Gadoterik Asit’in In Vitro Koşullarda Kardeş Kromatid Değişimi ve Mitotik İndeks ÜzerineEtkisinin Değerlendirilmesi

Gadoterik asit manyetik rezonans (MR) görüntülemede teşhis amaçlı kullanılan ekstraselülergadolinyumlu kontrast maddedir. Bu çalışmada gadoterik asit’in insan periferal lenfositlerinde in vitro genotoksik ve sitotoksik etkilerinin değerlendirilmesi amaçlandı. Çalışmada kardeş kromatid değişimi(KKD) yöntemi kullanıldı. KKD genotosisiteyi temsil eden, mitotik indeks (MI) sitotoksisiteyi temsileden parametreler olarak kullanıldı. Gadoteric acid’in 1, 2.5, 5, ve 25 mM konsantrasyonları ile 48 saatmuamele edilmiş insan periferal lenfositlerinde hem genotoksisite hem de sitototoksisite parametrelerideğerlendirildi. Gadoteric acid’in hiçbir konsantrasyonda MI değerleri üzerinde anlamlı bir değişikliğeneden olmadığı, KKD’ni ise sadece en yüksek konsantrasyonda (25 mM) anlamlı derecede arttığı tespitedildi (p

Evaluation of Gadoteric Acid's Effect on Sister Chromatid Exchange and Mitotic Index Under In Vitro Conditions

Gadoteric acid is extracellular gadolinium based contrast agent used for diagnosticpurposes in magnetic resonance (MR) imaging. In this study, it was aimed to evaluate the in vitro genotoxic and cytotoxic effects of gadoteric acid on human peripheral lymphocytes. Sister chromatidexchange (SCE) was used in the study. SCE and mitotic index (MI) were used as genotoxicity andcytotoxicity parameters respectively. Both genotoxicity and cytototoxicity parameters were evaluatedin human peripheral lymphocytes treated with 1, 2.5, 5, and 25 mM concentrations of gadoteric acid for48 hours. It was determined that gadoteric acid did not cause a significant change on MI values at anyconcentration and SCE values increased significantly only at the highest concentration (25 mM). Thesefindings indicate that gadoteric acid does not have a cytotoxic potential, but it may have a weakgenotoxic potential.

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