Sevim Feyza ERDOĞMUŞ, Şerife Nur DURMUŞ, Saniye ÖZDEMİR

Cistus creticus Ekstrelerinin Kontrollü Salımını Gerçekleştiren Katı Lipid Nanopartiküllerin (KLN) Geliştirilmesi ve Antimikrobiyal Ekinliklerinin Araştırılması

Bu çalışmanın amacı, tıbbi ve aromatik bitkiler arasında yer alan Cistus creticus ekstreleri yüklü kontrollü salım yapabilen katı lipid nanopartikül sistemleri geliştirmek ve bu sistemlerin patojen test mikroorganizmalara karşı antimikrobiyal etkinliğini araştırmaktır. Çalışma kapsamında C. creticus yapraklarından etanol ve su ekstresi ultrasonik yöntem kullanılarak elde edilmiştir. Bu ekstrelerinin yüklü olduğu katı lipid nanopartiküllerin karakterizasyonu SEM, DLS analizleri ve zeta potansiyelleri, in vitro salım profilleri ile değerlendirilmiştir. Etanol ve su ekstreleri yüklü katı lipid nanopartiküllerin boyutları sırasıyla 40-90 nm, zeta potansiyelleri –35.5 ve -30.7 mV ve PDI değerleri 0.455 ve 0.475 olarak saptanmıştır. Elde edilen katı lipid nanopartiküllerin patojen test mikroorganizmaları (Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRLB 4420. Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 51289, Escherichia coli ATCC 35218, Escherichia coli ATCC 25922) üzerindeki MİK değerleri 1.56-25 mg/mL aralığında değişiklik göstermektedir. Kuyu difüzyon testi sonuçlarına göre ekstre yüklü katı lipid nanopartiküllere karşı mikroorganizmalar, duyarlı, orta derecede duyarlı ve dirençli olarak belirlenmiştir. En yüksek antimikrobiyal etki etanol ekstresi yüklü nanopartiküller için zon çapı 19±0.75 olarak L. monocytogenes ATCC 19115’e karşı tespit edilmiştir.

Anahtar Kelimeler:

Antimikrobiyal aktivite, Cistus creticus, Katı lipid nanopartikül (KLN)

Development of Controlled Release of Cistus creticus Extracts Loaded Solid Lipid Nanoparticles (SLN) and Investigation of Their Antimicrobial Activities

This study aims to investigate the development extracts of Cistus creticus which is among medicinal and aromatic plants loaded with controlled delivery solid lipid nanoparticle systems and to investigate the antimicrobial activity of these systems against pathogen test microorganisms. Within the scope of this study, ethanol and water extracts from Cistus creticus leaves were obtained by using the ultrasonic method. The characterization of plant extracts loaded with solid lipid nanoparticles was evaluated by SEM, DLS analyses, and zeta potentials, in vitro release profiles. The sizes of ethanol and water plant extracts loaded solid lipid nanoparticles are respectively 40-90 nm, zeta potentials -35.5 and -30.7 mV and PDI values 0.455 and 0.475 were determined. The MIC values of the obtained solid lipid nanoparticles on pathogenic test microorganisms (Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRLB 4420. Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 51289, Escherichia coli ATCC 35218, Escherichia coli ATCC 25922) were determined as in the range of 56-25 mg/mL. According to the results of the good diffusion test, microorganisms were determined as susceptible, moderately sensitive, and resistant to the extract-loaded solid lipid nanoparticles. The highest antimicrobial effect was determined against L. monocytogenes ATCC 19115 with a zone diameter of 19±0.75 for ethanol extract-loaded nanoparticles.

Keywords:

Antimicrobial activity, Cistus creticus, Solid lipid nanoparticle (SLN),

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