Mahmut SÖZMEN, Ebru Karadag SARI, İsmail Şah HAREM, Melek HAREM KOÇAK

Bıldırcın (Coturnix Coturnix Japonica) oviduktunda fibroblast büyüme

Bu çalışma bıldırcın oviduktunda VEGF ve FGF-2nin immunolokalizasyonunu belirlemek amacıyla yapıldı. İmmunohistokimyasal yöntem olarak avidin-biotin peroksidaz metodu uygulandı. VEGF ve FGF-2 immunboyanması infindibulumun kaudal bölümünün ve istmus bölümünün lumenini sınırlandıran epitelde oldukça yoğundu ve çoğunlukla apikal sitoplazmada lokalizeydi. İstmusu çevreleyen epitel hücrelerinde VEGF ve FGF-2 için diffuz sitoplazmik reaksiyon gözlendi. Ancak bu reaksiyon VEGF için çok yoğun, FGF-2 için ise lumen epitelinde çok zayıf, invagine olan epitel bölgelerinde ise orta yoğunluktaydı. Magnum bölümünde, lamina propriyada bulunan bezlerin daha aktif olduğu bölgelerdeki mukoza dürümlerini örten epitelde VEGF (+) hücrelere rastlandı. Uterus ve vagina bölümlerinde sadece muskularis katmanındaki düz kas dokusunda FGF-2 için orta ve zayıf derecede immunboyanma gözlendi. FGF-2 ve VEGFnin sağlıklı bıldırcın oviduktunda ekspresse edilmeleri, bu canlılarda üreme fizyolojisinin kontrolünde sadece hormonal mekanizmaların değil farklı diğer bazı moleküler mekanizmaların da bulunduğunu göstermektedir.

Expression of fibroblast growth factor-2 and vascular endothelial growth factor in the quail (Coturnix Coturnix Japonica) oviduct

This study was aimed at determining the immunolocalization of VEGF and FGF-2 in the quail oviduct. The avidinbiotin peroxidase method was applied as an immunohistochemical technique. Immunostaining for V EGF a nd F GF-2 was rather intense in the luminal epithelium of caudal region of the infundibulum and of the isthmus , and it was observed that immunostaining was localized mostly in the apical cytoplasm. The epithelial cells lining the isthmus displayed diffuse cytoplasmic reactions for VEGF and FGF-2. While the reaction for VEGF was very intense, the reaction for FGF-2 was very weak in the luminal epithelium and moderate in the invaginated epithelium. In the magnum, VEGF (+) cells were present in the epithelium lining the mucosal folds, which were situated in regions with higher activity of the proprial glands. In the uterus and vagina, moderate to weak immunostaining for FGF-2 was detected in only the smooth muscle of the muscularis layer. The expression of FGF-2 and VEGF in the oviduct of healthy quails demonstrates that the reproductive physiology of these animals is regulated not only by hormonal mechanisms, but also by other molecular mechanisms.

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