmesi

Bu çalışmada üzümsü meyvelerden; kurt üzümü (Lycium barbarum), horum dutu (Morus nigra), beyaz dut (Morus alba), böğürtlen (Rubus fructicous L.), mor dut (Morus rubra) ve Bursa siyah dutunda melatonin varlığı durumu HPLC-FD sistemi kullanılarak belirlendi. Tüm örneklerde farklı miktarlarda melatonin tespit edildi. Ayrıca meyve örneklerinde toplam fenolik içerik ve antioksidan kapasite çalışıldı. Antioksidan kapasiteyi belirlemek için DPPH ve ABTS testleri uygulandı. Meyve örneklerindeki melatonin miktarının 123.44 ile1600.48 ng/gFW arasında değiştiği görüldü. Kurt üzümünde (BF1) bulunan melatonin derişiminin diğer üzümsü meyvelere göre daha yüksek olduğu bulundu. En yüksek toplam fenolik içerik ve antioksidan kapasite mor duttan (BF6) sırasıyla 276.83 mg GAE/100 gFW, 452.38 mg TE/100 gFW (DPPH) ve 555.73 mg TE/100 gFW (ABTS) olarak elde edildi. Sonuçlar, toplam fenolik içerik ile antioksidan kapasite arasında pozitif yönde anlamlı bir korelasyon olduğunu göstermektedir.

Anahtar Kelimeler:

Antioksidan kapasite, Üzümsü meyveler, Melatonin

INVESTIGATION OF MELATONIN CONTENT AND ANTIOXIDANT CAPACITY IN GRAPE BERRIES

In this study, six berry fruits; goji berry/wolfberry (Lycium barbarum), black mulberry (Morus nigra), white mulberry (Morus alba), blackberry (Rubus fructicous L.), purple mulberry (Morus rubra) and Bursa Black mulberry (unknown) were analyzed by HPLC-FD to determine whether they present melatonin. The melatonin was detected different amounts in all samples. Besides, the total phenolic content and antioxidant capacity were studied in all fruit samples. DPPH and ABTS tests were applied to determine the antioxidant capacity. It was observed that the amount of melatonin in the fruit samples varied between 123.44 and 1600.48 ng/gFW. It was found that the concentration of melatonin in goji berry (BF1) was higher than other berry fruits. The highest total phenolic content and antioxidant capacity to were obtained from purple mulberry (BF6) as 276.83 mg GAE/100 gFW, 452.38 mg TE/100 gFW (DPPH) and 555.73 mg TE/100 gFW (ABTS), respectively. Results reveals a significant positive correlation between the total phenolic content with antioxidant capacity.

Keywords:

Antioxidant capacity, Berry fruits, Melatonin,

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