İbrahim ÖZKOÇ, Mustafa BİLGİ, Cem Tolga GÜRKANLI, Luau MUSTAFA

Bazı Orkide Türlerinde Ekim Öncesi Tohum Uygulamaları ve Bu Uygulamaların Tohum Çimlenme ve Gelişmesine Olan Etkisi

Bitkilerin gelişimi söz konusu olduğunda öncelikle ilk aşama bitki tohumunun sağlıklı bir şekilde çimlenmesidir. Bu durumun gerçekleşmesi orkideler söz konusu olduğunda diğer bitki türlerine göre daha zor olabilmektedir. Bu çalışmamızda elimizde bulunan orkide tohumlarının in-vitro olarak çimlenmesi gözlemlenmiştir. Sıcaklık, kuraklık, Ph gibi abiyotik faktörler, tohum çimlenmesine karşı stres oluşturabilmektedir ve tohumun çimlenmesini engelleyici etkiye sahip olmaktadır. Bu durumu engelleyebilmek için tohumların bazı yöntemlerle biyolojik ajanlarla kaplanması sağlanmıştır. Bu çalışmamızda, daha öneceki çalışmalarımızda izole ettiğimiz endofitik bakteri ve fungal izolatlar kullanılarak, laboratuvar ortamında tohumlar biyopriming uygulamalara tabii tutularak kaplanmış ve orkide tohumlarının çimlenmelerine olumlu bir etkisi olup olmadığı gözlemlenmiştir. Yapılan değerlendirmede özellikle hem endofitik fungal ve bakteri örneklerini içeren karışımların en etkili işlemler olduğu görülmüştür. Bu çalışma orkidelerde hem fungal hem de bakteriyel probiyotik denemesinin ilk örneğini oluşturmaktadır.

Anahtar Kelimeler:

PGPR, Bio-priming, Çimlenme, Endofitik Bakteri ve Fungus

Pre-Sowıng Seed Applications in Some Orchid Species and The Effect of These Applications on Seed Germination and Development

When it comes to the development of plants, the first step is the healthy germination of the plant seed. The realization of this situation can be more difficult when it comes to orchids compared to other plant species. In this study, in-vitro germination of orchid seeds was observed. Abiotic factors such as temperature, drought and Ph can create stress against seed germination and have an inhibitory effect on seed germination. In order to prevent this situation, the seeds are covered with biological agents by some methods. In this study, by using endophytic bacteria and fungal isolates isolated in our previous studies, the seeds were covered with biopriming applications in the laboratory and it was observed whether there was a positive effect on the germination of orchid seeds. In the evaluation, it was observed that the mixtures containing both endophytic fungal and bacterial samples were the most effective treatments. This study constitutes the first example of both fungal and bacterial probiotic testing in orchids.

Keywords:

PGPR Bio-priming, Germination, Endophytic Bacteria and, Fungi,

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