Yenidoğan Bir Buzağıda Zoonotik Campylobacter jejuni'nin İzolasyonu ve İdentifikasyonu

Campylobacteriozis tüm dünyada yaygın görülen zoonoz bir enfeksiyondur. Bu olguda; iştahsızlık ve ishal gözlenen, antibiyotik ve vitamin preperatları ile yapılan semptomatik tedaviye de cevap vermeyerek ölen yeni doğan (yedi günlük) buzağıda Campylobacter jejuni tanımlanmıştır. Otopsisinde akciğerlerde hiperemi, karaciğerde dejenerasyonlar, dalakta nekrotik odaklar, barsaklarda kataral ve hemorajik değişiklikler görülen buzağının dokularının histopatolojik incelemesinde, barsak epitellerinde yer yer dökülmeler görüldü .Akciğerlerinde bağdoku artışı, alveollerde dejenerasyon ve çevrelerinde lenfosit infiltrasyonu, bronşlarda yoğun bakteri kümeleri, damar endotellerinde dökülmeler görüldü. Karaciğerde portal boşluklarda lenfosit infiltrasyonu, damarlarda trombozlar ve çeperlerinde kalınlaşmalar, hepatositlerde erime nekrozu ve dejenerasyon, dalakta kas trabeküllerinde kalınlaşmalar, kalp kasında ödem gözlendi. Bakteriyolojik incelemelerde Campylobacter jejuni izole ve identifiye edildi.

The Isolation and Identification of Zoonotic Campylobacter jejuni from a Newborn Calf

Campylobacteriosis is a worldwide zoonotic infection. In this case report, Campylobacter jejuni was described in a newborn calf (seven days old) that had been suffered from diarrhea and loss of appetite and not responded to symptomatic antibiotic and vitamin treatments. In the necropsy, pulmonary hyperemia in the lungs, degenerations in the liver, focal necrosis in the spleen, cataral and necrotic alterations in the intestines were seen. In the histopathological examinations, desquamation in some areas of the intestinal epithelium of increases in the connective tissues of the lungs, degenerations in alveolus and lymphocyte infiltration around them, bacterial clumpsin bronches, disintegrations of endothelium of blood vessels were seen. Lymphocyte infiltrations in liver portal space, thrombosis in blood vessels and thickness in the blood vessel walls, degeneration in hepatocytes were seen. Thickness of spleen and muscle trabeculas, oedema in heart muscle were observed. In the bacteriological examinations, Campylobacter jejuni was isolated and identified.

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  • Açık MN, 2006: Sığır ve koyun orijinli Campylobacter jejuni ve Campylobacter coli'nin moleküler tiplendirilmesi. Doktora tezi, FÜ. Sağlık Bilimleri Enstitüsü, Elazığ.
  • Anonim, 1996: Bovine Genital Campylobacteriosis. Office International des Epizooties Manuel. 256-266.
  • Bancroft JD, Cook HC, 1984: Manual of Histological Techineques, New York, USA.
  • Broman T, Palmgren H, Bersröm S, Sellin M, Waldeström J, Danielsson-Tham ML & Olsen B, 2002: Campylobacter jejuni in black-headed gulls (Laurus ridibundus) : Prevalence, genotypes and inflüence on Campylobacter jejuni epidemiology. J. Clin Microbiol, 40, 4594-4602.
  • Diker KS, 1985: Koyun ve sığırlardan izole edilen Campylobacter türlerinin identifikasyonu üzerinde çalışmalar. Doğa Bilim Derg, 9(3): 232-240.
  • Diker KS, İstanbulluoğlu E, 1995: Sağlıklı ve sürgünlü hayvanlarda Campylobacter fetus subsp. jejuni izolasyonu üzerinde çalışmalar. A.Ü. Vet. Fak. Derg, 30, 28-32.
  • Fox JG, Post DE, Colin P, Ackerman JI, 1983: Campylobacter jejuni-assosiated diarrhea in dogs. J.Am. Vet. Med. Assoc, 183, 1430-1433.
  • Friedman CR, Neimann J, Wegener HC, Tauxe V, 2000 : Campylobacter, 2nd ed., ASM Press, Washington DC, USA, 121-138.
  • Harvey SM, 1980: Hippurat Hidrolysis by Campylobacter fetus. J.Cin Microbiol, 11: 435-437.
  • Kenar B, Erganiş O, 1994: Orta Karadeniz Bölgesi atık yapan koyunlarda Campylobacter türleri izole edilmesi ve antibiyotik duyarlığının tesbiti. Veterinarium, 5(1-2): 4-12.
  • Lastovica AJ, Skirrow MB, 2000 : Clinical significance of Campylobacter and related species other than Campylobacter. 2nd ed., ASM Press, Washington DC, USA.
  • Luna LG, 1968: Manual of Histologic Staining Methods of the Armed Forces İnstitue of Pathology .3rd ed., McGraw-Hill Book Company, New York, USA, 38- 220.
  • Mc Fadyean F, Stockman S, 1913: Report of the Departmental Committe appointed by the Board Agriculture and Fisheries to inguire into epizootic abortion. Ed; Rhoades HE, Appendix to Part 2: Abortion in Sheep. London, England.
  • Moore JE, Corcoran D, Dooley JS, Fanning S, Lucey B, Matsude M, McDowel DA, Megraud F, Millar BC, O'Mahony R, O'Riordan, O'Rourke M, Rao JR, Rooney PJ, Sails A, Whyte P, 2005: Campylobacter. Veterinary Research, 36,351-382.
  • Newel DG, Duim B, van Bergen MAP, Grogono-Thomas R, Wagenaar JA, 2000: Spesification, subspeciation and subtyping of Campylobacter fetus associated with infertility within the UK. Cattle Practice, 8, 421-425.
  • Patton CM, Nicholson MA, 1991: Evaluation of Disk Method for Hippurate Hydrolysisby Campylobacter species. J. Clin. Microbiol, 33(5), 1341-1343.
  • Skirrow MB, Benjamin J, 1980: "1001" Campylobacters : Culturel carecteristics of intestinal Campylobacter from men and animals. J. Hyg, 85:427-429.
  • Skirrow MB, Blaser MJ, 2000: Clinical aspects of Campylobacter infection. In: Campylobacter. 2nd ed., ASM Press, Washington DC, USA.
  • Tauxe RV, 1992: Epidemiology of Campylobacter jejuni infections in the United States and other industrialezed nations. In: Campylobacter jejuni: current state and future trends, Ed; Nachamkin I, Blaser MJ, Tompkins LS, ASM Press, Washington DC, USA.
  • Torre E, 1993: Factors infuencing shedding of Campylobacter jejuni in dogs without diarrhea. Am. J. Vet.Res, 54, 260-262.
  • Waldestrom J, Broman T, Carlsson I, Hasselquist D, Archterberg RP, Wagenaar JA, Olsen B, 2002: Prevalence Campylobacter jejuni, C. lari and C. coli in different ecological guilds and taxa of migrating birds. Appl. Environ. Microbiol, 68, 5911-5917.
Harran Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 2146-717X
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 2012
  • Yayıncı: HARRAN ÜNİVERSİTESİ VETERİNER FAKÜLTESİ