Gıda Katkı Maddesi Olan Sodyum Sülftin Tubifex tubifex’te Katalaz ve Glutatyon–S-Transferaz Enzim Aktivitelerine Etkileri

Sodyum sülft gıda ürünlerinin taze görünümünü korumak için gıda endüstrisi tarafından kullanılır. Buçalışmada, sodyum sulft sublethal dozunun suda yaşayan oligochaeta grubundan Tubifex tubifex üzerinde etkileriaraştırılmış, Katalaz ve Glutatyon–S-Transferaz enzim aktivite değişiklikleri değerlendirilmiştir. Antioksidan enzimaktivite değişiklikleri, Tubifex tubifex’te farklı sodyum sülft konsantrasyonlarında (2.5mM, 5mM ve 10mM) 2, 4ve 6 saat maruz bırakılmasının bir sonucu olarak araştırılmıştır. Katalaz ve Glutatyon–S-Transferaz aktivitesinin,veriler kontrol gruplarıyla karşılaştırıldığında zamana bağlı arttığı gözlenmişti

Effects of Sodium Sulfte as a Food Additive on Catalase and Glutathione-S-Transferase Enzyme Activities in Tubifex tubifex

Sodium sulfte is used to keep the food product with fresh appaerence by food industry. In this study, the effects ofsodium sulfte sublethal dose on aquatic oligochaeta Tubifex tubifex were investigated and enzyme activity changes of Catalaseand Glutathione-S-Transferase were evaluated. The antioxidant enzyme activity changes were searched as a result of 2, 4 and6 hour exposure within various concentrations (2.5mM, 5mM and 10mM) of sodium sulfte in Tubifex tubifex. Catalase andGlutathione-S-Transferase activity increased depending on the time when data were compared with the control groups

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  • Altuğ T, 2001. Gıda Katkı Maddeleri. Meta Basım Matbaacılık, İzmir, Turkey. 286p.
  • Atman ÜC, 2004. Gıda Katkı Maddeleri ve Gıda Kontrolü. Sürekli Tıp Eğitim Dergisi (Sted) 13 (3): 86-88.
  • Armstrong RN, 1997. Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem. Res. Toxicol, 10: 2-18.
  • Boğa A, Binokay S, 2010. Gıda Katkı Maddeleri ve Sağlığımıza Etkileri. Arşiv 19: 14.
  • Bradford MM, 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry. 72: 248-254.
  • Claiborne A, 1985: Catalase activity. In: Handbook of Methods for Oxygene Radical Research. 283-284.
  • Chapman PM, Farrell MA, Brinkhurst RO, 1982: Relative tolerances of selected aquatic oligochaetes to individual pollutants and environmental factors. Aquat. Toxicol, 2: 47.
  • Çalışır EZ, Çalışkan D, 2003. Gıda Katkı Maddeleri ve İnsan Sağlığı Üzerine Etkileri. Ankara Ecz. Fak. Dergisi 32 (3):193-206.
  • Eaton DL, Bammler TK, 1999. Concise Review of the Glutathione S-Transferases and their Significance to Toxicology. Toxicological Sciences, 49: 156-164.
  • EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS), 2010. Scientific Opinion on the use of potassium sulphate and sodium sulphate as sources of respectively potassium and sodium added for nutritional purposes to food supplements. EFSA Journal 8(12): 1940
  • Fazio T, Warner CR, 1990. A review of sulphites in foods: analytical methodology and reported findings. Food Addit Contam. 7(4): 433-54.
  • Günes FE, 2014. Sülfitler ve Gıda Katkı Maddesi Olarak Kullanılması. Akademik Gıda 12(2): 114-119.
  • Kayraldız A, Kaya FF, Canımoğlu S, Rencüzeoğulları E, 2006 Mutagenicity of five food additives in Ames/Salmonella/microsome test. Annals of Microbiology. 56 (2): 129-133.
  • Przybilla B, Ring J, 1987. Sulfite hypersensitivity. Hautarzt 38(8): 445-8.
  • Rathore RS, Khangarot BS, 2003: Effects of Water Hardness and Metal Concentration on a Freshwater Tubifex Tubifex Muller. Water, Air, and Soil Pollution. 142 (1): 341-356.
  • Regoli F, Principato G,Bertolli E, 1997.Biochemical characterization of the antioxidant system in the scallop Adamissium colbeki, a sentinel organism for monitoring the Antarctic environment. Polar Biology.17, 251-258.
  • Switala J, Loewen PC, 2002. Diversity of properties among catalases. Archives of Biochemistry and Biophysics. 401(2): 145–154.
  • Taylor SL, Higley NA, Bush RK, 1986. Sulfites in foods: uses, analytical methods, residues, fate, exposure assessment, metabolism, toxicity, and hypersensitivity. Adv Food Res 30: 1-76.