İsmail POYRAZ, Berru ŞAHİN, Evren ATMACA

SCAR Markörler Kullanarak On İki Yerel Fasulye Çeşidinde P. Syringae Pv. Phaseolicola ve X. Axonopodis Pv. Phaseoli’ye Karşı On Direnç Geninin Tespit Edilmesi

Fasulye (Phaseolus vulgaris), dünya genelinde önemli bir besin ve vitamin kaynağıdır. Farklı kültürlerdefarklı yemek formları olarak tercih edilmektedir. Bazı bakteriyel ve fungal hastalıklar, fasulye üretiminde ciddikayıplara neden olmakta ve beslenme ve ekonomiyi tehdit etmektedir. Bu çalışmada, on iki yerel fasulye çeşidinde(Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal, Beryl, Yunus-90, Göynük-98, Önceler-98,Eskişehir-855), iki bitki hastalığına (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv.phaseoli) karşı bazı direnç genleri belirlenmesini amaçlanmıştır. P. syringae pv. phaseolicola için dört direnç geni(SR13, ST8, SH11 ve SB10) ve X. axonopodis pv. phaseoli için ise altı (SAP6, BAC6, SU91, BC420, R7313 veR4864) direnç geni taranmıştır. Her bir direnç geni için polimeraz zincir reaksiyonu (PCR)-çoğaltımları spesifkSCAR belirteçler ile gerçekleştirilmiştir. Direnç genlerinin tespiti için elde edilen DNA bantları, var veya yok olarakkaydedilmiştir. Karşılaştırma yapmak için, iki bitki hastalığına karşı hastalık yapma oranları tarla verilerinden eldeedilmiştir. Özellikle, P. syringae pv. phaseolicola’ya karşı dört direnç geni içeren iki fasulye türünün (Aslan veBeryl), tarlada bu hastalığa karşı daha dirençli olduğunu gözlenmiştir. On iki fasulye çeşidindeki tüm direnç genleriiçin analiz sonuçlarını ortaya konulmuş ve tarladaki direnç oranları ile karşılaştırılmıştır. Bu çalışmadan elde edilenveriler, fasulye üretiminde patojenlere karşı dirençli çeşitlerin geliştirilmesine ciddi bir katkı sağlayacaktır

Anahtar Kelimeler:

Bitki Hastalıkları, direnç gen belirteçleri, pcr, phaseolus vulgaris

Detection of Ten Resistance Genes Against P. syringae pv. phaseolicola and X. axonopodis pv. phaseoli in Twelve Local Bean Varieties Using SCAR Markers

Bean (Phaseolus vulgaris) is an important food and source of vitamin worldwide. It is preferredin different cooking forms in different cultures. Some bacterial and fungal diseases cause serious loss in beanproduction and impend nutrition and economy. In this study, we aimed to investigate the presence of someresistance genes against two plant diseases (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodispv. phaseoli) in twelve local bean varieties (Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal,Beryl, Yunus-90, Göynük-98, Önceler-98, Eskişehir-855). We scanned four resistance genes (SR13, ST8, SH11and SB10) for P. syringae pv. phaseolicola and six resistance genes (SAP6, BAC6, SU91, BC420, R7313 andR4864) for X. axonopodis pv. phaseoli. PCR amplifcations were performed with specifc SCAR markers for eachresistance gene. The obtained DNA bands were scored as present or absent for the detection of resistance genes.For comparison, the virulence rates of two plant diseases against twelve bean varieties were obtained from feldresults. We especially observed that two bean varieties (Aslan and Beryl) including four resistance genes against P.syringae pv. phaseolicola are more resistant to this disease in feld. We revealed analysis results for all resistancegenes in twelve bean varieties and compared with resistance rates in feld. The data obtained from this study willseriously be conduce to the improvement of resistant varieties against pathogens in bean production

Keywords:

PCR, phaseolus vulgaris, plant diseases, resistance gene markers,

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