Necdet AKAY, Tülay CANATAN, Onur YILMAZ, Nezih ATA, Orhan KARACA, İbrahim CEMAL

Yok Olma Tehlikesi Altındaki Güney Karaman Koyunlarda Genetik Çeşitlilik ve Genetik Darboğaz Analizleri

Bu çalışma, FAO tarafından önerilen onaltı mikrosatellit belirteç kullanılarak genetik kaynak olarak korunan Güney Karaman koyun popülasyonuna yönelik genetik çeşitlilik ve darboğaz analizlerinin gerçekleştirilmesi amacıyla gerçekleştirilmiştir. Çalışmanın hayvan materyalini Bahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü'nde yetiştirilen 119 Güney Karaman ırkı koyun oluşturmuştur. Çalışılan on altı mikrosatellit belirteçten toplam 277 allel tespit edilmiştir. Populasyon büyüklüğü çok sınırlı olmasına rağmen, ortalama alel sayısı (17.31), gözlemlenen heterozigotluk (0.81) ve polimorfik bilgi içeriği (0.82) bulguları, bu popülasyonda gözle görülür bir genetik çeşitlilik olduğuna işaret etmektedir. İncelenen on altı mikrosatellit belirteçin onunda pozitif FIS değeri elde edilmiştir. Ortalama FIS değeri 0.042 olmuştur. Genetik darboğazın tespiti için Bottleneck yazılımındaki sonsuz alel modeli (IAM), iki fazlı mutasyon modeli (TPM) ve aşamalı mutasyon modeli (SMM) gibi üç farklı model kullanılmıştır. Gerçekleştirilen darboğaz analizinden elde edilen L şeklindeki eğri, Güney Karaman koyun populasyonunda yakın zamanda herhangi bir genetik darboğaz olmadığını ortaya çıkarmıştır. Bu sonuçlar, Güney Karaman koyun populasyonu için koruma stratejilerinin geliştirilmesine yardımcı olacaktır.

Anahtar Kelimeler:

Genetik Çeşitlilik, Genetik Kaynak, Mikrosatellit, Populasyon yapısı

Genetic Diversity and Bottleneck Analysis of Endangered Güney Karaman Sheep

The present study was performed to reveal genetic variability of autochthonous Güney Karaman sheep breed, which is conserved as a genetic resource, using sixteen microsatellites marker recommended by FAO. Animal material for the study was consisted of 119 Güney Karaman sheep raised in Bahri Dagdas International Agricultural Research Institute. A total of 277 alleles were detected from sixteen markers studied. Although total population size is very limited, the mean number of alleles (17.31), observed heterozygosity (0.81) and polymorphic information content (0.82) findings indicated that noticeable genetic variability in this population. Ten out of the sixteen microsatellite markers studied had a positive FIS value. The mean value of FIS was 0.042. The infinite allele model (IAM), two-phase mutation model (TPM) and stepwise mutation model (SMM) in the Bottleneck software were used to check genetic bottleneck. The L-shaped curve obtained from analyze indicates absence of bottleneck in Güney Karaman sheep population. These results will help to develop conservation strategies for the Güney Karaman sheep population.

Keywords:

Genetic diversity, Genetic resource, Microsatellite, Population structure,

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