Sahabettin SELEK, Mehmet ASLAN, Yasar NAZLIGUL

Nonalkolik karaciğer yağlanması hastalığında oksidatif stres ve PON1 aktivitesi English

Amaç: Yapılan son çalışmalar erken atherosklerozun güvenilir bir belirtisi olan artmış karotis intima-media kalınlığı ile nonalkolik karaciğer yağlanması hastalığı arasında bir ilişki olduğunu ortaya koymuştur. Ancak, karotis arter aterosklerozu ile non-alkolik karaciğer yağlanması hastalığı arasında direk bir ilişki olup olmadığı bilinmemektedir. Bu nedenledir ki, bizler lipid hidroperoksit ile serum paraoksanaz ve arilesteraz aktivitelerini PON1 araştırmayı amaç edindik.Materyal ve metod: Çalışmamıza karaciğer biopsileri alınmış 32 hasta ve 28 sağlıklı ve gönüllü kontrol grubu dahil edildi. Serum bazal ve tuz ile uyarılmış paraoksanaz ve arilesteraz aktiviteleri spektrofotometrik olarak ölçülürken, lipid hidroperoksit seviyeleri ise FOX-2 metodu ile çalışıldı.Bulgular: Lipid hidroperoksit seviyeleri control grubu ile karşılaştırıldığında hasta grubunda daha yüksek iken p

Anahtar Kelimeler:

PON1 aktivitesi, lipid hidroperoksit, ateroskleroz, nonalkolik karaciğer yağlanması

Serum PON1 activity and oxidative stress in non-alcoholic fatty liver disease

Background: Recent studies have shown that non-alcoholic fatty liver disease NAFLD is associated with increased carotid intima-media thickness as a reliable marker of early atherosclerosis, but possible direct relationships between NAFLD and carotid artery atherosclerosis is unknown. Therefore, the aim of this study was to investigate serum paraoxonase and arylesterase activities PON1 along with lipid hydroperoxide LOOH levels.Methods: We studied 32 consecutive patients with biopsy proven NAFLD and 28 healthy controls. Serum basal/saltstimulated paraoxonase and arylesterase activities were detected spectrophotometrically. LOOH levels were measured by the FOX–2 assay. Results: Serum basal/salt-stimulated paraoxonase and arylesterase activities were significantly lower in NAFLD than controls p

Keywords:

PON1 activity, lipid hydroperoxide, atherosclerosis, non-alcoholic fatty liver disease,

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