Gama ile Işınlanmış Klorpropamid ve Prokainamid Hidroklorür İlaç Hammaddelerinde Oluşan Serbest Radikallerin EPR Spektroskopisi ile Tanımlanması

Bu çalışmada tıpta yaygın kullanılan klorpropamid ve prokainamid hidroklorür ilaç hammaddelerinde gama ile ışınlanarak oluşan serbest radikallerin yapısal özelliklerinin elektron paramanyetik rezonans spektroskopisi kullanılarak belirlenmesi amaçlanmıştır. Gama ile ışınlama sonucunda hem klorpropamid ve hem de prokainamid hidroklorür numunelerinde iki farklı radikal oluştuğu tespit edilmiştir. Klorpropamid ve prokainamid hidroklorür numunelerinde sırasıyla -NĊH-, -NCH2ĊH2- ve –NĊH-, -NĊHCH- radikallerinin oluştuğu önerilmiştir. Toz formunda çalışılan örneklerin oda sıcaklığında kaydedilmiş deneysel spektrumlarına en yakın simüle spektrumları simülasyon yazılımı kullanılarak elde edilmiştir. Önerilen radikallerin deneysel spektrumlara olan katkıları hesaplanmıştır. Elde edilen radikallerin g değerleri ve eşlenmemiş elektronların aşırı ince yapı sabitleri hesaplanmıştır.

Anahtar Kelimeler:

Elektron paramanyetik rezonans, Gama ışınlaması, İlaç hammaddesi

Identification of Free Radicals Formed in Gamma-Irradiated Chlorpropamide and Procainamide Hydrochloride Pharmaceutical Raw Materials by EPR Spectroscopy

In this study, it was aimed to determine the structural properties of free radicals formed by gamma irradiation in chlorpropamide and procainamide hydrochloride drug raw materials, which are widely used in medicine, by using electron paramagnetic resonance spectroscopy. As a result of gamma irradiation, it was determined that two different radicals were formed in both chlorpropamide and procainamide hydrochloride samples. It was suggested that –NĊH-, -NCH2ĊH2- and –NĊH-, -NĊHCH2- radicals were formed in chlorpropamide and procainamide hydrochloride samples, respectively. The simulated spectra closest to the experimental spectra recorded at room temperature of the samples studied in powder form were obtained using simulation software. The contributions of the proposed radicals to the experimental spectra were calculated. The g values of the obtained radicals and the hyperfine structure constants of the unpaired electrons were calculated.

Keywords:

Electron paramagnetic resonance, Gamma irradiation, Pharmaceutical raw material,

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